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Development of basic technology for production of tiger pufferfish with low teeth formation to prevent mutual bite.

Research Project

Project/Area Number 26450273
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Aquatic bioproduction science
Research InstitutionFisheries Research and Education Agency

Principal Investigator

OKAMOTO Hiroyuki  国立研究開発法人水産研究・教育機構, 増養殖研究所, グループ長 (50372040)

Co-Investigator(Kenkyū-buntansha) 藤原 篤志  国立研究開発法人水産研究・教育機構, 中央水産研究所, 主幹研究員 (30443352)
木下 政人  京都大学, 農学研究科, 助教 (60263125)
Research Collaborator YAMAMOTO Takashi  広島大学, 大学院理学研究科, 教授
SAKUMA Tetsushi  広島大学, 大学院理学研究科, 特任講師
ISHIKAWA Takashi  国立研究開発法人水産研究・教育機構, 増養殖研究所, 研究支援職員
KAI Wataru  元国立研究開発法人水産研究・教育機構, 中央水産研究所, 研究支援職員
NISHIKI Issei  国立研究開発法人水産研究・教育機構, 中央水産研究所, 研究支援職員
IWASAKI Yuki  元国立研究開発法人水産研究・教育機構, 中央水産研究所, 研究支援職員
KISHIMOTO Kenta  京都大学, 大学院農学研究科
Murakami Yu  京都大学, 大学院農学研究科
OKADA Kazuhiro  三重県尾鷲栽培漁業センター, 所長
KASUYA Tohru  三重県尾鷲栽培漁業センター, 技師
SUGIYAMA Syouhei  三重県尾鷲栽培漁業センター, 技師
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywordsトラフグ / 切歯形成 / 突然変異 / ゲノム編集 / 切歯形成遺伝子 / 遺伝子編集 / CRISPR/Cas / 顕微注入 / 噛み合い
Outline of Final Research Achievements

In order to reduce tooth cutting procedure to prevent mutual bite in tiger pufferfish farming, we developed the basic technology for creating individuals whose function of nine SCPP genes involved in tooth formation was suppressed. The mutants in the genes were comprehensively searched from about 850 individual which were raised from the mutagenesis treatment male, but mutants with the suppressed function were not obtained. On the other hand, by genomic editing technology we succeeded in producing pufferfish with mutations in one of SCPP genes, and the analysis using variant homozygote individuals is expected in the future.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (4 results)

All 2016 2015 2014

All Presentation (4 results) (of which Int'l Joint Research: 2 results,  Invited: 1 results)

  • [Presentation] Mutagenesis and genome editing for aquaculture fish species2016

    • Author(s)
      Hiroyuki Okamoto
    • Organizer
      44th Scientific Symposium of the UJNR Aquaculture Panel Genetics and Breeding in Aquaculture Symposium
    • Place of Presentation
      アメリカ、シアトル NOAA Northwest Fisheries Science Center
    • Year and Date
      2016-11-01
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Application of mutant screening for multi-genes using next generation sequencing technology from mutagenized populations2015

    • Author(s)
      Okamoto H., Ishikawa T., Nishiki I., Iwasaki Y., Kai W. and Fujiwara A.
    • Organizer
      International Symposium Genetics In Aquaculture
    • Place of Presentation
      スペイン、サンチャゴ・デ・コンポステラ大学
    • Year and Date
      2015-06-21 – 2015-06-27
    • Related Report
      2014 Research-status Report
  • [Presentation] Application of mutant screening for multi-genes using next generation sequencing technology from mutagenized populations2015

    • Author(s)
      Okamoto H., Ishikawa T., Nishiki I., Iwasaki Y., Kai W. and Fujiwara A.
    • Organizer
      International Symposium Genetics In Aquaculture
    • Place of Presentation
      スペイン、サンチャゴ・デ・コンポステラ大学
    • Year and Date
      2015-06-21
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] ゲノム編集によるトラフグの育種戦略2014

    • Author(s)
      岡本裕之
    • Organizer
      日本水産学会
    • Place of Presentation
      九州大学
    • Year and Date
      2014-09-19
    • Related Report
      2014 Research-status Report
    • Invited

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Published: 2014-04-04   Modified: 2018-03-22  

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