The molecular mechanism of light-induced germination in resting spores of a coastal diatom

• Project/Area Number: 26450253
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Aquatic bioproduction science
• Research Institution: Fisheries Research and Education Agency
• Principal Investigator: Shikata Tomoyuki 国立研究開発法人水産研究・教育機構, 瀬戸内海区水産研究所, 研究員 (40603048)
• Co-Investigator(Kenkyū-buntansha): 得津 隆太郎 基礎生物学研究所, 環境光生物学研究部門, 助教 (60613940)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 赤潮 / 光 / 光合成 / プロテオーム解析 / 発芽 / 休眠胞子 / 珪藻 / 休眠期細胞 / 光発芽 / 全mRNA-seq / 生活環

Outline of Final Research Achievements

We examined the molecular mechanism of light-induced germination in resting spore of a coastal diatom. Photosynthetic activity of resting spore increased just after light-on. Quantity of photosynthetic proteins scarcely changed after light-on, but ATPase increased. Furthermore, proteome analysis indicated that proteins involved in citric cycle, glycometabolism, singling and nutritional metabolism are newly expressed after light-on. These results suggest a scenario that the resting spore used energy to activate nutritional metabolism and so on after light-on, and then photosynthesize to elongate and germinate.

Research Products

• [Journal Article] Relationships between light environment and subsurface accumulation during the daytime in the red-tide dinoflagellate Karenia mikimotoi (2017)
  • Author(s): Shikata T et al.
  • Journal Title: Marine Biology Volume: 164 Issue: 1 Pages: 18-18
  • DOI: 10.1007/s00227-016-3042-4
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Light spectrum regulates cell accumulation during daytime in the raphidophyte Chattonella antiqua causing noxious red tides (2016)
  • Author(s): Shikata, T., Matsunaga, S., Kuwahara, Y., Iwahori, S. and Nishiyama, Y.
  • Journal Title: J. Photochem. Photobiol. B Biol. Volume: 160 Pages: 128-133
  • DOI: 10.1016/j.jphotobiol.2016.03.046
  • Peer Reviewed
• [Journal Article] Factors influencing maintenance and decline of a diatom bloom in the Yatsushiro Sea, Japan. (2016)
  • Author(s): Goh Onitsuka, Tomoyuki Shikata, Saho Kitatsuji, Kazuo Abe, Takeshi Yamamoto, Hiroki Ochiai, Hitoshi Matsuo
  • Journal Title: Journal of Oceanography Volume: 72 Issue: 4 Pages: 1-11
  • DOI: 10.1007/s10872-016-0358-0
  • NAID: 40020893178
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Effects of temperature, salinity, and photosynthetic photon flux density on the growth of the harmful diatom Asteroplanus karianus in the Ariake Sea, Japan. (2015)
  • Author(s): Shikata, T., Matsubara, T., Yoshida, M., Sakamoto, S., & Yamaguchi, M.
  • Journal Title: Fisheries Science Volume: 81 Issue: 6 Pages: 1063-1069
  • DOI: 10.1007/s12562-015-0930-3
  • NAID: 40020653553
  • Peer Reviewed / Acknowledgement Compliant
• [Book] 有害有毒プランクトンの科学 (2016)
  • Author(s): 紫加田 知幸
  • Total Pages: 341
  • Publisher: 恒星社厚生閣