| Project/Area Number |
26450253
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Aquatic bioproduction science
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| Research Institution | Fisheries Research and Education Agency |
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Principal Investigator |
Shikata Tomoyuki 国立研究開発法人水産研究・教育機構, 瀬戸内海区水産研究所, 研究員 (40603048)
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| Co-Investigator(Kenkyū-buntansha) |
得津 隆太郎 基礎生物学研究所, 環境光生物学研究部門, 助教 (60613940)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 赤潮 / 光 / 光合成 / プロテオーム解析 / 発芽 / 休眠胞子 / 珪藻 / 休眠期細胞 / 光発芽 / 全mRNA-seq / 生活環 |
|---|
| Outline of Final Research Achievements |
We examined the molecular mechanism of light-induced germination in resting spore of a coastal diatom. Photosynthetic activity of resting spore increased just after light-on. Quantity of photosynthetic proteins scarcely changed after light-on, but ATPase increased. Furthermore, proteome analysis indicated that proteins involved in citric cycle, glycometabolism, singling and nutritional metabolism are newly expressed after light-on. These results suggest a scenario that the resting spore used energy to activate nutritional metabolism and so on after light-on, and then photosynthesize to elongate and germinate.
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