Studies on mechanisms underlying formation of lipid droplets in oocytes of teleost fishes

• Project/Area Number: 26450280
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Aquatic life science
• Research Institution: Hokkaido University
• Principal Investigator: Todo Takashi 北海道大学, 水産科学研究院, 准教授 (60303111)
• Co-Investigator(Renkei-kenkyūsha): HARA Akihiko 北海道大学, 大学院水産科学研究院, 名誉教授 (40091483) ; HIRAMATSU Naoshi 北海道大学, 大学院水産科学研究院, 准教授 (10443920)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 卵形成 / 卵母細胞 / 卵成長 / 中性脂質 / 油球 / 卵濾胞 / リポタンパク / リポタンパク質 / 生理 / 魚類 / 卵黄

Outline of Final Research Achievements

We have previously shown about the mechanisms underlying formation of lipid droplets in teleost oocytes as follows; plasma very low density lipoprotein is the major carrier of neutral lipids to oocyte lipid droplets, and Vldl is metabolized at the outside of oocyte, then free fatty acids derived from the Vldl metabolism are incorporated into oocyte. In this study, we focused on the role of lipoprotein lipase (Lpl), a key enzyme of Vldl metabolism, and analyzed its expression pattern in ovarian follicle using salmonids, medaka, pipefish and zebrafish. As the results, it was suggested that the presence of Lpl expressed in ovarian follicle cells is indispensable for the formation of lipid droplets in oocytes.

Research Products

• [Journal Article] Molecular cloning and characterization of hagfish estrogen receptors (2017)
  • Author(s): Nishimiya, T., Katsu, Y., Inagawa, H., Hiramatsu, N., Todo, T., Hara, A.
  • Journal Title: J. Steroid Biochem. Mol. Biol. Volume: 165 Pages: 190-201
  • DOI: 10.1016/j.jsbmb.2016.06.004
  • Peer Reviewed
• [Journal Article] アムールチョウザメAcipenser schrenckiiの多型ビテロジェニン遺伝子のcDNAクローニング (2016)
  • Author(s): 前林衛・稲岡雄平・吉田達哉・萩原聖士・西宮攻・莚平裕次・原彰彦・東藤孝・平松尚志
  • Journal Title: 水産増殖 Volume: 64 Pages: 63-76
  • NAID: 130005464546
  • Peer Reviewed
• [Journal Article] Ovarian yolk formation in fishes: Molecular mechanisms underlying formation of lipid droplets and vitellogenin-derived yolk proteins (2015)
  • Author(s): Hiramatsu N, Todo T, Sullivan CV, Schilling J, Reading BJ, Matsubara T, Ryu YW, Mizuta H, Luo W, Nishimiya O, Wu M, Mushirobira Y, Yilmaz O, Hara A
  • Journal Title: General and Comparative Endocrinology Volume: ７ Pages: 9-15
  • DOI: 10.1016/j.ygcen.2015.01.025
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Molecular cloning and partial characterization of a low-density lipoprotein receptor-related protein 13 (LRP13) involved in vitellogenin uptake in the cutthroat trout (Oncorhynchus clarki). (2015)
  • Author(s): Mushirobira, Y., Mizuta, H., Luo, W., Todo, T., Hara, A., Reading, B.J., Sullivan, C.V., Hiramatsu, N.
  • Journal Title: Mol. Reprod. Dev. Volume: 82 Issue: 12 Pages: 986-1000
  • DOI: 10.1002/mrd.22579
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Ovarian yolk formation in fishes: Molecular mechanisms underlying formation of lipid droplets and vitellogenin-derived yolk proteins. (2015)
  • Author(s): Hiramatsu, N., Todo, T., Sullivan, C., Svilling, J., Reading, B.J., Matsubara, T., Ryu, Y.-W., Mizuta, H., Luo, W., Nishimiya, O., Wu, M., Mushirobira, Y., Yilmaz, O., Hara, A.
  • Journal Title: Gen. Comp. Endocrinol Volume: 印刷中
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Lrp13 is a novel vertebrate lipoprotein receptor that binds vitellogenin in teleost fishes. (2014)
  • Author(s): Reading, B.J., Hiramatsu, N., Schilling, J., Molloy, K., Glassbrook, N., Mizuta, H., Luo, W., Baltzegar, D.A., Williams, V.N., Todo, T., Hara, A., Sullivan, C.V.
  • Journal Title: J. Lipid Res. Volume: 55 Issue: 11 Pages: 2287-2295
  • DOI: 10.1194/jlr.m050286
  • Peer Reviewed
• [Journal Article] Molecular cloning and characterization of the expression profiles of vitellogenin transcripts in the dojo loach (Misgurnus anguillicaudatus) in response to 17α-ethinylestradiol and 17β-estradiol administration (2014)
  • Author(s): Wu M, Nishimiya O, Nakamori M, Soyano K, Todo T, Hara A, Hiramatsu N
  • Journal Title: Zoological Science Volume: 31(4) Issue: 4 Pages: 202-212
  • DOI: 10.2108/zs130223
  • Peer Reviewed / Open Access
• [Journal Article] Biochemicaland immunochemical characterization of two discrete vitellogenin proteins and their derived lipovitellins in the inshore hagfish (Eptatretus burgeri) (2014)
  • Author(s): Nishimiya O, Kunihiro Y, Hiramatsu N, Inagawa H, Todo T, Hara A
  • Journal Title: Zoological Science Volume: 31(4) Issue: 4 Pages: 251-257
  • DOI: 10.2108/zs130234
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Development and partial characterization of an antiserum against apolipoprotein B of the short-finned eel, Anguilla australis. (2014)
  • Author(s): Damsteegt, E.L., Mizuta, H., Ozaki, Y., Hiramatsu, N., Todo, T., Hara, A., Ijiri, S., Adachi, S., Lokman, P.M.
  • Journal Title: J. Comp. Physiol. Volume: 184B Pages: 589-599
  • Peer Reviewed
• [Presentation] カットスロートトラウト雌の肝臓におけるエストラジオール17β応答性遺伝子の生殖周期に伴う発現変化 (2017)
  • Author(s): 永田淳・莚平裕次・西宮攻・平松尚志・原彰彦・東藤孝
  • Organizer: 平成29年度日本水産学会春季大会
  • Place of Presentation: 東京海洋大学品川キャンパス（東京都港区）
  • Year and Date: 2017-03-26
• [Presentation] カットスロートトラウト（Oncorhynchus clarki）における2型ビテロジェニンプロモーターの性状解析 (2016)
  • Author(s): 莚平裕次・西宮攻・永田淳・東藤孝・原彰彦・平松尚志
  • Organizer: 平成28年度日本水産学会春季大会
  • Place of Presentation: 東京海洋大学品川キャンパス（東京都港区）
  • Year and Date: 2016-03-26
• [Presentation] Ovarian yolk formation in fishes: molecular mechanisms underlying formation of lipid droplets and vitellogenin-derived yolk proteins. (2014)
  • Author(s): Hiramatsu, N., Todo, T., Sullivan, C., Svilling, J., Reading, B.J., Matsubara, T., Ryu, Y.-W., Mizuta, H., Luo, W., Nishimiya, O., Wu, M., Mushirobira, Y., Yilmaz, O., Hara, A.
  • Organizer: 10th International Symposium on Reproductive Physiology of Fish
  • Place of Presentation: Olhao, Portugal
  • Year and Date: 2014-05-25 – 2014-05-30
  • Invited
• [Presentation] Very low-density lipoprotein is primary carrier of neutral lipids to opossums lipid droplets in teleosts. (2014)
  • Author(s): Ryu, Y.-W., Todo, T., Hiramatsu, N., Matsubara, T., Sullivan, C.V., Hara, A.
  • Organizer: 10th International Symposium on Reproductive Physiology of Fish
  • Place of Presentation: Olhao, Portugal
  • Year and Date: 2014-05-25 – 2014-05-30
  • Invited
• [Presentation] Ligand binding properties of ovarian lipoprotein receptors in the cutthroat trout. (2014)
  • Author(s): Mushirobira, Y., Mizuta, H., Luo, W., Todo, T., Hara, A., Reading, B.J., Sullivan, C.V., Hiramatsu, N.
  • Organizer: 10th International Symposium on Reproductive Physiology of Fish
  • Place of Presentation: Olhao, Portugal
  • Year and Date: 2014-05-25 – 2014-05-30