| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Outline of Final Research Achievements |
It was considered that one of the mechanisms underlying the hypersensitive induction of the acrosome reaction may be due to an increased content of cAMP [cAMP]i in boar spermatozoa collected during summer. Addition of cholesterol or removal of bicarbonate in the semen extender did not affect [cAMP]i of spermatozoa. Thus, a mechanism that cholesterol delays the induction of the acrosome reaction may be the effect on the generation of cAMP during the process of the acrosome reaction raher than during storage. Addition of magnesium lowered the occurrence of the acrosome reaction. The staining method of sperm acrosomes with FITC-PNA was modified. Preincubation of boar spermatozoa with adipose-derived stem cells (ADSC) increased the ability of in vitro fertilizing ability. Cholesterol, magnesium and ADSC are prospective in applying to a 'therapy of boar spermatozoa' suffering from summer infertility.
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