Involvement of nuclear and mitochondrial genome on immune function and reproductive performance in dairy cow

• Project/Area Number: 26450372
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Animal production science
• Research Institution: Obihiro University of Agriculture and Veterinary Medicine
• Principal Investigator: SHIMIZU TAKASHI 帯広畜産大学, 畜産学部, 准教授 (90375113)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 繁殖性 / 免疫機能 / 一塩基多型 / ゲノム / ミトコンドリアDNA / 乳牛 / 免疫細胞機能 / 細菌感染 / ミトコンドリア / 免疫細胞

Outline of Final Research Achievements

Toll-like receptor 4 (TLR4) and TLR2 are receptor for LPS that is released from gram-negative bacteria. In postpartum dairy cows, inflammation diseases such as metritis and mastitis suppress reproductive performance. The present study is to examine the relationships among SNPs for TLR4 and TLR2 and reproductive performance and immune functions. TLR4 SNP affect the production of cytokine, apoptosis and migration of immune cells. In addition, TLR4 SNP affect ovarian function such as ovulation. Moreover, TLR2 SNP influence follicular cell function (steroid production). These data suggest that SNPs for TLR4 and TLR2 are associated with reproductive performance and immune function.

Research Products

• [Journal Article] Impact of metritis on the generation of reactive oxygen species by circulating phagocytes and plasma lipopolysaccharide concentration in peripartum dairy cows. (2017)
  • Author(s): Magata F, Morino I, Teramura M, Tsunoda E, Kawashima C, Haneda S, Miyamoto A, Kida K, Shimizu T.
  • Journal Title: Anim. Sci. J. Volume: 88 Issue: 2 Pages: 248-253
  • DOI: 10.1111/asj.12642
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Effect of Single Nucleotide Polymorphisms of Toll-Like Receptor 4 (TLR 4) on Reproductive Performance and Immune Function in Dairy Cows. (2017)
  • Author(s): Shimizu T, Kawasaki Y, Aoki Y, Magata F, Kawashima C, Miyamoto A.
  • Journal Title: Biochem. Genet. Volume: 印刷中 Issue: 3 Pages: 212-222
  • DOI: 10.1007/s10528-017-9790-0
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Effect of lipopolysaccharide on progesterone production during luteinization of granulosa and theca cells in vitro. (2016)
  • Author(s): Shimizu T., Echizenya R., Miyamoto A.
  • Journal Title: J. Biochem. Mol. Toxicol. Volume: 30 Issue: 4 Pages: 206-211
  • DOI: 10.1002/jbt.21783
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Long-term impact of puerperal metritis on the profiles of peripheral blood leukocytes in peripartum dairy cows. (2016)
  • Author(s): Magata, F., Kitaoka, R., Morino, I., Teramura, M., Kawashima, C., Haneda, S., Shimizu, T.
  • Journal Title: Anim. Sci. J. Volume: 87 Issue: 1 Pages: 151-155
  • DOI: 10.1111/asj.12516
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Comparison of bacterial endotoxin lipopolysaccharide concentrations in the blood, ovarian follicular fluid and uterine fluid: a clinical case of bovine metritis (2015)
  • Author(s): Magata F, Ishida Y, Miyamoto A, Furuoka H, Inokuma H, Shimizu T
  • Journal Title: Journal of Veterinary Medical Science Volume: 77 Issue: 1 Pages: 81-84
  • DOI: 10.1292/jvms.14-0333
  • NAID: 130004690850
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Effect in dedicator of cytokinesis 6 (DOCK6) on steroid production in theca cells of follicular cysts. (2015)
  • Author(s): Murayama, C., Yamasaki, E., Miyamoto, A., Shimizu, T.
  • Journal Title: Biochem. Biophys. Res. Commun. Volume: 462 Issue: 4 Pages: 415-419
  • DOI: 10.1016/j.bbrc.2015.05.005
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] The effect of single nucleotide polymorphisms in the tumor necrosis factor-a gene on reproductive performance and immune function in dairy cattle. (2014)
  • Author(s): Kawasaki, Y., Aoki, Y., Magata, F., Miyamoto, A., Kawashima, C., Hojo, T., Okuda, K., Shirasuna, K., Shimizu, T.
  • Journal Title: J. Reprod. Dev. Volume: 60 Pages: 173-178
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Lipopolysaccharide (LPS) inhibits steroid production in theca cells of bovine follicles: Distinct effect of LPS on theca cell function in pre- and post-selection follicles. (2014)
  • Author(s): Magata, F., Horiuchi, M., Miyamoto, A., Shimizu, T.
  • Journal Title: J. Reprod. Dev. Volume: 60 Pages: 280-287
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Lipopolysaccharide in ovarian follicular fluid influences the steroid production in large follicles of dairy cows (2014)
  • Author(s): Magata F, Horiuchi M, Echizenya R, Miura R, Chiba S, Matsui M, Miyamoto A, Kobayashi Y, Shimizu T
  • Journal Title: Animal Reproduction Science Volume: 144(1-2) Issue: 1-2 Pages: 6-13
  • DOI: 10.1016/j.anireprosci.2013.11.005
  • Peer Reviewed / Open Access
• [Presentation] Effect of endotoxin on ovarian follicle function in domestic animals. (2015)
  • Author(s): Shimizu, T., Horiuchi, M., Miyauchi K., Echizenya, R., Magata, F., Miyamoto, A.
  • Organizer: International Conference on Biology and Pathology of Reproduction in Domestic Animals
  • Place of Presentation: Gdansk, Poland
  • Year and Date: 2015-09-26
  • Int'l Joint Research / Invited