Prevalence and evaluation of the pathogenicity of Shiga toxin producing Escherichia coli derived from wild deer in Japan.
| Project/Area Number |
26450412
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Veterinary medical science
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| Research Institution | Nihon University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
丸山 総一 日本大学, 生物資源科学部, 教授 (30181829)
横山 栄二 千葉県衛生研究所, 細菌研究室, 室長 (40370895)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 志賀毒素産生大腸菌 / 鹿 / ジビエ / 食中毒 / 猪 / O157 / ゲノム解析 |
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| Outline of Final Research Achievements |
Shiga toxin producing Escherichia coli (STEC) and STEC O157 were isolated from 38 (11.8%) (49 strains) and 9 (2.8%)(9 strains) out of 323 deer in Japan. Some strains contained several pathogenicity-related genes, such as stx, eaeA and hlyA. Phylogenetic analysis of the STEC O157 strains demonstrated that one and eight strains were grouped in Clades 7 and 12, respectively. The clades 7 and 12 included some strains derived from human patients with hemorrhagic- and watery- diarrhea, respectively.
Thirty-one STEC strains derived from deer were used for the phylogenetic analysis based on the patterns of the presences of 24 pathogenicity-related genes and it was revealed that 2 strains of O5 STEC were significantly related to the strains derived from human patients, suggesting the possibility that the deer strains could show pathogenicity to humans.
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Report
(4 results)
Research Products
(6 results)
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[Journal Article] Characterization of Shiga toxin-producing <i>Escherichia coli</i> from feces of sika deer (<i>Cervus nippon</i>) in Japan using PCR binary typing analysis to evaluate their potential human pathogenicity2017
Author(s)
Kabeya H, Sato S, Oda S, Kawamura M, Nagasaka M, Kuranaga M, Yokoyama E, Hirai S, Iguchi A, Ishihara T, Kuroki T, Morita-Ishihara T, Iyoda S, Terajima J, Ohnishi M, Maruyama S.
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Journal Title
Journal of Veterinary Medical Science
Volume: 79
Issue: 5
Pages: 834-841
DOI
NAID
ISSN
0916-7250, 1347-7439
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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