| Project/Area Number |
26450401
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Veterinary medical science
|
|---|
| Research Institution | Gifu University |
|---|
Principal Investigator |
Nagano Isao 岐阜大学, 大学院医学系研究科, 准教授 (40283296)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
前川 洋一 岐阜大学, 大学院医学系研究科, 教授 (10294670)
鎌足 雄司 岐阜大学, 生命科学総合研究支援センター, 助教 (70342772)
呉 志良 岐阜大学, 大学院医学系研究科, 助教 (90313874)
|
|---|
| Project Period (FY) |
2014-04-01 – 2017-03-31
|
| Project Status |
Completed (Fiscal Year 2016)
|
| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
|---|
| Keywords | 旋毛虫 / 即時型アレルギー / 免疫抑制 / 分泌タンパク質 / サイトカイン / 組換えタンパク質 / スギ花粉 / 高次構造解析 |
|---|
| Outline of Final Research Achievements |
It is proposed that Trichinella infection or Trichinella-derived molecules（Tp53）modulate host’s immunity.
In this study, we investigated the effect of Trichinella infection or Tp53 on immediate hypersensitivity in mice sensitized with Japanese cedar pollen. As a result, trichinella infection or Tp53 administration attenuated inflammatory reactions in the lungs, and production of specific IgE, IgG2a, IgG2b antibody decreased. In addition, production of IL-4 and IL-17 in infected mouse spleen cells or splenocytes treated with Tp53 was suppressed. And our observation showed that the Tp53 suppressed NF-κB signal pathway of macrophage, as indicated by the results of inhibition of LPS stimulation-induced NF-κB p65 nuclear translocation, phosphrylation of NF-κB signal proteins (IKK, p65 and IκBα) and degradation of IκBα. The results suggest that the Tp53 protein may modulate host immune response by inhibiting macrophage activation via specifically affecting NF-κB signal pathway.
|
