Improving meat production by fundamental research on myogenic differentiation

• Project/Area Number: 26450391
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Animal production science
• Research Institution: Kyushu University
• Principal Investigator: Nakamura Mako 九州大学, 農学研究院, 准教授 (80452746)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2016: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2015: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 筋細胞分化 / 食肉科学 / 畜産物 / 活性化衛星細胞 / emerin / Pax7 / Semaphorin 3A / Sema3A / Myf5 / MyoD / semaphorin 3a / differentiation

Outline of Final Research Achievements

Muscle regeneration is initiated by activation of muscle stem cells, known as satellite cells, which are adjacent to the basal lamina around the proximal region of each myofiber.We previously showed that Semaphorin 3A (Sema3A) expression was induced when quiescent muscle became activated satellite cells (ASCs). However, how Sema3A regulates genes in the early phase of ASCs remains unclear. In this study, we investigated whether Sema3A signaling can regulate the early phase of ASCs. We showed that expression of the myogenic proliferation regulatory factors Pax7 and Myf5 was decreased in myoblasts transfected with Sema3A siRNA. Emerin was also regulated by Sema3A in ASCs.

Research Products

• [Journal Article] Mouse soleus (slow) and muscle shows greater intramyocellular lipid droplet accumulateon than EDL (fast) muscle: fiber type-specific analysis (2017)
  • Author(s): Komiya Y, Sawano S, Mashima D, Ichitsubo R, Nakamura M, Tatsumi R, Ikeuchi Y, Mizunoya W
  • Journal Title: Journal of Muscle Research and Cell Motility Volume: 印刷中 Issue: 2 Pages: 163-173
  • DOI: 10.1007/s10974-017-9468-6
  • Peer Reviewed / Open Access
• [Journal Article] APOBEC2 negatively regulates myoblast differentiation in muscle regeneration (2017)
  • Author(s): Hideaki Ohtsubo, Yusuke Sato, Takahiro Suzuki, Wataru Mizunoya, Mako Nakamura, Ryuichi Tatsumi, Yoshihide Ikeuchi
  • Journal Title: International Journal of Biochemistry and Cell Biology Volume: 85 Pages: 91-101
  • DOI: 10.1016/j.biocel.2017.02.005
  • Peer Reviewed
• [Journal Article] A One-Step Immunostaining Method to Visualize Rodent Muscle Fiber Type within a Single Specimen. (2016)
  • Author(s): Sawano S, Komiya Y, Ichitsubo R, Ohkawa Y, Nakamura M, Tatsumi R, Ikeuchi Y, Mizunoya W.
  • Journal Title: PLoS One. Volume: 11 Issue: 11 Pages: e0166080-e0166080
  • DOI: 10.1371/journal.pone.0166080
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Protocol for rat single muscle-fiber isolation and culture (2015)
  • Author(s): Yusuke Komiya, Judy E. Anderson, Mariko Akahoshi, Mako Nakamura, Ryuichi Tatsumi, Yoshihide Ikeuchi, Wataru Mizunoya
  • Journal Title: Analytical Biochemistry Volume: 482 Pages: 22-24
  • DOI: 10.1016/j.ab.2015.03.034
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Data in support for protocol for rat single muscle-fiber isolation and culture (2015)
  • Author(s): Yusuke Komiya, Judy E. Anderson, Mariko Akahoshi, Mako Nakamura, Ryuichi Tatsumi, Yoshihide Ikeuchi, Wataru Mizunoya
  • Journal Title: Data in Brief Volume: 4 Pages: 7-10
  • DOI: 10.1016/j.dib.2015.04.016
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Transmembrane proteoglycans syndecan-2, 4, receptor candidates for the impact of HGF and FGF2 on semaphorin 3A expression in early-differentiated myoblasts (2015)
  • Author(s): Mai-Khoi Q. Do, Naomi Shimizu, Takahiro Suzuki, Hideaki Ohtsubo, Wataru Mizunoya, Mako Nakamura, Shoko Sawano, Mitsuhiro Furuse, Yoshihide Ikeuchi, Judy E. Anderson, Ryuichi Tatsumi
  • Journal Title: Physiological Reports Volume: 3 Issue: 9 Pages: e12553-e12553
  • DOI: 10.14814/phy2.12553
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Semaphorin 3A promotes activation of Pax7, Myf5, and MyoD through inhibition of emerin expression in activated satellite cells (2015)
  • Author(s): Mulan Qahar, Yuko Takuma, Wataru Mizunoya, Ryuichi Tatsumi, Yoshihide Ikeuchi, Mako Nakamura
  • Journal Title: FEBS openbio Volume: - Issue: 6 Pages: 529-539
  • DOI: 10.1002/2211-5463.12050
  • Peer Reviewed / Open Access
• [Journal Article] 筋肥大・再生における異種細胞間コミュニケーションダイナミクス ―筋幹細胞による運動神経支配の再構築制御(仮説)を話題にして― (2015)
  • Author(s): 辰巳 隆一, Do Mai-Khoi Quy, 鈴木貴弘, 大屋雄暉, 大坪秀明, 小宮佑介, 澤野祥子, 水野谷 航, 中村 真子, 池内 義秀
  • Journal Title: 食肉の科学（日本食肉研究会） Volume: 56 Pages: 103-112
• [Journal Article] Implication of anti-inflammatory macrophages in regenerative moto-neuritogenesis: Promotion of myoblast migration and neural chemorepellent semaphorin 3A expression in injured muscle. (2014)
  • Author(s): Sakaguchi S, Shono J, Suzuki T, Shoko S, Anderson JE, Do MKQ, Ohtsubo H, Mizunoya W, Sato Y, Nakamura M, Furuse M, Yamada K, Ikeuchi Y, Tatsumi R.
  • Journal Title: International Journal of Biochemistry and Cell Biology Volume: 52 Pages: 272-285
  • DOI: 10.1016/j.biocel.2014.05.032
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] 筋幹細胞が分泌するSema3Aによる筋線維型自律制御機構 (2014)
  • Author(s): 鈴木 貴弘, 大屋 雄暉, 尾嶋 孝一, 大坪 秀明, 小宮 佑介, 水野谷 航, 中村 真子, 池内 義秀, 古瀬 充宏, 辰巳 隆一
  • Journal Title: 栄養生理研究会報（家畜栄養生理研究会） Volume: 58 Pages: 19-25
  • NAID: 40020020774
  • Peer Reviewed
• [Presentation] 放牧飼育による習慣的運動負荷が豚肉の肉質に与える効果 (2017)
  • Author(s): 大座啓史, 村上徹哉, 笠正二郎, 間島大介, 中村 真子, 辰巳 隆一, 水野谷 航
  • Organizer: 日本畜産学会第122回大会
  • Place of Presentation: 神戸大学（兵庫県・神戸市）
  • Year and Date: 2017-03-27
• [Presentation] 長期筋萎縮で発現変動する遺伝子の網羅的探索 (2017)
  • Author(s): 福島美咲, 赤坂泰輝, 澤野祥子, 水野谷 航, 辰巳 隆一, 中村 真子, 池内 義秀
  • Organizer: 日本畜産学会第122回大会
  • Place of Presentation: 神戸大学（兵庫県・神戸市）
  • Year and Date: 2017-03-27
• [Presentation] Semaphorin 3Aによる遅筋型筋線維形成機構の解明 (2017)
  • Author(s): 柴田歩実, 川口舞, 水野谷 航, 中村 真子, 池内 義秀, 辰巳 隆一
  • Organizer: 日本畜産学会第122回大会
  • Place of Presentation: 神戸大学（兵庫県・神戸市）
  • Year and Date: 2017-03-27
• [Presentation] ユキレイタケの子実体および菌床中に含まれるPPARδ活性化成分の探索 (2017)
  • Author(s): 井本大輔, 西條裕美, 清水 邦義, 中村 真子, 辰巳 隆一, 水野谷 航
  • Organizer: 日本畜産学会第122回大会
  • Place of Presentation: 神戸大学（兵庫県・神戸市）
  • Year and Date: 2017-03-27
• [Presentation] Role for semaphorin 3A in regulation of myogenic stem cells proliferation (2016)
  • Author(s): Mulan Qahar, Wataru Mizunoya, Ryuichi Tatsumi, Mako Nakamura
  • Organizer: The 17th Asian-Australasian Association of Animal Production Societies Animal Science Congress
  • Place of Presentation: Kyushu Sangyo University （福岡県・福岡市）
  • Year and Date: 2016-08-22
  • Int'l Joint Research
• [Presentation] Yukireitake mushroom(P. eryngii var. tuoliensis CJ.Mou)has both agonistic and antagonistic activities against peroxisome proliferator-activated receptor(PPAR)delta (2016)
  • Author(s): Wataru Mizunoya, Daisuke Imoto, Mako Nakamura, Ryuichi Tatsumi, Yoshihide Ikeuchi
  • Organizer: The 17th Asian-Australasian Association of Animal Production Societies Animal Science Congress
  • Place of Presentation: Kyushu Sangyo University （福岡県・福岡市）
  • Year and Date: 2016-08-22
  • Int'l Joint Research
• [Presentation] Muscle fiber type visualization using four anti-myosin heavy chain antibodies (2016)
  • Author(s): Shoko Sawano, Yusuke Komiya, Riho Ichitsubo, Mako Nakamura, Ryuichi Tatsumi, Yoshihide Ikeuchi, Wataru Mizunoya
  • Organizer: The 17th Asian-Australasian Association of Animal Production Societies Animal Science Congress
  • Place of Presentation: Kyushu Sangyo University （福岡県・福岡市）
  • Year and Date: 2016-08-22
  • Int'l Joint Research
• [Presentation] Sema3Aはemerin 発現を阻害することで筋特異的因子Pax7およびMyf5 発現を増加させ、筋芽細胞の増殖能を上昇させる (2016)
  • Author(s): Mulann Qahar, Wataru Mizunoya, Ryuichi Tatsumi, Yoshihide Ikeuchi, Mako Nakamura
  • Organizer: Molecular Mechanisms Modulating Skeletal Muscle Development and Homeostasis in Health and Disease
  • Place of Presentation: Asilomar Conference Grounds, Pacific Grove （United States of America）
  • Year and Date: 2016-06-09
  • Int'l Joint Research
• [Presentation] エイコサペンタエン酸はラット骨格筋の脂肪代謝関連遺伝子発現を増加させる (2016)
  • Author(s): 小宮佑介, 中村 真子, 辰巳 隆一, 池内 義秀, 水野谷 航
  • Organizer: 第70回日本栄養・食糧学会大会
  • Place of Presentation: 武庫川女子大学中央キャンパス（兵庫県・西宮市）
  • Year and Date: 2016-05-13
• [Presentation] 4種類の新規ミオシン重鎖抗体を用いた筋線維タイプ同定法 (2016)
  • Author(s): 水野谷 航, 小宮佑介, 澤野 祥子, 中村 真子, 辰巳 隆一, 池内 義秀
  • Organizer: 第70回日本栄養・食糧学会大会
  • Place of Presentation: 武庫川女子大学中央キャンパス（兵庫県・西宮市）
  • Year and Date: 2016-05-13
• [Presentation] Sema3Aはemerin 発現を阻害することで筋特異的因子Pax7およびMyf5 発現を増加させ、筋芽細胞の増殖能を上昇させる (2016)
  • Author(s): 木蘭, 水野谷 航, 辰巳 隆一, 池内 義秀, 中村 真子
  • Organizer: 第121回日本畜産学会大会
  • Place of Presentation: 日本獣医生命科学大学、武蔵野市
  • Year and Date: 2016-03-27
• [Presentation] エイコサペンタエン酸投与はラット骨格筋の脂肪代謝関連遺伝子発現を増加させる (2016)
  • Author(s): 小宮佑介, 中村 真子, 辰巳 隆一, 池内 義秀, 水野谷 航
  • Organizer: 第121回日本畜産学会大会
  • Place of Presentation: 日本獣医生命科学大学、武蔵野市
  • Year and Date: 2016-03-27
• [Presentation] 骨格筋形成における脱アミノ化酵素APOBEC2 の機能 (2016)
  • Author(s): 大坪 秀明, 佐藤 祐介, 鈴木 貴弘, 水野谷 航, 中村 真子, 辰巳 隆一, 池内 義秀
  • Organizer: 第121回日本畜産学会大会
  • Place of Presentation: 日本獣医生命科学大学、武蔵野市
  • Year and Date: 2016-03-27
• [Presentation] Pax7 が筋細胞および脂肪細胞の分化に与える影響 (2016)
  • Author(s): 詫間 裕子, 木蘭, 水野谷 航, 辰巳 隆一, 池内 義秀, 中村 真子
  • Organizer: 第121回日本畜産学会大会
  • Place of Presentation: 日本獣医生命科学大学、武蔵野市
  • Year and Date: 2016-03-27
• [Presentation] マウス遅筋及び速筋を用いた代謝物プロファイリング (2015)
  • Author(s): 澤野祥子, 中村 真子, 辰巳 隆一, 池内 義秀, 水野谷 航
  • Organizer: 第38回日本分子生物学会年会 第88回日本生化学会大会合同大会
  • Place of Presentation: 神戸ポートアイランド、神戸市
  • Year and Date: 2015-12-01
• [Presentation] Sema3Aによる筋線維型の初期決定に関する研究 (2015)
  • Author(s): 大屋雄暉, 鈴木貴弘, Do Mai-Khoi, 水野谷 航, 中村 真子, 池内 義秀, 辰巳 隆一
  • Organizer: 日本畜産学会第120回大会
  • Place of Presentation: 酪農学園大学（江別市）
  • Year and Date: 2015-09-11
• [Presentation] エイコサペンタエン酸はラット単離筋線維の脂肪代謝に関わるタンパク質発現を上昇させる (2015)
  • Author(s): 小宮佑介, 中村 真子, 辰巳 隆一, 池内 義秀, 水野谷 航
  • Organizer: 日本畜産学会第120回大会
  • Place of Presentation: 酪農学園大学（江別市）
  • Year and Date: 2015-09-11
• [Presentation] 筋芽細胞が合成・分泌するSema3Aはアセチルコリン受容体の発現と機能的凝集を制御する (2015)
  • Author(s): Mai-Khoi Q. Do, 水野谷 航, 尾嶋孝一, 中村 真子, 池内 義秀, Judy E. Anderson, 辰巳 隆一
  • Organizer: 第1回日本筋学会学術集会
  • Place of Presentation: 国立研究開発法人国立精神・神経医療研究センター（小平市）
  • Year and Date: 2015-08-08
• [Presentation] 筋芽細胞におけるSema3AとPax7発現の相互作用に関する研究 (2015)
  • Author(s): 木蘭, 尾嶋孝一, 大川 恭行, 中村 真子, 水野谷 航, 池内 義秀, 辰巳 隆一
  • Organizer: 第1回日本筋学会学術集会
  • Place of Presentation: 国立研究開発法人国立精神・神経医療研究センター（小平市）
  • Year and Date: 2015-08-08
• [Presentation] Effect of Yamabushitake mushroom (Hericium erinaceus) intake on functional and physicochemical properties of skeletal muscle in mice (2015)
  • Author(s): Wataru Mizunoya, Toshiya Nakamura, Daisuke Imoto, Yusuke Komiya, Fuminori Kawabata, Mako Nakamura, Ryuichi Tatsumi, Yoshihide Ikeuchi
  • Organizer: ACN2015(12th Asian Congress of Nutrition)
  • Place of Presentation: PACIFICO Yokohama
  • Year and Date: 2015-05-14
  • Int'l Joint Research
• [Presentation] Eicosapentaenoic acid in fish oil is the key fatty acid to promote oxidative metabolism in rat skeletal muscle (2015)
  • Author(s): Yusuke Komiya, Tsuyoshi Goto, Nobuyuki Takahashi, Teruo Kawada, Mako Nakamura, Ryuichi Tatsumi, Yoshihide Ikeuchi, Wataru Mizunoya
  • Organizer: ACN2015(12th Asian Congress of Nutrition)
  • Place of Presentation: PACIFICO Yokohama
  • Year and Date: 2015-05-14
  • Int'l Joint Research
• [Presentation] Transcriptomic and metabolomic analyses depicted distinct characteristics between slow-twitch and fast-twitch muscles in mice (2015)
  • Author(s): Shoko Sawano, Yuri Tajima, Johan Rung, Mako Nakamura, Ryuichi Tatsumi, Yoshihide Ikeuchi, Wataru Mizunoya
  • Organizer: ACN2015(12th Asian Congress of Nutrition)
  • Place of Presentation: PACIFICO Yokohama
  • Year and Date: 2015-05-14
  • Int'l Joint Research
• [Presentation] 筋芽細胞の増殖時・分化時におけるSema3AとPax7発現の相互作用 (2015)
  • Author(s): 木 蘭, 尾嶋孝一, 中村真子, 水野谷航, 辰巳隆一, 池内義秀
  • Organizer: 第119回日本畜産学会大会
  • Place of Presentation: 宇都宮大学
  • Year and Date: 2015-03-27 – 2015-03-30
• [Presentation] Sema3A ligand secreted from satellite cells promotes aneural nAChR clustering (2015)
  • Author(s): ド マイコイ, 水野谷航, 尾嶋孝一, 中村真子, 池内義秀, 辰巳隆一
  • Organizer: 第119回日本畜産学会大会
  • Place of Presentation: 宇都宮大学
  • Year and Date: 2015-03-27 – 2015-03-30
• [Presentation] 遅筋は速筋に比べ脂肪酸取込および脂肪合成能に優れる (2015)
  • Author(s): 小宮佑介, 澤野祥子, 田嶋悠璃, Johan Rung, 辰巳隆一, 中村真子, 池内義秀, 水野谷航
  • Organizer: 第119回日本畜産学会大会
  • Place of Presentation: 宇都宮大学
  • Year and Date: 2015-03-27 – 2015-03-30
• [Presentation] PPARδアゴニスト投与によってマウス骨格筋で発現変動する遺伝子の網羅的解析 (2015)
  • Author(s): 田嶋悠璃, Johan Rung, 澤野祥子, 小宮佑介, 中村真子, 辰巳隆一,池内義秀
  • Organizer: 第119回日本畜産学会大会
  • Place of Presentation: 宇都宮大学
  • Year and Date: 2015-03-27 – 2015-03-30
• [Presentation] １切片上で筋線維タイプを同時に識別する“ステンドグラス染色法”の開発 (2014)
  • Author(s): 澤野祥子, 小宮佑介, 中村真子, 辰巳隆一, 池内義秀, 水野谷航
  • Organizer: 第37回(平成26年度)日本分子生物学会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2014-11-25 – 2014-11-27
• [Presentation] 筋幹細胞由来の分泌性因子Sema3Aによる筋線維型自律制御機構 (2014)
  • Author(s): 鈴木貴弘, 大屋雄暉, 澤野祥子, 大坪秀明, 水野谷航, 中村真子, 池内義秀, 辰巳隆一
  • Organizer: 第37回(平成26年度)日本分子生物学会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2014-11-25 – 2014-11-27
• [Presentation] Anti-inflammatory macrophages implicate in regenerative moto-neuritogenesis, by promoting myoblast migration and Sema3A expression. (2014)
  • Author(s): Sakaguchi, S.,* Shono, J.,* Suzuki, T.,* Sawano, S., Anderson, J.E., Do, M.-K.Q., Ohtsubo, H., Mizunoya, W., Nakamura, M., Furuse, M., Ikeuchi, Y., and Tatsumi, R. (*equal contributors)
  • Organizer: Animal Science Congress 2014 of the Asian-Australian Association of Animal Production Societies
  • Place of Presentation: Universitas Gadjah Mada (UGM),Indonesia
  • Year and Date: 2014-11-10 – 2014-11-14
• [Presentation] Functional interaction between Sema3A and Pax7 in proliferation and differentiation of myoblasts. (2014)
  • Author(s): Mulan, Nakamura, M., Suzuki, T., Mizunoya, W., Ojima, K., Tatsumi, R. and Ikeuchi, Y.
  • Organizer: International Symposium on Agriculture, Forestry, Environment and Life Sciences in Asia, 2014 (AFELiSA'14)
  • Place of Presentation: Ladena Condominium, Korea
  • Year and Date: 2014-10-28 – 2014-10-31
• [Presentation] M2 macrophages may implicate in regenerative moto-neuritogenesis, by promoting myoblast migration and Sema3A expression. (2014)
  • Author(s): Tatsumi, R.,* Sakaguchi, S.,* Shono, J.,* Suzuki, T.,* Sawano, S., Anderson, J.E., Do, M.-K.Q., Ohtsubo, H., Mizunoya, W., Nakamura, M., Furuse, M., and Ikeuchi, Y. (*equal contributors)
  • Organizer: 2014 FASEB Science Research Conference on “Skeletal Muscle Satellite and Stem Cells”
  • Place of Presentation: Steamboat Springs, CO, USA
  • Year and Date: 2014-07-20 – 2014-07-25
• [Presentation] APOBEC2 deficiency up-regulates myoblast differentiation. (2014)
  • Author(s): Ohtsubo, H., Sato, Y., Suzuki, T., Mizunoya, W., Nakamura, M., Tatsumi, R., and Ikeuchi, Y.
  • Organizer: 2014 FASEB Science Research Conference on “Skeletal Muscle Satellite and Stem Cells”
  • Place of Presentation: Steamboat Springs, CO, USA
  • Year and Date: 2014-07-20 – 2014-07-25
• [Presentation] Sema3A secreted from satellite cells promotes slow-twitch fiber generation. (2014)
  • Author(s): Suzuki, T., Ohya, Y., Ojima, K., Mizunoya, W., Sawano, S., Ohtsubo, H., Nishimatsu, S., Anderson, J. E., Do, M.-K. Q., Nakamura, M., Furuse, M., Ikeuchi, Y., Nohno, T., and Tatsumi, R.
  • Organizer: 2014 FASEB Science Research Conference on “Skeletal Muscle Satellite and Stem Cells”
  • Place of Presentation: Steamboat Springs, CO, USA
  • Year and Date: 2014-07-20 – 2014-07-25
• [Presentation] ヤマブシタケは骨格筋の核内受容体PPARδを活性化する (2014)
  • Author(s): 水野谷航, 石井もも子, 清水邦義, 永田敏郎, 平木絵里, 中村俊哉, 中村真子, 辰巳隆一, 池内義秀
  • Organizer: 第68回日本栄養・食糧学会大会
  • Place of Presentation: 酪農学園大学
  • Year and Date: 2014-06-01
• [Presentation] 筋幹細胞が分泌するSema3Aによる筋線維型自律制御機構 (2014)
  • Author(s): 鈴木貴弘, 大屋雄暉, 尾嶋孝一, 大坪秀明, 小宮佑介, 水野谷航, 中村真子, 池内義秀, 古瀬充宏, 辰巳隆一
  • Organizer: 平成26年度家畜栄養生理研究会春季集談会
  • Place of Presentation: 日本獣医生命科学大学
  • Year and Date: 2014-05-17