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Analysis of supressive role of complement factors in prion propagation

Research Project

Project/Area Number 26450397
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Veterinary medical science
Research InstitutionHokkaido University

Principal Investigator

Hasebe Rie  北海道大学, 獣医学研究院, 講師 (70431335)

Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywordsプリオン病 / 補体因子 / 神経病態
Outline of Final Research Achievements

Roles of complement factors in neuropathogenesis of prion diseases are unknown. The results of the current study suggest that the amount of abnormal form of prion protein (PrPSc) was reduced in Chandler-infected primary cultured neurons, but was increased in 22L-infected neurons after temporary decrease. To assess the effects of complement reaction in in vivo prion infection, I injected lentivirus vectors harboring shRNA against complement factors C1qA and C3 into prion-infected mouse brains. In Chandler-infected mice, PrPSc was increased after C1q- and C3-shRNA injection. In contrast, PrPSc was decreased in 22L-infected mouse brains after C3-shRNA injection, but not C1qA-shRNA injection. These results suggest that the type and the effect of complement factors on the neuropathogenesis were different among the prion strains

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (33 results)

All 2016 2015 2014

All Journal Article (7 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 7 results,  Acknowledgement Compliant: 5 results,  Open Access: 2 results) Presentation (26 results) (of which Int'l Joint Research: 8 results)

  • [Journal Article] Complement factors alter the amount of PrP(Sc) in primary-cultured mouse cortical neurons associated with increased membrane permeability2016

    • Author(s)
      Hasebe R, Tanaka M, Suzuki A, Yamasaki T, Horiuchi M
    • Journal Title

      Virology

      Volume: 496 Pages: 9-20

    • DOI

      10.1016/j.virol.2016.05.014

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Comparison of abnormal isoform of prion protein in prion-infected cell lines and primary-cultured neurons by PrPSc-specific immunostaining2016

    • Author(s)
      Tanaka M, Fujiwara A, Suzuki A, Yamasaki T, Hasebe R, Masujin K, Horiuchi M
    • Journal Title

      J Gen Virol

      Volume: 97 Issue: 8 Pages: 030-2042

    • DOI

      10.1099/jgv.0.000514

    • NAID

      120006331855

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Establishment of a simple cell-based ELISA for the direct detection of abnormal isoform of prion protein from prion-infected cells without cell lysis and proteinase K treatment2016

    • Author(s)
      Shan Z, Yamasaki T, Suzuki A, Hasebe R, Horiuchi M
    • Journal Title

      Prion

      Volume: 10 Issue: 4 Pages: 305-318

    • DOI

      10.1080/19336896.2016.1189053

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Temporary upregulation of anti-inflammatory cytokine IL-13 expression in the brains of CD14 deficient mice in the early stage of prion infection.2014

    • Author(s)
      Hasebe R, Suzuki A, Yamasaki T, Horiuchi M
    • Journal Title

      Biochem Biophys Res Commun

      Volume: 454 Issue: 1 Pages: 125-130

    • DOI

      10.1016/j.bbrc.2014.10.043

    • NAID

      120005540293

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Comparison of the anti-prion mechanism of four different anti-prion compounds, anti-PrP monoclonal antibody 44B1, pentosan polysulfate, chlorpromazine, and U18666A, in prion-infected mouse neuroblastoma cells.2014

    • Author(s)
      Yamasaki T, Suzuki A, Hasebe R, Horiuchi M
    • Journal Title

      PLoS One

      Volume: 9 Issue: 9 Pages: e106516-e106516

    • DOI

      10.1371/journal.pone.0106516

    • NAID

      120005476205

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Autophagy inhibits viral genome replication and gene expression stages in West Nile virus infection.2014

    • Author(s)
      Kobayashi S, Orba Y, Yamaguchi H, Takahashi K, Sasaki M, Hasebe R, Kimura T, Sawa H.
    • Journal Title

      Virus Res

      Volume: 191 Pages: 83-91

    • DOI

      10.1016/j.virusres.2014.07.016

    • NAID

      120005517727

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Tick-borne flaviviruses alter membrane structure and replicate in dendrites of primary mouse neuronal cultures.2014

    • Author(s)
      Minato Hirano, Kentaro Yoshii, Mizuki Sakai, Rie Hasebe, Osamu Ichii and Hiroaki Kariwa
    • Journal Title

      JOURNAL OF GENERAL VIROLOGY

      Volume: 95 Issue: 4 Pages: 849-861

    • DOI

      10.1099/vir.0.061432-0

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Presentation] Neuron and glial cell type-specific detection of PrPSc in prion-infected mouse brain by flow cytometry.2016

    • Author(s)
      Yamasaki T, Suzuki A, Hasebe R, Horiuchi M
    • Organizer
      第64回日本ウイルス学会学術集会
    • Place of Presentation
      北海道、札幌
    • Year and Date
      2016-09-23
    • Related Report
      2016 Annual Research Report
  • [Presentation] Analysis of activation state of microglia and astrocytes in prion-infected mice by RNA-sequencing2016

    • Author(s)
      Kuroda M, Yamasaki T, Hasebe R, Suzuki A, Horiuchi M
    • Organizer
      第64回日本ウイルス学会学術集会
    • Place of Presentation
      北海道、札幌
    • Year and Date
      2016-09-23
    • Related Report
      2016 Annual Research Report
  • [Presentation] Utility of cervid recombinant PrP for the detection of L-BSE and CWD prions by RT-QuIC.2016

    • Author(s)
      Suzuki A, Yamasaki T, Hasebe R, Horiuchi M
    • Organizer
      第64回日本ウイルス学会学術集会
    • Place of Presentation
      北海道、札幌
    • Year and Date
      2016-09-23
    • Related Report
      2016 Annual Research Report
  • [Presentation] 北大農場で発生したリステリア症罹患羊の飼育環境からのリステリア属菌の分離とゲノム解析2016

    • Author(s)
      長谷部 理絵, 青島 圭佑, 棟居 佳子, 三谷 朋弘, 平井 佑治, 山﨑 剛士, 木村 享史, 堀内 基広
    • Organizer
      第159回日本獣医学会学術集会
    • Place of Presentation
      神奈川県、藤沢
    • Year and Date
      2016-09-06
    • Related Report
      2016 Annual Research Report
  • [Presentation] リステリアモノサイトゲネスの腸管上皮細胞モデルとの相互作用解析と分子遺伝学的解析2016

    • Author(s)
      平井 佑治, 神吉 政史, 長谷川 めぐみ, 池田 徹也, 長谷部 理絵, 堀内 基広
    • Organizer
      第159回日本獣医学会学術集会
    • Place of Presentation
      神奈川県、藤沢
    • Year and Date
      2016-09-06
    • Related Report
      2016 Annual Research Report
  • [Presentation] 異常型プリオンタンパク質 (PrPSc) 特異的染色法によるプリオン持続感染株化細胞とプリオン感染初代培養神経細胞におけるPrPScの解析2016

    • Author(s)
      田中 美咲, 舛甚 賢太郎, 山﨑 剛士, 長谷部 理絵, 鈴木 章夫, 堀内 基広
    • Organizer
      第159回日本獣医学会学術集会
    • Place of Presentation
      神奈川県、藤沢
    • Year and Date
      2016-09-06
    • Related Report
      2016 Annual Research Report
  • [Presentation] 次世代シーケンサーを用いた、プリオン感染マウス脳由来ミクログリアおよびアストロサイトの活性化状態の解析2016

    • Author(s)
      黒田 弥乃梨, 山﨑 剛士, 鈴木 章夫, 長谷部 理絵, 堀内 基広
    • Organizer
      第159回日本獣医学会学術集会
    • Place of Presentation
      神奈川県、藤沢
    • Year and Date
      2016-09-06
    • Related Report
      2016 Annual Research Report
  • [Presentation] Neuron and glial cell type-specific detection of PrPSc in prion-infected mouse brain by flow cytometry.2016

    • Author(s)
      Yamasaki T, Suzuki A, Hasebe R, Horiuchi M
    • Organizer
      Prion2016
    • Place of Presentation
      Chiyoda-ku, Tokyo, Japan
    • Year and Date
      2016-05-10
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Transcriptome analysis of microglia and astrocytes in prion-infected mice2016

    • Author(s)
      Kuroda M, Yamasaki T, Suzuki A, Hasebe R , Horiuchi M
    • Organizer
      Prion2016
    • Place of Presentation
      Chiyoda-ku, Tokyo, Japan
    • Year and Date
      2016-05-10
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Comparison of abnormal isoform of prion protein in prion-infected cell lines and primary cultured neurons by PrPSc-specific immunostaining2016

    • Author(s)
      Tanaka M, Fujiwara A, Suzuki A, Yamasaki T, Hasebe R, Masujin K, Horiuchi M
    • Organizer
      Prion2016
    • Place of Presentation
      Chiyoda-ku, Tokyo, Japan
    • Year and Date
      2016-05-10
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Comparison of PrPSc-specific staining with two anti-PrP monoclonal antibodies 132 and 8D5 in immuno-cytochemistry2015

    • Author(s)
      Tanaka, M., Masujin, K., Yamasaki, T., Suzuki, A., Hasebe, R., and Horiuchi, M
    • Organizer
      The 63rd Annual Meeting of The Japanese Sciety for Virology
    • Place of Presentation
      Fukuoka, Fukuoka, Japan
    • Year and Date
      2015-11-22
    • Related Report
      2015 Research-status Report
  • [Presentation] Comparison of PrPSc-specific staining with two anti-PrP monoclonal antibodies in immuno-cytochemistry2015

    • Author(s)
      Tanaka, M., Masujin, K., Yamasaki, T., Suzuki, A., Hasebe, R., and Horiuchi, M
    • Organizer
      APPS2015
    • Place of Presentation
      Kanazawa, Ishikawa, Japan
    • Year and Date
      2015-09-04
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Increased membrane permeability by complement factors affect PrPSc deposition in neurons2015

    • Author(s)
      Hasebe, R., Suzuki, A., Yamasaki, T., and Horiuchi, M.
    • Organizer
      Prion2015
    • Place of Presentation
      Fort Collins, Colorado, USA
    • Year and Date
      2015-05-26
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Analysis of activation state of astrocytes with the progression of prion diseases2015

    • Author(s)
      Kuroda, M., Yamasaki, T.,Suzuki, A., Hasebe, R., and Horiuchi, M
    • Organizer
      Prion2015
    • Place of Presentation
      Fort Collins, Colorado, USA
    • Year and Date
      2015-05-26
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Highthrouput detection of PrPSc from prion-infected cells without PK treatment: Cell-based ELISA for novel screening method for anti-prion compounds2015

    • Author(s)
      Shan, Z., Yamasaki, T., Suzuki, A., Hasebe, R., and Horiuchi, M
    • Organizer
      Prion2015
    • Place of Presentation
      Fort Collins, Colorado, USA
    • Year and Date
      2015-05-26
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Flow cytometric detection of PrPSc in neurons from prion-infected mouse brain2015

    • Author(s)
      Yamasaki, T., Suzuki, A., Hasebe, R., and Horiuchi, M
    • Organizer
      Prion2015
    • Place of Presentation
      Fort Collins, Colorado, USA
    • Year and Date
      2015-05-26
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] プリオンの増殖に関わる細胞内小器官の同定ープリオン感染マウス脳組織の免疫組織学的解析からー2014

    • Author(s)
      堀内 基広、斉藤 真央、長谷部 理絵、山崎 剛士
    • Organizer
      第62回日本ウイルス学会学術集会
    • Place of Presentation
      パシフィコ横浜、神奈川県
    • Year and Date
      2014-11-10 – 2014-11-12
    • Related Report
      2014 Research-status Report
  • [Presentation] フローサイトメトリーによるプリオン感染細胞の検出2014

    • Author(s)
      山崎 剛士、鈴木 章夫、長谷部 理絵、堀内 基広
    • Organizer
      第62回日本ウイルス学会学術集会
    • Place of Presentation
      パシフィコ横浜、神奈川県
    • Year and Date
      2014-11-10 – 2014-11-12
    • Related Report
      2014 Research-status Report
  • [Presentation] 補対反応はプリオン感染マウス大脳皮質由来初代培養神経細胞の細胞膜透過性を亢進し、異常型プリオンタンパク質の蓄積量を変化させる2014

    • Author(s)
      長谷部 理絵、鈴木 章夫、山崎 剛士、堀内 基広
    • Organizer
      第62回日本ウイルス学会学術集会
    • Place of Presentation
      パシフィコ横浜、神奈川県
    • Year and Date
      2014-11-10 – 2014-11-12
    • Related Report
      2014 Research-status Report
  • [Presentation] プリオンの病態進行に伴うアストロサイト活性化状態の解析2014

    • Author(s)
      黒田 弥乃梨、山崎 剛士、鈴木 章夫、長谷部 理絵、堀内 基広
    • Organizer
      第62回日本ウイルス学会学術集会
    • Place of Presentation
      パシフィコ横浜、神奈川県
    • Year and Date
      2014-11-10 – 2014-11-12
    • Related Report
      2014 Research-status Report
  • [Presentation] プリオン感染マウス脳由来アストロサイトの活性化状態の解析2014

    • Author(s)
      黒田 弥乃梨、山崎 剛士、鈴木 章夫、長谷部 理絵、堀内 基広
    • Organizer
      第157回日本獣医学会学術集会
    • Place of Presentation
      北海道大学、北海道
    • Year and Date
      2014-09-09 – 2014-09-12
    • Related Report
      2014 Research-status Report
  • [Presentation] 大脳皮質由来初代培養神経細胞におけるプリオン感染の解析2014

    • Author(s)
      藤原 愛、山崎 剛士、鈴木 章夫、長谷部 理絵、堀内 基広
    • Organizer
      第157回日本獣医学会学術集会
    • Place of Presentation
      北海道大学、北海道
    • Year and Date
      2014-09-09 – 2014-09-12
    • Related Report
      2014 Research-status Report
  • [Presentation] Comparison of anti-prion mechanism of four different anti-prion compounds, anti-monoclonal antibody 44B1, pentosan polysulfate, chlorpromazine and U18666A in prion-infected mouse neuroblastoma cells2014

    • Author(s)
      Yamasaki T, Suzuki A, Hasebe R, Horiuchi M
    • Organizer
      Asian Pacific Prion Symposium
    • Place of Presentation
      Jeju International Convention Center, Kore
    • Year and Date
      2014-07-06 – 2014-07-07
    • Related Report
      2014 Research-status Report
  • [Presentation] Analysis of prion infection in primary cortical neurons2014

    • Author(s)
      Fujiwara A, Sassa Y, Yamasaki T, Hasebe R, Horiuchi M
    • Organizer
      Asian Pacific Prion Symposium
    • Place of Presentation
      Jeju International Convention Center, Korea
    • Year and Date
      2014-07-06 – 2014-07-07
    • Related Report
      2014 Research-status Report
  • [Presentation] High throughput detection of PrPSc from prion-infected cells without PK-treatment: cell based ELISA for novel screening for anti-prion compounds2014

    • Author(s)
      Shan Z, Yamasaki T, Suzuki A, Hasebe R, Horiuchi M
    • Organizer
      Asian Pacific Prion Symposium
    • Place of Presentation
      Jeju International Convention Center, Korea
    • Year and Date
      2014-07-06 – 2014-07-07
    • Related Report
      2014 Research-status Report
  • [Presentation] Reaction kinetic analysis of anti-PrP monoclonal antibody mAb 132 by surface plasmon resonance2014

    • Author(s)
      Suzuki A, Yamasaki T, Hasebe R, Horiuchi M
    • Organizer
      Asian Pacific Prion Symposium
    • Place of Presentation
      Jeju International Convention Center, Korea
    • Year and Date
      2014-07-06 – 2014-07-07
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2018-03-22  

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