Molecular biology of ferret HEV and development of animal model for hepatitis E
| Project/Area Number |
26450419
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Veterinary medical science
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| Research Institution | National Institute of Infectious Diseases |
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Principal Investigator |
Li Tiancheng 国立感染症研究所, ウイルス第二部, 主任研究官 (90370957)
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| Co-Investigator(Kenkyū-buntansha) |
網 康至 国立感染症研究所, 動物管理室, 主任研究官 (10202699)
石井 孝司 国立感染症研究所, ウイルス第二部, 室長 (40280763)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 動物モデル / 人獣共通感染症 / フェレットHEV / E型肝炎 / Ferret HEV / 肝炎 / 細胞培養 / 病原性 / フェレット / ferret HEV |
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| Outline of Final Research Achievements |
In this study we using ferret HEV-LPs as antigen to examine ferret HEV-specific IgG and IgM antibodies and found that the wide distribution of ferret HEV infection in laboratory and pet ferrets and its likely introduction into Japan through importation. The complete genome of ferret HEV from laboratory ferrets imported from the United States was identified. A cell culture system for ferret HEV was established by using PLC/PRF/5 cells, and this system may be useful for understanding the mechanisms of replication, infection, immunization and virion morphogenesis of ferret HEV.
We also confirmed that that monkeys and rats are not susceptible to ferret HEV Infection, suggesting that the ferret HEV is not transmissible to humans. In addition, we confirmed that the ferret HEV infection induced acute hepatitis and persistent infection in ferrets, suggesting that the ferrets are a candidate animal model for immunological as well as pathological studies of hepatitis E.
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Report
(4 results)
Research Products
(7 results)
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[Journal Article] Genetic and physicochemical analyses of a novel ferret hepatitis E virus, and clinical signs of infection after birth.2017
Author(s)
Li TC, Sayaka Yoshizaki, Michiyo Kataoka, Yasushi Ami, Yuriko Suzaki, Yen Hai Doan, Kei Haga, Koji Ishii, Naokazu Takeda, and Takaji Wakita.
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Journal Title
Infection, Genetic and revolution.
Volume: 51
Pages: 153-159
Related Report
Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Ferret Hepatitis E Virus Infection Induces Acute Hepatitis and Persistent Infection in Ferrets2016
Author(s)
Li Tiancheng, Yang T, Sayaka Yoshizaki, Kataoka M, Ami Y, Suzaki Y, Kishida N, Shirakura M, Imai M, Asanuma H, Takeda N, Wakita T
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Journal Title
Vet Microbiol
Volume: 183
Pages: 30-36
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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