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Transcriptional profiling of olfactory inprinting-related molecules in chum salmon brain using next-generation sequencer

Research Project

Project/Area Number 26450279
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Aquatic life science
Research InstitutionHokkaido University

Principal Investigator

Kudo Hideaki  北海道大学, 水産科学研究院, 准教授 (40289575)

Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywordsサケ / 母川回帰 / 嗅覚 / 刷込 / 脳 / 嗅球 / 終脳 / 次世代シーケンサー / 母川刷込 / トランスクリプトーム / 記憶
Outline of Final Research Achievements

In this study, genes involved in olfactory imprinting (OI) and memory retrieval (OMR) were searched in the chum salmon olfactory center. Transcriptome analyses using next-generation sequencer were carried out on olfactory center of the seaward migration (OI period) juveniles and the homeward migration (OMR period) adult salmon. In each period of seaward and homeward migration, these genes indicates the minimum expression at the silent stage, and indicated expression higher than 10 fold from silent stage at active stage. In this selective condition, the same genes were not contained in both selected transcripts. The molecules of pre-synaptic vesicle exocytosis, cell-growth/differentiation, etc. were included in the OI-related candidate genes. On the other hand, some neurotransmitter receptors were included in the OMR-related candidate genes. Our results suggest that different gene groups are mainly functioning to OI and OMR in Pacific salmon.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (13 results)

All 2017 2016 2015 2014

All Journal Article (4 results) (of which Peer Reviewed: 2 results,  Acknowledgement Compliant: 1 results) Presentation (9 results) (of which Int'l Joint Research: 2 results)

  • [Journal Article] 北海道の自然産卵サケ類における耳石微量元素分析による回遊履歴情報の資源管理への応用 ~自然産卵後斃死サクラマス耳石におけるストロンチウム:カルシウム比動態の河川間比較~.2016

    • Author(s)
      工藤秀明, 越野陽介, 藤原 真, 青山智哉, 伊藤洋満
    • Journal Title

      公益財団法人 北水協会 水産研究助成事業報告

      Volume: 平成27年度 Pages: 1-6

    • Related Report
      2016 Annual Research Report
  • [Journal Article] Histological characteristics of the oocyte chorion in wild post-spawning and artificially matured Japanese eels Anguilla japonica2015

    • Author(s)
      Izumi H., Hagihara Seishi, Kurogi H., Chow S., Tsukamoto K., Kagawa H., Kudo H., Ijiri S., Adachi S.
    • Journal Title

      Fisheries Science

      Volume: 81 Issue: 2 Pages: 321-329

    • DOI

      10.1007/s12562-015-0851-1

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] 嗅粘膜における薬物代謝関連分子の発現とその意義 ―ニオイ受容器で異物を処理する細胞たち-.2015

    • Author(s)
      工藤秀明
    • Journal Title

      Aroma Research

      Volume: 16 Pages: 214-218

    • Related Report
      2015 Research-status Report
  • [Journal Article] Dorsal hump morphology in pink salmon (Oncorhynchus gorbuscha)2014

    • Author(s)
      Kanta susuki, Masaki Ichimura, Yosuke Koshino, Masehide Kaeriyama, Yasuyuki Takagi, Shinji Adechi, Kudo Hideaki
    • Journal Title

      Journal of Morphology

      Volume: 275 Issue: 5 Pages: 514-527

    • DOI

      10.1002/jmor.20234

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Presentation] サケ母川刷込関連時期の嗅覚中枢におけるシナプトソーム関連タンパク25 (SNAP25) の遺伝子発現2017

    • Author(s)
      阿部嵩志・小林正起・瀬部光太郎・工藤秀明
    • Organizer
      平成29年度日本水産学会春季大会
    • Place of Presentation
      東京海洋大学(東京都港区)
    • Year and Date
      2017-03-27
    • Related Report
      2016 Annual Research Report
  • [Presentation] 北海道太平洋側で繁殖するウトウによるサケ幼稚魚捕食量の推定2017

    • Author(s)
      大門純平・綿貫豊・工藤秀明・越野陽介
    • Organizer
      平成29年度日本水産学会春季大会
    • Place of Presentation
      東京海洋大学(東京都港区)
    • Year and Date
      2017-03-27
    • Related Report
      2016 Annual Research Report
  • [Presentation] Transcriptional profiling of olfactory imprinting in the olfactory center of Pacific salmon2016

    • Author(s)
      Yui Minowa, Seishi Hagihara, Takashi Abe,Shigeho Ijiri, Shinji Adachi and Hideaki Kudo
    • Organizer
      17th International Symposium on Olfaction and Taste (ISOT2016)
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Year and Date
      2016-06-05
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Expression of synaptosome-associated protein 25 kDa (SNAP25) in the salmon brain2016

    • Author(s)
      Takashi Abe, Yui Minowa, Masaki Kobayashi, and Hideaki Kudo
    • Organizer
      17th International Symposium on Olfaction and Taste (ISOT2016)
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Year and Date
      2016-06-05
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] カラフトマスの鼻曲がり形成への雄性ホルモンの関与2016

    • Author(s)
      木村知彰・瀬部光太郎・市村政樹 ・井尻成保・工藤秀明・桜井泰憲
    • Organizer
      平成28年度日本水産学会春季大会
    • Place of Presentation
      東京海洋大学、東京都、港区
    • Year and Date
      2016-03-27
    • Related Report
      2015 Research-status Report
  • [Presentation] サケの嗅覚機構2015

    • Author(s)
      工藤秀明
    • Organizer
      第9回サケ学研究会
    • Place of Presentation
      北海道大学、北海道、札幌市
    • Year and Date
      2015-12-20
    • Related Report
      2015 Research-status Report
  • [Presentation] サケ前脳における母川想起関連分子の探索2015

    • Author(s)
      箕輪ゆい・萩原聖士・井尻成保・工藤秀明・足立伸次
    • Organizer
      第9回サケ学研究会
    • Place of Presentation
      北海道大学、北海道、札幌市
    • Year and Date
      2015-12-20
    • Related Report
      2015 Research-status Report
  • [Presentation] カラフトマス鼻曲がり形成と雄性ホルモン2015

    • Author(s)
      木村知彰・瀬部光太郎・市村政樹 ・井尻成保・工藤秀明・桜井泰憲
    • Organizer
      第9回サケ学研究会
    • Place of Presentation
      北海道大学、北海道、札幌市
    • Year and Date
      2015-12-20
    • Related Report
      2015 Research-status Report
  • [Presentation] Expression of Golf α subunit in the olfactory organ of chum salmon(Oncorhynchus keta)2014

    • Author(s)
      箕輪ゆい・福間 健・亀田圭介・國重賢二・工藤秀明
    • Organizer
      日本味と匂学会第48回大会
    • Place of Presentation
      清水文化会館マリナート、静岡市、静岡県
    • Year and Date
      2014-10-02 – 2014-10-04
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2018-03-22  

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