Bacteriological and epidemiological studies for elucidating the pathophysiology of bovine digital dermatitis and its control

• Project/Area Number: 26450406
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Veterinary medical science
• Research Institution: University of Miyazaki
• Principal Investigator: Yamazaki Wataru 宮崎大学, 農学部, 准教授 (70393262)
• Co-Investigator(Renkei-kenkyūsha): MISAWA Naoaki 宮崎大学, 産業動物防疫リサーチセンター, 教授 (20229678)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 牛 / 趾乳頭腫症 / 生菌製剤 / 抗生物質 / Treponema属菌 / 細菌叢解析 / リアルタイムPCR / LAMP / Allyl isothiocyanate / 削蹄 / 牛趾乳頭腫症 / T.medium/vincentii / T. denticola/putidum / T. phagedenis / 次世代シークエンサー / 蹄 / 細菌叢 / T. medium/vincentii / クローニング / Treponema vincentii

Outline of Final Research Achievements

The possibility of preventing the recurrence of bovine digintal dermatitis (PDD) and re-infection of Treponema species was demonstrated by using the combined treatment of application of allyl isothiocyanate (AITC) and hoofing. Microbiome analysis indiated that T. medium-like and T. denticola-like isolates showed the possibility of playing an important role in PDD deformity. We developed three real-time PCR and four LAMP assays that can specifically detect and distinguish three different Treponema species at both genus and species levels.

Research Products

• [Int'l Joint Research] Wisconsin大学獣医学部(米国)
• [Int'l Joint Research] Wisconsin大学/獣医学部/Dorte Dopfer研究室(アメリカ合衆国)
• [Int'l Joint Research] ウィスコンシン大学獣医学部(アメリカ合衆国)
• [Journal Article] Clinical and microbiological characteristics of patients with bacteremia caused by Campylobacter species with an emphasis on the subspecies of C. fetus (2019)
  • Author(s): Liu, Y. H., Yamazaki, W., Huang, Y. T., Liao, C. H., Sheng, W. H., Hsueh, P. R.
  • Journal Title: J. Microbiol. Immunol. Infect. Volume: 印刷中 Pages: 1-10
  • DOI: 10.1016/j.jmii.2017.07.009
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Combination effect of allyl isothiocyanate and hoof trimming on bovine digital dermatitis (2018)
  • Author(s): Yamamoto, T., Manabe, H., Yamazaki, W., Misawa, N., Takahashi, M., Okada, K.
  • Journal Title: J. Vet. Med. Sci. Volume: 7 Issue: 7 Pages: 1080-1085
  • DOI: 10.1292/jvms.18-0097
  • NAID: 130006733252
  • Peer Reviewed / Open Access
• [Journal Article] Development of real-time PCR and loop-mediated isothermal amplification (LAMP) assays for the differential detection of digital dermatitis associated treponemes (2017)
  • Author(s): Anklam, K., Kulow, M., Yamazaki, W., Dopfer, D.
  • Journal Title: PLOS One Volume: 12 Pages: 1-13
  • DOI: 10.1371/journal.pone.0178349
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Direct detection and characterisation of foot-and-mouth disease virus in east Africa using a field-ready real-time PCR platform (2017)
  • Author(s): Howson, E.L.A., Armson, B.N., Lyons, A., Chepkwony, E., Kasanga, C.J., Kandusi, S., Ndusilo, N., Yamazaki, W., Gizaw, D., Cleaveland, S., Lembo, T., Rauh, R., Nelson, W.M., Wood, B.A., Mioulet, V., King, D.P., Fowler, V. L.
  • Journal Title: Transboundary Emerging Diseases Volume: 12 Pages: 1-11
  • DOI: 10.1111/tbed.12684
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Distinct Campylobacter fetus lineages adapted as livestock pathogens and intestinal pathoboints in the human microbiota (2017)
  • Author(s): Iraola, G., Forster, S.C., Kumar, N., Lehours, P., García-Peña, F.J., Paolicchi, F., Morsella, C., Hotzel, H., Hung, L.Y., Hsueh, P.R., Vidal, A., Lévesque, S., Bekal, S., Yamazaki,W., 他14名
  • Journal Title: Nature Communications Volume: 8 Pages: 1-8
  • DOI: 10.1038/s41467-017-01449-9
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] High Prevalence of <i>Campylobacter</i> in Broiler Flocks is a Crucial Factor for Frequency of Food Poisoning in Humans (2017)
  • Author(s): Yamazaki, W., Sabike, I. I., Sekiguchi, S.
  • Journal Title: Japanese Journal of Infectious Diseases Volume: 70 Issue: 6 Pages: 691-692
  • DOI: 10.7883/yoken.JJID.2017.132
  • NAID: 130006210307
  • Peer Reviewed / Open Access
• [Journal Article] Development of LAMP assays for the molecular detection of taeniid infection in canine in Tibetan rural area (2017)
  • Author(s): Feng, K., Li, W., Guo, Z., Duo, H., Fu, Y., Shen, X., Tie, C., E, R., Xiao, C., Luo, Y., Qi, G., Ni, M., Ma, Q., Yamazaki, W., Yoshida, A., Horii, Y., Yagi, K., Nonaka, N.
  • Journal Title: J. Vet. Med. Sci. Volume: 79 Issue: 12 Pages: 1986-1993
  • DOI: 10.1292/jvms.17-0430
  • NAID: 130006267659
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Assessment of the Campylobacter jejuni and C. coli in broiler chicken ceca by conventional culture and loop-mediated isothermal amplification method (2017)
  • Author(s): Sabike, I. I., Uemura, R., Kirino, Y., Mekata, H., Sekiguchi, S., Farid, A. S., Goto, Y., Horii, Y., Yamazaki, W.
  • Journal Title: Food Control Volume: 74 Pages: 107-111
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Effect of antibiotic pre-treatment and pathogen challenge on the intestinal microbiota in mice (2016)
  • Author(s): Iizumi, T., Taniguchi, T., Yamazaki, W., Vilmen, G., Alekseyenko, A., Gao, Z., Perez-Perez, G., Blaser, M. J.
  • Journal Title: Gut Pathogens Volume: 8 Pages: 1-10
  • DOI: 10.1186/s13099-016-0143-z
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Use of direct LAMP screening of broiler fecal samples for Campylobacter jejuni and Campylobacter coli in the positive flock identification strategy (2016)
  • Author(s): Sabike, I. I., Uemura, R., Kirino, Y., Mekata, H., Sekiguchi, S., Okabayashi, T., Goto, Y., Yamazaki, W.
  • Journal Title: Frontiers in Microbiology Volume: 7 Pages: 1-5
  • DOI: 10.3389/fmicb.2016.01582
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] An improved loop-mediated isothermal amplification assay for the detection of <i>Mycoplasma bovis</i> (2016)
  • Author(s): Higa, Y., Uemura, R., Yamazaki, W., Goto, Y., Sueyoshi, M.
  • Journal Title: J. Vet. Med. Sci. Volume: 78 Issue: 8 Pages: 1343-1346
  • DOI: 10.1292/jvms.15-0459
  • NAID: 130005261539
  • Peer Reviewed / Open Access
• [Journal Article] Campylobacter and Salmonella are prevalent in broiler farms in Kyushu, Japan: Results of a 2-year distribution (2016)
  • Author(s): Yamazaki, W., Uemura, R., Sekiguchi, S., Dong, J.B., Watanabe, S., Kirino, Y., Mekata, H., Nonaka, N., Norimine, J., Sueyoshi, M., Goto, Y., Horii, Y., Kurogi, M., Yoshino, S., Misawa, N.
  • Journal Title: Journal of Applied Microbiology Volume: 120 Pages: 1711-1722
  • DOI: 10.1111/jam.13141
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] The pathogenic potential of <i>Helicobacter cinaedi</i> isolated from non-human sources: adherence, invasion and translocation ability in polarized intestinal epithelial Caco-2 cells <i>in vitro</i> (2016)
  • Author(s): Taniguchi, T., Yamazaki, W., Saeki, Y., Takajo, I., Okayama, A., Hayashi, T., Misawa, N.
  • Journal Title: J. Vet. Med. Sci. Volume: 78 Issue: 4 Pages: 627-632
  • DOI: 10.1292/jvms.15-0595
  • NAID: 130007331500
  • Peer Reviewed / Open Access
• [Journal Article] Loop-Mediated Isothermal Amplification (LAMP) for Detection of <i>Campylobacter jejuni</i> and <i>C. coli</i> in Thai Children with Diarrhea (2015)
  • Author(s): Pham NTK, Trinh QD, Khamrin P, Ukarapol N, Kongsricharoern T, Yamazaki W, Okitsu S, Maneekarn N, Hayakawa S, Ushijima H
  • Journal Title: Japanese Journal of Infectious Diseases Volume: 印刷中 Issue: 5 Pages: 432-433
  • DOI: 10.7883/yoken.JJID.2014.450
  • NAID: 130005098918
  • Peer Reviewed / Open Access
• [Journal Article] Improvement of the quantitation method for the tdh (+) Vibrio parahaemolyticus in molluscan shellfish based on most-probable-number, immunomagnetic separation, and loop-mediated isothermal amplification (2015)
  • Author(s): Escalante-Maldonado, O. R., Kayali,A. Y., Yamazaki, W.,Vuddhakul, Nakaguchi, Y., Nishibuchi, M.
  • Journal Title: Fronters in Microbiology Volume: 6 Pages: 1-10
  • DOI: 10.3389/fmicb.2015.00270
  • NAID: 120005690133
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] Highly sensitive detection system for viral RNAs using a combination of virion concentration and simple elusion techniques (2018)
  • Author(s): Yamazaki, W.
  • Organizer: 2nd Scientific meeting on FMD between regional reference laboratory for FMD in Southeast Asia and Exotic disease research station, NIAH, NARO
  • Int'l Joint Research / Invited
• [Presentation] Campylobacter prevalence in broiler flocks highly correlates with food poisoning frequency in Japan: assessment in broiler cecal and fecal samples by conventional culture and loop-mediated isothermal amplification methods (2017)
  • Author(s): Sabike, I. I., Uemura, R., Kirino, Y., Mekata, H., Sekiguchi, S., Okabayashi, T., Farid, A. S., Goto, Y., Horii, Y., Yamazaki, W.
  • Organizer: 51th Conference Cholera and other bacterial enteric infections US-Japan cooperative medical science program
  • Place of Presentation: Seoul, Republic of Korea
  • Year and Date: 2017-02-08
  • Int'l Joint Research / Invited
• [Presentation] Highly sensitive detection of avian influenza virus in environmental samples using a new virus concentration technique (2017)
  • Author(s): Yamazaki, W.
  • Organizer: JSPS core-to-core 1st meeting/7th CADIC (Center for Animal Diseases Control) International symposium
  • Int'l Joint Research / Invited
• [Presentation] 携帯リアルタイムLAMP機器を用いた口蹄疫の野外即時診断法の開発と評価 (2017)
  • Author(s): 山崎 渉, Howson E., Armson B., Mioulet V., King D., 三澤尚明, Fowler V., Kasanga C.
  • Organizer: 第160回日本獣医学会総会
• [Presentation] Development of LAMP assays for the molecular detection of taeniid cestodes of canine in Tibetan rural area (2017)
  • Author(s): 山崎 渉
  • Organizer: 第160回日本獣医学会総会
• [Presentation] LAMP法を用いたMycobacterium avium 3亜種の迅速かつ正確な検出法の開発 (2017)
  • Author(s): 屋敷奈津, 山崎 渉, 後藤義孝
  • Organizer: 平成29年度日本獣医公衆衛生学会（九州地区）
• [Presentation] LAMP法による口蹄疫と類似疾病の簡易迅速診断 (2017)
  • Author(s): 山崎 渉
  • Organizer: 第2回宮崎大学産業動物防疫セミナー（主催：宮崎大学産業動物防疫リサーチセンター）
• [Presentation] Development of LAMP assay for rapid and sensitive diagnosis of African swine fever in field settings (2017)
  • Author(s): Yamazaki, W.
  • Organizer: Korea-Thailand-Japan Joint Seminar, 2017 -How should we tackle with animal diseases for the significant growth of livestock industry in Asia?
  • Place of Presentation: 宮崎大学（宮崎市、宮崎県）
  • Invited
• [Presentation] LAMPを用いた鶏盲腸内容物内カンピロバクターのスクリーニングと肉用鶏保菌率が食中毒発生に及ぼす影響 (2016)
  • Author(s): Sabike, I.I., 上村涼子, 桐野有美, 目堅博久, 関口 敏, Farid, A.S., 後藤義孝, 堀井洋一郎, 山崎 渉
  • Organizer: 第112回 日本食品衛生学会学術講演会
  • Place of Presentation: 函館国際観光ホテル（函館市、北海道）
  • Year and Date: 2016-10-27
• [Presentation] カンピロバクター食中毒の発生頻度は養鶏場ブロイラーの保菌率と相関する (2016)
  • Author(s): Sabike, I.I., 上村涼子, 桐野有美, 目堅博久, 関口 敏, Farid, A.S., 後藤義孝, 堀井洋一郎, 山崎 渉
  • Organizer: 第50回腸炎ビブリオシンポジウム
  • Place of Presentation: 大阪大学（吹田市、大阪）
  • Year and Date: 2016-10-20
• [Presentation] Novel molecular diagnositic technique for rapid and sensitive detection of foot-and-mouth disease virus (FMDV) (2016)
  • Author(s): Yamazaki, W.
  • Organizer: 3rd International conference on biotechnology application in agriculture
  • Place of Presentation: Sharm El-Sheikh, Egypt
  • Year and Date: 2016-04-05
  • Int'l Joint Research / Invited
• [Presentation] Analysis of chicken cecal contents by pyrosequencing to search probiotics for Campylobacter (2016)
  • Author(s): Yamazaki, W., Uemura,R., ekiguchi, S., Taniguchi, T., Misawa, N.
  • Organizer: 50th Conference Cholera and other bacterial enteric infections US-Japan cooperative medical science program
  • Place of Presentation: Washington D.C., USA
  • Year and Date: 2016-01-11
  • Int'l Joint Research / Invited
• [Presentation] 口蹄疫・アフリカ豚コレラの発生状況と高感度な簡易診断技術開発のフィージビリティスタディ (2016)
  • Author(s): 山崎 渉
  • Organizer: 宮崎大学テニュアトラック推進機構主催セミナー
  • Place of Presentation: 宮日会館（宮崎市、宮崎県）
• [Presentation] 鶏盲腸におけるカンピロバクターへのプロバイオティクス探索を目的としたパイロシークエンス解析 (2015)
  • Author(s): 山崎 渉 上村涼子 関口敏 谷口喬子 三澤尚明
  • Organizer: 第36回日本食品微生物学会総会
  • Place of Presentation: 川崎市教育文化会館（神奈川県川崎市）
  • Year and Date: 2015-11-12
• [Presentation] 鶏盲腸におけるカンピロバクターへのプロバイオティクス探索を目的としたパイロシークエンス解析 (2015)
  • Author(s): 山崎 渉 上村涼子 関口敏 谷口喬子 三澤尚明
  • Organizer: 平成27年度日本獣医公衆衛生学会(九州地区)
  • Place of Presentation: メルパルク熊本(熊本県熊本市）
  • Year and Date: 2015-10-16
• [Presentation] 牛趾乳頭腫症病変部の治療前後における細菌叢の包括的比較解析 (2015)
  • Author(s): 山崎 渉 後藤恭宏 岡田啓司 谷口喬子 林 哲也 三澤尚明
  • Organizer: 第158回日本獣医学会総会
  • Place of Presentation: 北里大学（青森県十和田市）
  • Year and Date: 2015-09-07
• [Presentation] Introduction of loop-mediated isothermal amplification (LAMP) assay for rapid and sensitive detection of microbial pathogens (2015)
  • Author(s): Yamazaki W
  • Organizer: Molecular diagnosis in viral vesicular diseases
  • Place of Presentation: Sao Paulo, Brazil
  • Year and Date: 2015-07-28
  • Int'l Joint Research / Invited
• [Presentation] 牛趾乳頭腫症病変部の治療前後における細菌叢の比較解析. (2014)
  • Author(s): 山崎 渉 谷口喬子 後藤恭宏 林 哲也 三澤尚明.
  • Organizer: 第 67 回日本細菌学会 九州支部総会.
  • Place of Presentation: 鹿児島市
  • Year and Date: 2014-09-05 – 2014-09-06
• [Book] Ecology, virulence and detection of pathogenic and pandemic Vibrio parahaemolyticus (2016)
  • Author(s): Yamazaki, W. et.al.
  • Total Pages: 134
  • Publisher: Frontiers Media
• [Book] 大・中・小動物実験プロトコール (2016)
  • Author(s): 山崎 渉他
  • Total Pages: 146
  • Publisher: 宮日文化情報センター
• [Book] 大・中・小動物実験プロトコール (2016)
  • Author(s): 宮崎大学動物実験プロトコール編集委員会 （山崎 渉他32名）
  • Total Pages: 146
  • Publisher: 宮日文化情報センター
• [Remarks] 宮崎大学 農学部 獣医学科
  • URL: http://www.agr.miyazaki-u.ac.jp/~vet/vet_HP1.htm
• [Remarks] 宮崎大学農学部獣医学科
  • URL: http://www.agr.miyazaki-u.ac.jp/~vet/vet_HP1.htm
• [Remarks] 宮崎大学産業動物防疫リサーチセンター
  • URL: http://www.miyazaki-u.ac.jp/cadic/query/query_index.php
• [Patent(Industrial Property Rights)] 複数のプライマーセットを組み合わせたLAMP法を用いたアフリカ豚コレラウイルスの迅速検出法 (2016)
  • Inventor(s): 山崎 渉
  • Industrial Property Rights Holder: 国立大学法人宮崎大学
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2016-112827
  • Filing Date: 2016-06-06
  • Application Year: 2016