| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
Fugu helper T (Th) cells expressed cytokines such as interleukin (IL)-4/13 and interferon (IFN)-gamma related to Th cell differentiation by the stimulation of recombinant Fugu IL-4/13 and IFN-gamma. And there was a difference in the induced expression patterns of cytokines between recombinant IL-4/13 and IFN-gamma stimulated cells. Analysis of cytokine production by the stimulated cells using intracellular cytokine staining assay suggested that Fugu has similar effecter Th cells with that of mammalian species. In addition, IL-4/13 and IFN-gamma were produced by the Th cells activated monocytes. Moreover, Th cells stimulated by LPS or chitosan differentiated into each different effecter Th cells. From these results, it was suggested that Th cells in fish can be used as markers of fish disease diagnosis.
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