| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
Three DUR3-like genes (PyDUR3.1/3.2/3.3) with different functional properties, which may be involved in uptake and transport of urea and play important roles in nitrogen metabolism in Pyropia yezoensis (Rhodophyta), have been identified. In this study, expression characteristics of PyDUR3.1/3.2/3.3 depending on the life history phase as well as the nutrient conditions, and partial nucleotide sequences of the upstream regions associated with the regulation of their expression were clarified. Although urea, amino acid, and nucleic acid metabolic pathways in P. yezoensis thalli were greatly influenced by nutrient starvation, each metabolism was restored by chemical fertilization using urea. These results indicate that some metabolites involved in the metabolic pathways mentioned above, are effective in evaluating physiological condition of P. yezoensis thalli.
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