Etiology of boar spermatozoa affected with summer infertility in an attempt to develop a novel therapy

• Project/Area Number: 26450427
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Veterinary medical science
• Research Institution: Gifu University
• Principal Investigator: Murase Tetsuma 岐阜大学, 応用生物科学部, 教授 (30303514)
• Co-Investigator(Kenkyū-buntansha): 原山 洋 神戸大学, 農学研究科, 教授 (30281140)
• Research Collaborator: SHIMIZU Kenji
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: ブタ / 夏季不妊症 / 精子 / 人工授精 / 体外受精 / 先体反応 / コレステロール / 重炭酸 / cAMP / 再生医療 / 冷蔵保存 / Ca / カルシウム / チロシンリン酸化

Outline of Final Research Achievements

It was considered that one of the mechanisms underlying the hypersensitive induction of the acrosome reaction may be due to an increased content of cAMP [cAMP]i in boar spermatozoa collected during summer. Addition of cholesterol or removal of bicarbonate in the semen extender did not affect [cAMP]i of spermatozoa. Thus, a mechanism that cholesterol delays the induction of the acrosome reaction may be the effect on the generation of cAMP during the process of the acrosome reaction raher than during storage. Addition of magnesium lowered the occurrence of the acrosome reaction. The staining method of sperm acrosomes with FITC-PNA was modified. Preincubation of boar spermatozoa with adipose-derived stem cells (ADSC) increased the ability of in vitro fertilizing ability. Cholesterol, magnesium and ADSC are prospective in applying to a 'therapy of boar spermatozoa' suffering from summer infertility.

Research Products

• [Presentation] Modifications of methodology for the staining of frozen-thawed Japanese Black bull sperm acrosomes with fluorescein isothiocyanate peanut agglutinin (FITC-PNA) (2016)
  • Author(s): Reza Rajabi Toustani，Quzi Sharmin Akter，星野洋一郎，向島幸司，坂口慎一，村瀬哲磨
  • Organizer: 第 159 回日本獣医学会学術集会
  • Place of Presentation: 藤沢
  • Year and Date: 2016-09-05
• [Presentation] Summer infertility of boar spermatozoa and its therapy by adipose-derived stem cells and cholesterol (2016)
  • Author(s): Yamamoto, T., Murase, T., Shimizu, K., Koide, A., Kuwahara, Y., Suzuki, S., Matsukawa, Y., Funahashi, Y. and Gotoh, M.
  • Organizer: 18th International Congress on Animal Reproduction
  • Place of Presentation: Tours, France
  • Year and Date: 2016-06-27
  • Int'l Joint Research
• [Presentation] Summer Infertility of Boar Spermatozoa and Its Therapy by Adipose-Derived Stem Cells and Cholesterol (2016)
  • Author(s): T. Yamamoto, T. Murase, K. Shimizu, A. Koide, Y. Kuwahara, S. Suzuki, Y. Matsukawa, Y. Funahashi and M. Gotoh
  • Organizer: 18th International Congress on Animal Reproduction
  • Place of Presentation: Le Vinci in Tours, France
  • Year and Date: 2016-06-26
  • Int'l Joint Research