Mechanistic study of the role of MUC1-like molecule in malignant proliferation of canine hemangiosarcomas

• Project/Area Number: 26450426
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Veterinary medical science
• Research Institution: Gifu University
• Principal Investigator: Sakai Hiroki 岐阜大学, 応用生物科学部, 准教授 (40283288)
• Co-Investigator(Kenkyū-buntansha): 村上 麻美 岐阜大学, 応用生物科学部, 助教 (30597125)
• Research Collaborator: Shehata Ibrahim Anwar
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 血管肉腫 / 犬 / Peroxiredoxin 6 / RNA干渉 / 培養細胞 / アポトーシス / Peroxireduxin 6 / peroxiredoxin 6

Outline of Final Research Achievements

Immunohistochemically, canine hemangiosarcomas were strongly positive using commercially provided anti human MUC1 antibody. In canine hemangiosarcoma cell lines, lesser molecular weight band (22kDa) was detected with Western blotting. Peptide sequencing after immunoprecipitation with the antibody revealed the antigen was canine peroxiredoxin 6(Prdx6). Apoptosis was induced in canine hemangiosarcoma cell lines by Prdx6 knockdown with small interfering RNA method. uPA/uPAR, which associating with Prdx6 in malignant proliferation and invasion, were strongly positive in canine hemangiosarcomas. In conclusion, Prdx6 and uPA/uPAR may associate with in malignant proliferation of canine hemangiosarcomas.

Research Products

• [Journal Article] Overexpression of Peroxiredoxin 6 Protects Neoplastic Cells against Apoptosis in Canine Haemangiosarcoma. (2016)
  • Author(s): Anwar, Sh., Yanai, T., Sakai, H.
  • Journal Title: Journal of Comparative Pathology Volume: 155 Issue: 1 Pages: 29-39
  • DOI: 10.1016/j.jcpa.2016.05.002
  • Peer Reviewed / Open Access
• [Journal Article] Immunohistochemical Detection of Urokinase Plasminogen Activator and Urokinase Plasminogen Activator Receptor in Canine Vascular Endothelial Tumours (2015)
  • Author(s): Anwar, S., Yanai, T., Sakai, H
  • Journal Title: Journal of Comparative Pathology Volume: 153 Issue: 4 Pages: 278-282
  • DOI: 10.1016/j.jcpa.2015.07.003
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Presentation] Immunohistochemical detection of urokinase plasminogen activator and its receptor in canine hemangiosarcoma and cutaneous hemangioma (2014)
  • Author(s): Shehata Anwar, 酒井洋樹，柳井徳磨
  • Organizer: 第157回日本獣医学会学術集会
  • Place of Presentation: 札幌
  • Year and Date: 2014-09-09