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Bacteriological and epidemiological studies for elucidating the pathophysiology of bovine digital dermatitis and its control

Research Project

Project/Area Number 26450406
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Veterinary medical science
Research InstitutionUniversity of Miyazaki

Principal Investigator

Yamazaki Wataru  宮崎大学, 農学部, 准教授 (70393262)

Co-Investigator(Renkei-kenkyūsha) MISAWA Naoaki  宮崎大学, 産業動物防疫リサーチセンター, 教授 (20229678)
Project Period (FY) 2014-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords牛 / 趾乳頭腫症 / 生菌製剤 / 抗生物質 / Treponema属菌 / 細菌叢解析 / リアルタイムPCR / LAMP / Allyl isothiocyanate / 削蹄 / 牛趾乳頭腫症 / T.medium/vincentii / T. denticola/putidum / T. phagedenis / 次世代シークエンサー / 蹄 / 細菌叢 / T. medium/vincentii / クローニング / Treponema vincentii
Outline of Final Research Achievements

The possibility of preventing the recurrence of bovine digintal dermatitis (PDD) and re-infection of Treponema species was demonstrated by using the combined treatment of application of allyl isothiocyanate (AITC) and hoofing. Microbiome analysis indiated that T. medium-like and T. denticola-like isolates showed the possibility of playing an important role in PDD deformity. We developed three real-time PCR and four LAMP assays that can specifically detect and distinguish three different Treponema species at both genus and species levels.

Report

(5 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (43 results)

All 2018 2017 2016 2015 2014 Other

All Int'l Joint Research (3 results) Journal Article (15 results) (of which Int'l Joint Research: 9 results,  Peer Reviewed: 15 results,  Open Access: 14 results,  Acknowledgement Compliant: 1 results) Presentation (18 results) (of which Int'l Joint Research: 6 results,  Invited: 7 results) Book (3 results) Remarks (3 results) Patent(Industrial Property Rights) (1 results)

  • [Int'l Joint Research] Wisconsin大学獣医学部(米国)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] Wisconsin大学/獣医学部/Dorte Dopfer研究室(米国)

    • Related Report
      2016 Research-status Report
  • [Int'l Joint Research] ウィスコンシン大学獣医学部(米国)

    • Related Report
      2015 Research-status Report
  • [Journal Article] Clinical and microbiological characteristics of patients with bacteremia caused by Campylobacter species with an emphasis on the subspecies of C. fetus2018

    • Author(s)
      Liu, Y. H., Yamazaki, W., Huang, Y. T., Liao, C. H., Sheng, W. H., Hsueh, P. R.
    • Journal Title

      Journal of Microbiology, Immunology and Infection

      Volume: 印刷中 Issue: 1 Pages: 1-10

    • DOI

      10.1016/j.jmii.2017.07.009

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Combination effect of allyl isothiocyanate and hoof trimming on bovine digital dermatitis2018

    • Author(s)
      Yamamoto, T., Manabe, H., Yamazaki, W., Misawa, N., Takahashi, M., Okada, K.
    • Journal Title

      Journal of Veterinary Medical Science

      Volume: 80 Issue: 7 Pages: 1080-1085

    • DOI

      10.1292/jvms.18-0097

    • NAID

      130007404170

    • ISSN
      0916-7250, 1347-7439
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Development of real-time PCR and loop-mediated isothermal amplification (LAMP) assays for the differential detection of digital dermatitis associated treponemes2017

    • Author(s)
      Anklam, K., Kulow, M., Yamazaki, W., Dopfer, D.
    • Journal Title

      PLOS One

      Volume: 12 Issue: 5 Pages: 1-13

    • DOI

      10.1371/journal.pone.0178349

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Direct detection and characterisation of foot-and-mouth disease virus in east Africa using a field-ready real-time PCR platform2017

    • Author(s)
      Howson, E.L.A., Armson, B.N., Lyons, A., Chepkwony, E., Kasanga, C.J., Kandusi, S., Ndusilo, N., Yamazaki, W., Gizaw, D., Cleaveland, S., Lembo, T., Rauh, R., Nelson, W.M., Wood, B.A., Mioulet, V., King, D.P., Fowler, V. L.
    • Journal Title

      Transboundary Emerging Diseases

      Volume: 12 Issue: 1 Pages: 1-11

    • DOI

      10.1111/tbed.12684

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Distinct Campylobacter fetus lineages adapted as livestock pathogens and intestinal pathoboints in the human microbiota2017

    • Author(s)
      Iraola, G., Forster, S.C., Kumar, N., Lehours, P., García-Peña, F.J., Paolicchi, F., Morsella, C., Hotzel, H., Hung, L.Y., Hsueh, P.R., Vidal, A., Lévesque, S., Bekal, S., Yamazaki,W., 他14名
    • Journal Title

      Nature Communications

      Volume: 8 Issue: 1 Pages: 1-8

    • DOI

      10.1038/s41467-017-01449-9

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] High Prevalence of <i>Campylobacter</i> in Broiler Flocks is a Crucial Factor for Frequency of Food Poisoning in Humans2017

    • Author(s)
      Yamazaki, W., Sabike, I. I., Sekiguchi, S.
    • Journal Title

      Japanese Journal of Infectious Diseases

      Volume: 70 Issue: 6 Pages: 691-692

    • DOI

      10.7883/yoken.JJID.2017.132

    • NAID

      130006210307

    • ISSN
      1344-6304, 1884-2836
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Development of LAMP assays for the molecular detection of taeniid infection in canine in Tibetan rural area2017

    • Author(s)
      Feng, K., Li, W., Guo, Z., Duo, H., Fu, Y., Shen, X., Tie, C., E, R., Xiao, C., Luo, Y., Qi, G., Ni, M., Ma, Q., Yamazaki, W., Yoshida, A., Horii, Y., Yagi, K., Nonaka, N.
    • Journal Title

      Journal of Veterinary Medical Science

      Volume: 79 Issue: 12 Pages: 1986-1993

    • DOI

      10.1292/jvms.17-0430

    • NAID

      130006267659

    • ISSN
      0916-7250, 1347-7439
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Assessment of the Campylobacter jejuni and C. coli in broiler chicken ceca by conventional culture and loop-mediated isothermal amplification method2017

    • Author(s)
      Sabike, I. I., Uemura, R., Kirino, Y., Mekata, H., Sekiguchi, S., Farid, A. S., Goto, Y., Horii, Y., Yamazaki, W.
    • Journal Title

      Food Control

      Volume: 74 Pages: 107-111

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Effect of antibiotic pre-treatment and pathogen challenge on the intestinal microbiota in mice2016

    • Author(s)
      Iizumi, T., Taniguchi, T., Yamazaki, W., Vilmen, G., Alekseyenko, A., Gao, Z., Perez-Perez, G., Blaser, M. J.
    • Journal Title

      Gut Pathogens

      Volume: 8 Issue: 1 Pages: 1-10

    • DOI

      10.1186/s13099-016-0143-z

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Use of direct LAMP screening of broiler fecal samples for Campylobacter jejuni and Campylobacter coli in the positive flock identification strategy2016

    • Author(s)
      Sabike, I. I., Uemura, R., Kirino, Y., Mekata, H., Sekiguchi, S., Okabayashi, T., Goto, Y., Yamazaki, W.
    • Journal Title

      Frontiers in Microbiology

      Volume: 7 Pages: 1-5

    • DOI

      10.3389/fmicb.2016.01582

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] An improved loop-mediated isothermal amplification assay for the detection of <i>Mycoplasma bovis</i>2016

    • Author(s)
      Higa, Y., Uemura, R., Yamazaki, W., Goto, Y., Sueyoshi, M.
    • Journal Title

      Journal of Veterinary Medical Science

      Volume: 78 Issue: 8 Pages: 1343-1346

    • DOI

      10.1292/jvms.15-0459

    • NAID

      130005261539

    • ISSN
      0916-7250, 1347-7439
    • Related Report
      2016 Research-status Report 2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Campylobacter and Salmonella are prevalent in broiler farms in Kyushu, Japan: Results of a 2-year distribution and circulation dynamics audit2016

    • Author(s)
      Yamazaki, W., Uemura, R., Sekiguchi, S., Dong, J-B.,Watanabe, S., Kirino, Y., Mekata, H., Nonaka, N., Norimine, Ju., Sueyoshi, M., Goto, Y., Horii, Y., Kurogi, M., Yoshino, S., Misawa, N.
    • Journal Title

      Journal of Applied Microbiology

      Volume: 120 Issue: 6 Pages: 1711-1722

    • DOI

      10.1111/jam.13141

    • Related Report
      2016 Research-status Report 2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] The pathogenic potential of <i>Helicobacter cinaedi</i> isolated from non-human sources: adherence, invasion and translocation ability in polarized intestinal epithelial Caco-2 cells <i>in vitro</i>2016

    • Author(s)
      Taniguchi, T., Yamazaki, W., Saeki, Y., Takajo, I., Okayama, A., Hayashi, T., Misawa, N.
    • Journal Title

      Journal of Veterinary Medical Science

      Volume: 78 Issue: 4 Pages: 627-632

    • DOI

      10.1292/jvms.15-0595

    • NAID

      130007331500

    • ISSN
      0916-7250, 1347-7439
    • Related Report
      2016 Research-status Report 2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Loop-Mediated Isothermal Amplification (LAMP) for Detection of <i>Campylobacter jejuni</i> and <i>C. coli</i> in Thai Children with Diarrhea2015

    • Author(s)
      Pham NTK, Trinh QD, Khamrin P, Ukarapol N, Kongsricharoern T, Yamazaki W, Okitsu S, Maneekarn N, Hayakawa S, Ushijima H
    • Journal Title

      Japanese Journal of Infectious Diseases

      Volume: 68 Issue: 5 Pages: 432-433

    • DOI

      10.7883/yoken.JJID.2014.450

    • NAID

      130005098918

    • ISSN
      1344-6304, 1884-2836
    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Improvement of the quantitation method for the tdh+ Vibrio parahaemolyticus in molluscan shellfish based on most-probablenumber,immunomagnetic separation, and loop-mediated isothermal amplification2015

    • Author(s)
      Escalante-Maldonado, O. R., Kayali,A. Y., Yamazaki, W.,Vuddhakul, Nakaguchi, Y., Nishibuchi, M.
    • Journal Title

      Frontiers in microbiology

      Volume: 6 Pages: 1-10

    • DOI

      10.3389/fmicb.2015.00270

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] Highly sensitive detection system for viral RNAs using a combination of virion concentration and simple elusion techniques2018

    • Author(s)
      Yamazaki, W.
    • Organizer
      2nd Scientific meeting on FMD between regional reference laboratory for FMD in Southeast Asia and Exotic disease research station, NIAH, NARO
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Campylobacter prevalence in broiler flocks highly correlates with food poisoning frequency in Japan: assessment in broiler cecal and fecal samples by conventional culture and loop-mediated isothermal amplification methods2017

    • Author(s)
      Sabike, I. I., Uemura, R., Kirino, Y., Mekata, H., Sekiguchi, S., Okabayashi, T., Farid, A. S., Goto, Y., Horii, Y., Yamazaki, W.
    • Organizer
      51th Conference Cholera and other bacterial enteric infections US-Japan cooperative medical science program
    • Place of Presentation
      Seoul, Republic of Korea
    • Year and Date
      2017-02-08
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Highly sensitive detection of avian influenza virus in environmental samples using a new virus concentration technique2017

    • Author(s)
      Yamazaki, W.
    • Organizer
      JSPS core-to-core 1st meeting/7th CADIC (Center for Animal Diseases Control) International symposium
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 携帯リアルタイムLAMP機器を用いた口蹄疫の野外即時診断法の開発と評価2017

    • Author(s)
      山崎 渉, Howson E., Armson B., Mioulet V., King D., 三澤尚明, Fowler V., Kasanga C.
    • Organizer
      第160回日本獣医学会総会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Development of LAMP assays for the molecular detection of taeniid cestodes of canine in Tibetan rural area2017

    • Author(s)
      山崎 渉
    • Organizer
      第160回日本獣医学会総会
    • Related Report
      2017 Annual Research Report
  • [Presentation] LAMP法を用いたMycobacterium avium 3亜種の迅速かつ正確な検出法の開発2017

    • Author(s)
      屋敷奈津, 山崎 渉, 後藤義孝
    • Organizer
      平成29年度日本獣医公衆衛生学会(九州地区)
    • Related Report
      2017 Annual Research Report
  • [Presentation] LAMP法による口蹄疫と類似疾病の簡易迅速診断2017

    • Author(s)
      山崎 渉
    • Organizer
      第2回宮崎大学産業動物防疫セミナー(主催:宮崎大学産業動物防疫リサーチセンター)
    • Related Report
      2017 Annual Research Report
  • [Presentation] Development of LAMP assay for rapid and sensitive diagnosis of African swine fever in field settings2017

    • Author(s)
      Yamazaki, W.
    • Organizer
      Korea-Thailand-Japan Joint Seminar, 2017 -How should we tackle with animal diseases for the significant growth of livestock industry in Asia?
    • Place of Presentation
      宮崎大学(宮崎市、宮崎県)
    • Related Report
      2016 Research-status Report
    • Invited
  • [Presentation] LAMPを用いた鶏盲腸内容物内カンピロバクターのスクリーニングと肉用鶏保菌率が食中毒発生に及ぼす影響2016

    • Author(s)
      Sabike, I.I., 上村涼子, 桐野有美, 目堅博久, 関口 敏, Farid, A.S., 後藤義孝, 堀井洋一郎, 山崎 渉
    • Organizer
      第112回 日本食品衛生学会学術講演会
    • Place of Presentation
      函館国際観光ホテル(函館市、北海道)
    • Year and Date
      2016-10-27
    • Related Report
      2016 Research-status Report
  • [Presentation] カンピロバクター食中毒の発生頻度は養鶏場ブロイラーの保菌率と相関する2016

    • Author(s)
      Sabike, I.I., 上村涼子, 桐野有美, 目堅博久, 関口 敏, Farid, A.S., 後藤義孝, 堀井洋一郎, 山崎 渉
    • Organizer
      第50回腸炎ビブリオシンポジウム
    • Place of Presentation
      大阪大学(吹田市、大阪)
    • Year and Date
      2016-10-20
    • Related Report
      2016 Research-status Report
  • [Presentation] Novel molecular diagnositic technique for rapid and sensitive detection of foot-and-mouth disease virus (FMDV)2016

    • Author(s)
      Yamazaki, W.
    • Organizer
      3rd International conference on biotechnology application in agriculture
    • Place of Presentation
      Sharm El-Sheikh, Egypt
    • Year and Date
      2016-04-05
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Analysis of chicken cecal contents by pyrosequencing to search probiotics for Campylobacter2016

    • Author(s)
      Yamazaki, W., Uemura,R., ekiguchi, S., Taniguchi, T., Misawa, N.
    • Organizer
      50th Conference Cholera and other bacterial enteric infections US-Japan cooperative medical science program
    • Place of Presentation
      Washington D.C., USA
    • Year and Date
      2016-01-11
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] 口蹄疫・アフリカ豚コレラの発生状況と高感度な簡易診断技術開発のフィージビリティスタディ2016

    • Author(s)
      山崎 渉
    • Organizer
      宮崎大学テニュアトラック推進機構主催セミナー
    • Place of Presentation
      宮日会館(宮崎市、宮崎県)
    • Related Report
      2016 Research-status Report
  • [Presentation] 鶏盲腸におけるカンピロバクターへのプロバイオティクス探索を目的としたパイロシークエンス解析2015

    • Author(s)
      山崎 渉 上村涼子 関口敏 谷口喬子 三澤尚明
    • Organizer
      第36回日本食品微生物学会総会
    • Place of Presentation
      川崎市教育文化会館(神奈川県川崎市)
    • Year and Date
      2015-11-12
    • Related Report
      2015 Research-status Report
  • [Presentation] 鶏盲腸におけるカンピロバクターへのプロバイオティクス探索を目的としたパイロシークエンス解析2015

    • Author(s)
      山崎 渉 上村涼子 関口敏 谷口喬子 三澤尚明
    • Organizer
      平成27年度日本獣医公衆衛生学会(九州地区)
    • Place of Presentation
      メルパルク熊本(熊本県熊本市)
    • Year and Date
      2015-10-16
    • Related Report
      2015 Research-status Report
  • [Presentation] 牛趾乳頭腫症病変部の治療前後における細菌叢の包括的比較解析2015

    • Author(s)
      山崎 渉 後藤恭宏 岡田啓司 谷口喬子 林 哲也 三澤尚明
    • Organizer
      第158回日本獣医学会総会
    • Place of Presentation
      北里大学(青森県十和田市)
    • Year and Date
      2015-09-07
    • Related Report
      2015 Research-status Report
  • [Presentation] Introduction of loop-mediated isothermal amplification (LAMP) assay for rapid and sensitive detection of microbial pathogens2015

    • Author(s)
      Yamazaki W
    • Organizer
      Molecular diagnosis in viral vesicular diseases
    • Place of Presentation
      Sao Paulo, Brazil
    • Year and Date
      2015-07-28
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] 牛趾乳頭腫症病変部の治療前後における細菌叢の比較解析.2014

    • Author(s)
      山崎 渉 谷口喬子 後藤恭宏 林 哲也 三澤尚明.
    • Organizer
      第 67 回日本細菌学会 九州支部総会.
    • Place of Presentation
      鹿児島市
    • Year and Date
      2014-09-05 – 2014-09-06
    • Related Report
      2014 Research-status Report
  • [Book] Ecology, virulence and detection of pathogenic and pandemic Vibrio parahaemolyticus2016

    • Author(s)
      Yamazaki, W. et.al.
    • Total Pages
      134
    • Publisher
      Frontiers Media
    • Related Report
      2016 Research-status Report
  • [Book] 大・中・小動物実験プロトコール2016

    • Author(s)
      山崎 渉他
    • Total Pages
      146
    • Publisher
      宮日文化情報センター
    • Related Report
      2016 Research-status Report
  • [Book] 大・中・小動物実験プロトコール2016

    • Author(s)
      宮崎大学動物実験プロトコール編集委員会 (山崎 渉他32名)
    • Total Pages
      146
    • Publisher
      宮日文化情報センター
    • Related Report
      2015 Research-status Report
  • [Remarks] 宮崎大学 農学部 獣医学科

    • URL

      http://www.agr.miyazaki-u.ac.jp/~vet/vet_HP1.htm

    • Related Report
      2017 Annual Research Report
  • [Remarks] 宮崎大学農学部獣医学科

    • URL

      http://www.agr.miyazaki-u.ac.jp/~vet/vet_HP1.htm

    • Related Report
      2014 Research-status Report
  • [Remarks] 宮崎大学産業動物防疫リサーチセンター

    • URL

      http://www.miyazaki-u.ac.jp/cadic/query/query_index.php

    • Related Report
      2014 Research-status Report
  • [Patent(Industrial Property Rights)] 複数のプライマーセットを組み合わせたLAMP法を用いたアフリカ豚コレラウイルスの迅速検出法2016

    • Inventor(s)
      山崎 渉
    • Industrial Property Rights Holder
      国立大学法人宮崎大学
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2016-112827
    • Filing Date
      2016-06-06
    • Related Report
      2016 Research-status Report

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Published: 2014-04-04   Modified: 2022-02-16  

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