| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
Gonadotropin-releasing hormone (GnRH) is known to regulate and maintain reproductive functions. Recently, new GnRH has been purified from the American crayfish Procambarus clarkii and its primary structure has been determined. This was the first GnRH from crustacean species. In order to find out the kuruma prawn Marsupenaeus japonicus GnRH, we conducted RNA-seq analysis in this study. Although a homology search using the amino acid sequence of P. clarkii GnRH as a query was conducted, any homologs were not identified in the contigs. On the other hand, three molecules which are members of GnRH superfamily were found out. The three molecules were adipokinetic hormone (AKH)/red pigment concentrating hormone (RPCH), corazonin (Crz), and AKH/corazonin-related peptides (ACP). The three peptides were chemically synthesized and injected into female shrimps. As a result, vitellogenic oocytes were observed in two out of 11 shrimps. Therefore, any of the three peptides may be M. japonicus GnRH.
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