Application of supercooled water in W/O emulsion prepared by microchannel for cryopreservation

• Project/Area Number: 26450364
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Agricultural environmental engineering/Agricultural information engineering
• Research Institution: Gifu University
• Principal Investigator: Iwamoto Satoshi 岐阜大学, 応用生物科学部, 教授 (00373233)
• Co-Investigator(Renkei-kenkyūsha): NAKAGAWA Tomoyuki 岐阜大学, 応用生物科学部, 教授 (70318179)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2014: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
• Keywords: エマルション / 微細水滴 / 過冷却 / 微細液滴 / マイクロチャネル乳化

Outline of Final Research Achievements

We succeeded in microencapsulation to water droplets of budding yeasts in a high including rate. It is easy to predict the crystallization temperature for W/O emulsions with narrow size distribution. This method is not inferior in cell viability as compared with a conventional one.

Research Products

• [Journal Article] MICROENCAPSULATION: A REVIEW OF APPLICATIONS IN THE FOOD AND PHARMACEUTICAL INDUSTRIES (2016)
  • Author(s): Peanparkdee, M., Iwamoto, S. and Yamauchi, R
  • Journal Title: Reviews in Agricultural Science Volume: 4 Issue: 0 Pages: 56-65
  • DOI: 10.7831/ras.4.56
  • NAID: 130007627486
  • ISSN: 2187-090X
  • Peer Reviewed / Open Access
• [Presentation] Encapsulation of extract from Thai riceberry bran using gelatin (2016)
  • Author(s): Peanparkdee, M., Iwamoto, S. and Yamauchi, R.
  • Organizer: 24th International Conference on Bioencapsulation.
  • Place of Presentation: Lisbon, Portugal
  • Year and Date: 2016-09-21
  • Int'l Joint Research
• [Presentation] Non-isothermal crystallization behaviors of supercooled water in monodisperse W/O emulsion (2016)
  • Author(s): T. Nishizaki, K. Yamane, S. Iwamoto, M.Peanparkdee, Y. Teramoto, R. Yamauchi
  • Organizer: The 18th International Conference on Crystal Growth and Epitaxy
  • Place of Presentation: Nagoya, Japan
  • Year and Date: 2016-08-07
  • Int'l Joint Research
• [Presentation] 微細水滴に内包した出芽酵母の生存率向上のための連続相の検討 (2015)
  • Author(s): 山根 克己, 岩本 悟志, 中川 智行, 山内 亮
  • Organizer: 題67回日本生物工学会大会（2015）
  • Place of Presentation: 城山観光ホテル（鹿児島市）
  • Year and Date: 2015-10-26
• [Presentation] APPLICATION OF SUPERCOOLED WATER IN W/O EMULSION PREPARED BY MICROCHANNEL FOR CRYOPRESERVATION (2015)
  • Author(s): Satoshi IWAMOTO, Katsuki YAMANE, Tomoyuki NAKAGAWA, Ryo YAMAUCHI
  • Organizer: 10th European Congress of Chemical Engineering
  • Place of Presentation: Nice, France
  • Year and Date: 2015-09-27
• [Presentation] OBSERVATION OF MONO DISPERSE W/O EMULSION PREPARED BY MICROCHANNEL AT SUBZERO TEMPERATURE (2015)
  • Author(s): Tetushiko NISHIZAKI, Katsuki YAMANE, Satoshi IWAMOTO, Ryo YAMAUCHI
  • Organizer: 10th European Congress of Chemical Engineering
  • Place of Presentation: Nice, France
  • Year and Date: 2015-09-27
• [Presentation] 微細水滴を用いた微生物の新規な低温保存の検討 (2015)
  • Author(s): 山根 克己,岩本悟志,中川 智行,山内 亮
  • Organizer: 日本化学会第95春季年会
  • Place of Presentation: 日本大学 船橋キャンパス
  • Year and Date: 2015-03-26 – 2015-03-29
• [Presentation] 過冷却現象を利用した微生物の低温保存の検討 (2014)
  • Author(s): 山根 克己,岩本悟志,中川 智行,山内 亮
  • Organizer: 日本農芸化学会中部支部 第171例会
  • Place of Presentation: 名古屋大学
  • Year and Date: 2014-10-11
• [Presentation] 微細水滴を用いた微生物の新規な低温保存の検討 (2014)
  • Author(s): 山根 克己,岩本悟志,中川 智行,山内 亮
  • Organizer: 第66回 日本生物工学会大会
  • Place of Presentation: 北海道コンベンションセンター
  • Year and Date: 2014-09-07 – 2014-09-11