| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
To understand potential of mucosal tissues (gills and intestine) as vaccination sites for inducing adaptive systemic immunity, we investigated T-cell immunity following direct gill infection method, which exposes virus only to gills and anal intubation. Gene expression analysis and flow cytometry analysis showed that vaccination with viral antigen to the mucosal tissues can induced virus-specific T cell immunity in both systemic (kidney) and mucosal (gill and intestine) organs. In addition, the mucosal vaccination could induce specific cell-mediated cytotoxicity and secretion of CHNV-specific IgM in serum, indicating that local priming of the gill site can generate adaptive systemic immunity. Interestingly, intestinal vaccination with inactive virus could induce Th-1 immune response, and provide efficient protection against the virus infection. These findings suggest that the gills and intestine could be prospective antigen-sensitization sites for mucosal vaccination.
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