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Development of cheap and quick diagnostic methods using muzzle skin of rabid dogs

Research Project

Project/Area Number 26450410
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Veterinary medical science
Research InstitutionKitasato University

Principal Investigator

PARK ChunHo  北里大学, 獣医学部, 准教授 (50383550)

Co-Investigator(Renkei-kenkyūsha) Inoue Satoshi  国立感染症研究所, 獣医科学部, 室長 (90213157)
Yamada Kentaro  大分大学, 医学部, 准教授 (70458280)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywords狂犬病 / フィリピン / 犬 / 脳 / 洞毛(触毛) / 病理診断法 / メルケル細胞 / 人獣共通感染症 / 鼻口部洞毛(触毛) / 病理学 / 皮膚 / 唾液腺 / 中枢神経系
Outline of Final Research Achievements

The direct fluorescent antibody test on fresh brain tissues is the gold standard for rabies virus antigen detection in dogs. However, this method is laborious and holds a high risk of virus exposure for the experimenter. In mammals, the tactile hair is a specialized touch organ that is abundant in the muzzle skin. Each tactile hair is equipped with more than 2,000 sensory nerve endings. Therefore, this organ is expected to serve as an alternative postmortem diagnostic material. In the present study, muzzle skins were obtained from rabid dogs and the target cells and localization of rabies virus antigen was defined. Virus antigen was clearly detected in a part of the outer root sheath at the level of the ring sinus of the tactile hairs, and the majority of cells were Merkel cell. Our results suggest that Merkel cells in the tactile hairs of the muzzle skin are a target for virus replication and could serve as a useful specimen source for diagnosis of rabies.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (26 results)

All 2017 2016 2015 2014 Other

All Int'l Joint Research (4 results) Journal Article (8 results) (of which Int'l Joint Research: 6 results,  Open Access: 8 results,  Acknowledgement Compliant: 3 results,  Peer Reviewed: 7 results) Presentation (14 results) (of which Int'l Joint Research: 5 results,  Invited: 1 results)

  • [Int'l Joint Research] 熱帯医学研究所 (RITM)(フィリピン)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] 台湾・家畜衛生試験所 (AHRI)(台湾)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] モンゴル・獣医学研究所(モンゴル)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] Veterinary Research Department/RITM/Department of Health(Philippines)

    • Related Report
      2015 Research-status Report
  • [Journal Article] Pathological lesions in the central nervous system and peripheral tissues of ddY mice with street rabies virus (1088 strain)2017

    • Author(s)
      Kimitsuki K, Yamada K, Shiwa N, Inoue S, Nishizono A, Park CH.
    • Journal Title

      J. Vet. Med. Sci.

      Volume: 79(6)

    • NAID

      130005695725

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Localization of the rabies virus antigen in Merkel cells in the follicle-sinus complexes of muzzle skins of rabid dogs2016

    • Author(s)
      Shimatsu T, Shinozaki H, Kimitsuki K, Shiwa N, Manalo DL, Perez RC, Dilig JE, Yamada K, Boonsriroj H, Inoue S, Park CH.
    • Journal Title

      J. Virol. Methods

      Volume: 237(40) Pages: 40-46

    • Related Report
      2016 Annual Research Report
    • Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] A pathological study of the salivary glands of rabid dogs in the Philippines.2016

    • Author(s)
      Boonsriroj H, Manalo DL, Kimitsuki K, Sihmatsu T, Shiwa N, Shinozaki H, Takahashi Y, Tanaka N, Inoue S, Park CH.
    • Journal Title

      J. Vet. Med. Sci.

      Volume: 78 Pages: 35-42

    • NAID

      130005121596

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Survey of francisella tularensis among wild animals in the endemic areas in Japan2016

    • Author(s)
      Hotta A, Tanabayashi K, Fujita O, Shindo J, Park CH, Kudo N, Hatai H, Oyamada T, Yamamoto Y, Takano A, Kawabata H, Sharma N, Uda A, Yamada A, Morikawa S.
    • Journal Title

      Jpn. J. Infect. Dis.

      Volume: inpress

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] ザーネン種山羊の脳脊髄糸状虫症の1例2016

    • Author(s)
      中本悠香、志和 希、 島津太一、君付和範、 富岡美千子,、片山芳也、筏井宏実、朴 天鎬
    • Journal Title

      日獣会誌

      Volume: 69 Pages: 36-40

    • NAID

      130005126759

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] The co-existence of a myxoid liposarcoma and leiomyoma in the same ovarian mass of a dog2016

    • Author(s)
      Shiwa N, Boonsriroj H, Kimitsuki K, Shimatsu T, Park CH.
    • Journal Title

      J. Vet. Med. Sci.

      Volume: 78 Pages: 467-471

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Congenital lobar emphysema concurrent with pneumothorax and pneumomediastinum in a dog2016

    • Author(s)
      Yun S, Lee H, Lim J, Lee K, Jang K, Shiwa N, Boonsriroj H, Kimitsuki K, Park CH, Kwon Y.
    • Journal Title

      J. Vet. Med. Sci.

      Volume: in press

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Association between RABV G proteins transported from the perinuclear space to cell surface membrane and N-glycosylation of the sequon at Asn2042015

    • Author(s)
      Hamamoto N, Uda A, Tobiume M, Park CH, Noguchi A, Kaku Y, Okutani A, Morikawa S, Inoue S
    • Journal Title

      JJID

      Volume: in press

    • NAID

      130005098925

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] フィリピンの熱帯医学研究所で狂犬病陽性と診断された発症犬の神経病理学的研究2017

    • Author(s)
      志和希、川本尚未、君付和範、Daria Llenaresas Manalo、井上智、 朴天鎬
    • Organizer
      第4回日本獣医病理学専門家協会学術集会
    • Place of Presentation
      タワーホール船堀(東京江戸川区)
    • Year and Date
      2017-03-30
    • Related Report
      2016 Annual Research Report
  • [Presentation] 狂犬病発症犬の鼻口部洞毛組織の死後診断的有用性の再確認と洞毛組織における病理学的所見2016

    • Author(s)
      中島千景, Daria Llenaresas Manalo, 君付和範, 川本尚未, 須見康代, 山岸光葉, 篠崎春美, 島津太一, 志和 希, 山田健太郎, 井上智, 朴天鎬
    • Organizer
      第159回日本獣医学会学術集会
    • Place of Presentation
      日本大学(神奈川県藤沢市)
    • Year and Date
      2016-10-04
    • Related Report
      2016 Annual Research Report
  • [Presentation] Merkel cells in the follicle-sinus complexes of muzzle skins are useful for postmortem diagnosis of rabid dogs2016

    • Author(s)
      Park CH, Shimatsu T, Kimitsuki K, Shiwa N, Manalo DL, Inoue S
    • Organizer
      The Korean Society of Veterinary Science
    • Place of Presentation
      Korea, Seoul
    • Year and Date
      2016-04-29
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Pathological study on the taste buds and von Ebner’s glands of rabid dogs in the Philippines2015

    • Author(s)
      Shiwa N, Sumi Y, Shimatsu T, Kimitsuki K, Boonsriroj H, Manalo DL, Inoue S, Park CH.
    • Organizer
      7th Asian Society of Veterinary Pathology (ASVP)
    • Place of Presentation
      University of the Philippines(Philippines)
    • Year and Date
      2015-11-07
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] A pathological study of the salivary glands of rabid dogs in the Philippines2015

    • Author(s)
      Kimitsuki K, Boonsriroj H, Manalo DL, Shimatsu T, Shiwa N, Takahashi Y, Tanaka N, Inoue S, Park CH.
    • Organizer
      7th Asian Society of Veterinary Pathology (ASVP)
    • Place of Presentation
      University of the Philippines(Philippines)
    • Year and Date
      2015-11-07
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] The utility of muzzle skin of rabid dogs as antemortem and postmortem diagnosis2015

    • Author(s)
      Shimatsu T, Kawamoto N, Shiwa N, Kimitsuki K, Boonsriroj H, Manalo DL, Shinozaki H, Takahashi Y, Tanaka N, Inoue S, Park CH.
    • Organizer
      7th Asian Society of Veterinary Pathology (ASVP)
    • Place of Presentation
      University of the Philippines(Philippines)
    • Year and Date
      2015-11-07
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Congenita lobar emphysema concurrent with pneumothorax and pneumomediastinum in a dog2015

    • Author(s)
      Kim E, Yun SH, Lim J, Jang KH, Park CH, Kwon YS
    • Organizer
      7th Asian Society of Veterinary Pathology (ASVP)
    • Place of Presentation
      University of the Philippines(Philippines)
    • Year and Date
      2015-11-07
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] 直接迅速免疫組織化学(DRIT)法を利用したフィリピンにおける狂犬病検査法の検討2015

    • Author(s)
      Manalo L. Daria, Bazartseren Boldbaatar, 朴 天鎬, 井上 智
    • Organizer
      第158回日本獣医学会学術集会
    • Place of Presentation
      北里大学十和田キャンパス(青森県十和田市)
    • Year and Date
      2015-09-07
    • Related Report
      2015 Research-status Report
  • [Presentation] 動物由来感染症を想定した解剖施設紹介と狂犬病発症犬の病理組織学2015

    • Author(s)
      朴天鎬
    • Organizer
      平成27年度狂犬病予防業務地方ブロック技術研修会
    • Place of Presentation
      岩手県環境保健研究センター(岩手県盛岡市)
    • Year and Date
      2015-06-22
    • Related Report
      2015 Research-status Report
    • Invited
  • [Presentation] Pathological Study on the Central Nerve System and Peripheral Tissues of ddY Mice Intramuscularly Infected with Street Rabies Virus (1088 Strain)2015

    • Author(s)
      Park, CH, Yamada, K, Kimitsuki, K, Yanogawa, S, Hassadin, B, Inoue, S, Nishizono, A.
    • Organizer
      17th International Conference on Emerging Infectious Diseases (EID)
    • Place of Presentation
      台湾中央研究院ACADEMICA SINICA(台北市)
    • Year and Date
      2015-01-26 – 2015-01-29
    • Related Report
      2014 Research-status Report
  • [Presentation] Pathological Studies on the Salivary Glands of Rabid Dogs2015

    • Author(s)
      Hassadin, B, Takahashi, Y, Tanaka, N, Shinozaki, H, Kimitsuki, K, Daria, L, Manalo, Inoue, S, Park, CH.
    • Organizer
      7th International Conference on Emerging Infectious Diseases (EID)
    • Place of Presentation
      台湾中央研究院ACADEMICA SINICA(台北市)
    • Year and Date
      2015-01-26 – 2015-01-29
    • Related Report
      2014 Research-status Report
  • [Presentation] Different intracellular localization of G protein between Kyoto strain (street virus) and CVS-26 strain (fixed virus) was associated with N-glycosylation of G protein at the site of 2042015

    • Author(s)
      Hamamoto N, Uda A, Noguchi A, Okutani A, Kaku Y, Park CH, Morikawa S, Inoue S.
    • Organizer
      17th International Conference on Emerging Infectious Diseases (EID)
    • Place of Presentation
      台湾中央研究院ACADEMICA SINICA(台北市)
    • Year and Date
      2015-01-26 – 2015-01-29
    • Related Report
      2014 Research-status Report
  • [Presentation] 狂犬病発病犬の口唇皮膚におけるウイルス抗原の局在と診断的有用性2014

    • Author(s)
      篠崎春美、高橋友里花、矢野川祥子、君付和範、Hassadin Boonsriroj、Daria Llenaresas Manalo、井上智、朴 天鎬
    • Organizer
      第156回日本獣医学会学術集会
    • Place of Presentation
      北海道大学(札幌)
    • Year and Date
      2014-09-09 – 2014-09-12
    • Related Report
      2014 Research-status Report
  • [Presentation] 狂犬病発病犬の唾液腺に関する病理学的研究2014

    • Author(s)
      高橋友里花、篠崎春美、矢野川祥子、君付和範、Hassadin Boonsriroj、Daria Llenaresas Manalo、井上智、朴天鎬
    • Organizer
      第156回日本獣医学会学術集会
    • Place of Presentation
      北海道大学(札幌)
    • Year and Date
      2014-09-09 – 2014-09-12
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2022-02-16  

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