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Control of histamine-producing bacteria using bacteriophages

Research Project

Project/Area Number 26450278
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Aquatic life science
Research InstitutionHokkaido University

Principal Investigator

YAMAZAKI Koji  北海道大学, 水産科学研究院, 准教授 (40250500)

Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Keywordsヒスタミン / ヒスタミン生成菌 / バクテリオファージ / 微生物制御 / ヒスタミン食中毒 / ファージセラピー
Outline of Final Research Achievements

This study was investigated the characterization of newly isolated bacteriophages infected to histamaine-producing Morganella morganii and Photobacterium damselae, and utilization of bacteriophages for control of the pathogens and histamine accumulation. Three strains, FSP1, ΦMV-1 and ΦMV-4, for M.morganii and a strain, Phda1, for P.damselae were successfully isolated from river waters in this study. The viable count of M. morganii and histamine accumulation inhibited on tuna meat by the FSP1 treatment. This result suggests that bacteriophages such as FSP1 and Phda1 could be used as a biocontrol agent against M. morganii and P.damselae for treatment of infectious disease treatment or food decontamination.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (13 results)

All 2017 2016 2015 2014

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (12 results) (of which Int'l Joint Research: 3 results,  Invited: 1 results)

  • [Journal Article] Characterization of a novel bacteriophage Phda1 active against histamine-producing Photobacterium damselae subsp. damselae2015

    • Author(s)
      Shogo Yamaki, Yuji Kawai, Koji Yamazaki
    • Journal Title

      Journal of Applied Microbiology

      Volume: 118 Issue: 6 Pages: 1541-1550

    • DOI

      10.1111/jam.12809

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] Morganella morganii 溶菌バクテリオファージ FSP1を利用したマグロ魚肉によるヒスタミン食中毒のリスク低減2017

    • Author(s)
      山木将悟,川合祐史,山崎浩司
    • Organizer
      日本食品科学工学会2017年北海道支部大会
    • Place of Presentation
      北海道大学国際交流会館,北海道札幌市
    • Related Report
      2016 Annual Research Report
  • [Presentation] Utiization of bacteriophages for the inhibition of histamine accumulation by histamine-producing bacteria on fishery products2016

    • Author(s)
      Shogo Yamaki, Koji Yamazaki, Yuji Kawai
    • Organizer
      9th Joint Symposium on Food Scienece and Technology among NUS, TUMSAT and HU
    • Place of Presentation
      北海道大学水産学部,北海道函館市
    • Year and Date
      2016-12-01
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Photobacterium damselae subsp. damselae 溶菌ファージPhda10株の感染特性を利用したヒスタミン生成抑制2016

    • Author(s)
      山木将悟,川合祐史,山崎浩司
    • Organizer
      日本食品科学工学会第63回大会
    • Place of Presentation
      名城大学,愛知県名古屋市
    • Year and Date
      2016-08-25
    • Related Report
      2016 Annual Research Report
  • [Presentation] Isolation and partial characterization of two bacteriophages ΦMV-1 and ΦMV-4 of histamine-producing Morganella morganii2016

    • Author(s)
      Koji Yamazaki, Soya Kuronuma, Shogo Yamaki, Yuji Kawai
    • Organizer
      The IFT meeting
    • Place of Presentation
      McCormic Place South, Chicago, USA
    • Year and Date
      2016-07-16
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Isolation of bacteriophage Phda10 and combined effect of Phda10 and calcium acetate for growth inhibition of histamine-producing Photobacterium damselae subsp. damselae2016

    • Author(s)
      Shogo Yamaki, Yuji Kawai, Koji Yamazaki
    • Organizer
      The IFT meeting
    • Place of Presentation
      McCormic Place South, Chicago, USA
    • Year and Date
      2016-07-16
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 広宿主域を示すPhotobacterium damselae subsp damselae溶菌バクテリオファージの探索とその性状2015

    • Author(s)
      山木将悟,黒沼宗弥,川合祐史,山崎浩司
    • Organizer
      日本食品科学工学会第62回大会
    • Place of Presentation
      京都大学,京都府京都市
    • Year and Date
      2015-08-29
    • Related Report
      2015 Research-status Report
  • [Presentation] ファージカクテルによるヒスタミン産生Morganella morganiiの発育抑制2015

    • Author(s)
      黒沼宗弥,山木将悟,久保田光也,川合祐史,山崎浩司
    • Organizer
      日本食品科学工学会第62回大会
    • Place of Presentation
      京都大学,京都府京都市
    • Year and Date
      2015-08-29
    • Related Report
      2015 Research-status Report
  • [Presentation] 微生物由来の抗菌物質を利用した食品微生物制御2015

    • Author(s)
      山崎浩司
    • Organizer
      日本食品科学工学会北海道支部大会シンポジウム
    • Place of Presentation
      ロワジールホテル函館,北海道函館市
    • Year and Date
      2015-02-28
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] Growth inhibition of food-borne pathogens using biopreservatives of microbial origin in food2014

    • Author(s)
      Koji Yamazaki
    • Organizer
      The First Myanmar-Japan Symposium
    • Place of Presentation
      Pathein University, Pathein, Myanmar
    • Year and Date
      2014-11-30
    • Related Report
      2014 Research-status Report
  • [Presentation] Morganella morganii溶菌性バクテリオファージFSP1 による食品中におけるヒスタミン蓄積抑制2014

    • Author(s)
      大町拓生,山木将悟,黒沼宗弥,山崎浩司,川合祐史
    • Organizer
      日本食品科学工学会第61回大会
    • Place of Presentation
      中村学園大学,福岡県福岡市
    • Year and Date
      2014-08-30
    • Related Report
      2014 Research-status Report
  • [Presentation] Morganella morganii 溶菌性 Siphoviridae科バクテリオファージΦMV-1 の性状2014

    • Author(s)
      黒沼宗弥,山木将悟,大町拓生,山崎浩司,川合祐史
    • Organizer
      日本食品科学工学会第61回大会
    • Place of Presentation
      中村学園大学,福岡県福岡市
    • Year and Date
      2014-08-30
    • Related Report
      2014 Research-status Report
  • [Presentation] Isolation and characterization of a bacteriophage Phda1 active against histamine-producing Photobacterium damselae subsp. damselae2014

    • Author(s)
      Shogo Yamaki, Takuo Ohmachi, Souya Kuronuma, Yuji Kawai, Koji Yamazaki
    • Organizer
      The IFT Annual Meeting
    • Place of Presentation
      Morial Convention Center, New Orleans, USA
    • Year and Date
      2014-06-23
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2018-03-22  

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