Transcriptional profiling of olfactory inprinting-related molecules in chum salmon brain using next-generation sequencer

• Project/Area Number: 26450279
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Aquatic life science
• Research Institution: Hokkaido University
• Principal Investigator: Kudo Hideaki 北海道大学, 水産科学研究院, 准教授 (40289575)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: サケ / 母川回帰 / 嗅覚 / 刷込 / 脳 / 嗅球 / 終脳 / 次世代シーケンサー / 母川刷込 / トランスクリプトーム / 記憶

Outline of Final Research Achievements

In this study, genes involved in olfactory imprinting (OI) and memory retrieval (OMR) were searched in the chum salmon olfactory center. Transcriptome analyses using next-generation sequencer were carried out on olfactory center of the seaward migration (OI period) juveniles and the homeward migration (OMR period) adult salmon. In each period of seaward and homeward migration, these genes indicates the minimum expression at the silent stage, and indicated expression higher than 10 fold from silent stage at active stage. In this selective condition, the same genes were not contained in both selected transcripts. The molecules of pre-synaptic vesicle exocytosis, cell-growth/differentiation, etc. were included in the OI-related candidate genes. On the other hand, some neurotransmitter receptors were included in the OMR-related candidate genes. Our results suggest that different gene groups are mainly functioning to OI and OMR in Pacific salmon.

Research Products

• [Journal Article] 北海道の自然産卵サケ類における耳石微量元素分析による回遊履歴情報の資源管理への応用 ～自然産卵後斃死サクラマス耳石におけるストロンチウム：カルシウム比動態の河川間比較～. (2016)
  • Author(s): 工藤秀明, 越野陽介, 藤原 真, 青山智哉, 伊藤洋満
  • Journal Title: 公益財団法人 北水協会 水産研究助成事業報告 Volume: 平成27年度 Pages: 1-6
• [Journal Article] Histological characteristics of the oocyte chorion in wild post-spawning and artificially matured Japanese eels Anguilla japonica (2015)
  • Author(s): Izumi H., Hagihara Seishi, Kurogi H., Chow S., Tsukamoto K., Kagawa H., Kudo H., Ijiri S., Adachi S.
  • Journal Title: Fisheries Science Volume: 81 Issue: 2 Pages: 321-329
  • DOI: 10.1007/s12562-015-0851-1
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] 嗅粘膜における薬物代謝関連分子の発現とその意義 ―ニオイ受容器で異物を処理する細胞たち－. (2015)
  • Author(s): 工藤秀明
  • Journal Title: Aroma Research Volume: 16 Pages: 214-218
• [Journal Article] Dorsal hump morphology in pink salmon (Oncorhynchus gorbuscha) (2014)
  • Author(s): Kanta susuki, Masaki Ichimura, Yosuke Koshino, Masehide Kaeriyama, Yasuyuki Takagi, Shinji Adechi, Kudo Hideaki
  • Journal Title: Journal of Morphology Volume: 275 Issue: 5 Pages: 514-527
  • DOI: 10.1002/jmor.20234
  • Peer Reviewed
• [Presentation] サケ母川刷込関連時期の嗅覚中枢におけるシナプトソーム関連タンパク25 (SNAP25) の遺伝子発現 (2017)
  • Author(s): 阿部嵩志・小林正起・瀬部光太郎・工藤秀明
  • Organizer: 平成29年度日本水産学会春季大会
  • Place of Presentation: 東京海洋大学（東京都港区）
  • Year and Date: 2017-03-27
• [Presentation] 北海道太平洋側で繁殖するウトウによるサケ幼稚魚捕食量の推定 (2017)
  • Author(s): 大門純平・綿貫豊・工藤秀明・越野陽介
  • Organizer: 平成29年度日本水産学会春季大会
  • Place of Presentation: 東京海洋大学（東京都港区）
  • Year and Date: 2017-03-27
• [Presentation] Transcriptional profiling of olfactory imprinting in the olfactory center of Pacific salmon (2016)
  • Author(s): Yui Minowa, Seishi Hagihara, Takashi Abe,Shigeho Ijiri, Shinji Adachi and Hideaki Kudo
  • Organizer: 17th International Symposium on Olfaction and Taste (ISOT2016)
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2016-06-05
  • Int'l Joint Research
• [Presentation] Expression of synaptosome-associated protein 25 kDa (SNAP25) in the salmon brain (2016)
  • Author(s): Takashi Abe, Yui Minowa, Masaki Kobayashi, and Hideaki Kudo
  • Organizer: 17th International Symposium on Olfaction and Taste (ISOT2016)
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2016-06-05
  • Int'l Joint Research
• [Presentation] カラフトマスの鼻曲がり形成への雄性ホルモンの関与 (2016)
  • Author(s): 木村知彰・瀬部光太郎・市村政樹 ・井尻成保・工藤秀明・桜井泰憲
  • Organizer: 平成28年度日本水産学会春季大会
  • Place of Presentation: 東京海洋大学、東京都、港区
  • Year and Date: 2016-03-27
• [Presentation] サケの嗅覚機構 (2015)
  • Author(s): 工藤秀明
  • Organizer: 第9回サケ学研究会
  • Place of Presentation: 北海道大学、北海道、札幌市
  • Year and Date: 2015-12-20
• [Presentation] サケ前脳における母川想起関連分子の探索 (2015)
  • Author(s): 箕輪ゆい・萩原聖士・井尻成保・工藤秀明・足立伸次
  • Organizer: 第9回サケ学研究会
  • Place of Presentation: 北海道大学、北海道、札幌市
  • Year and Date: 2015-12-20
• [Presentation] カラフトマス鼻曲がり形成と雄性ホルモン (2015)
  • Author(s): 木村知彰・瀬部光太郎・市村政樹 ・井尻成保・工藤秀明・桜井泰憲
  • Organizer: 第9回サケ学研究会
  • Place of Presentation: 北海道大学、北海道、札幌市
  • Year and Date: 2015-12-20
• [Presentation] Expression of Golf α subunit in the olfactory organ of chum salmon（Oncorhynchus keta） (2014)
  • Author(s): 箕輪ゆい・福間 健・亀田圭介・國重賢二・工藤秀明
  • Organizer: 日本味と匂学会第48回大会
  • Place of Presentation: 清水文化会館マリナート、静岡市、静岡県
  • Year and Date: 2014-10-02 – 2014-10-04