Epidemiological study of influenza virus and hepacivirus among dogs and cats in Japan.

• Project/Area Number: 26450430
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Veterinary medical science
• Research Institution: Kagoshima University
• Principal Investigator: Matsuu Aya 鹿児島大学, 農水産獣医学域獣医学系, 准教授 (40348595)
• Co-Investigator(Renkei-kenkyūsha): MOMOI Yasuyuki 鹿児島大学, 農水産獣医学域獣医学系, 教授 (40303515)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: ウイルス感染症 / 伴侶動物 / 非霊長類ヘパシウイルス / 犬インフルエンザウイルス / インフルエンザ / 犬インフルエンザ / ヘパシウイルス / GLIPS法

Outline of Final Research Achievements

In this study, to elucidate the frequency and distribution of non-primate hepacivirus among horses and dogs in Japan, serum samples from horses and dogs across Japan were screened for anti-nonstructural 3 protein antibodies using the Gaussia luciferase immunoprecipitation system assay. In the result, Japanese horses exhibit a high prevalence of NPHV, while no dogs showed seroprevalnece.
Serum samples from dogs and cats across Japan were also screened for antibodies against type A influenza viruses isolated from human, horse, and dogs, using hemagglutination inhibition assays and neutralization assays.In the result, 2.0% and 2.1% of the dogs and cats were positive for the subtype H1N1 human seasonal influenza virus, and 0.4 % of dogs were positive for the subtype H3N8 virus.

Research Products

• [Journal Article] Genetic and serological surveillance for non-primate hepacivirus in horses in Japan (2015)
  • Author(s): Aya Matsuu, Seiji Hobo, Kunihide Ando, Takashi Sanekata, Fumio Sato, Yoshiro Endo, Tomohiko Amaya, Tomohiro Osaki, Masayuki Horie, Tatsunori Masatani, Makoto Ozawa, Kyoko Tsukiyama-Kohara
  • Journal Title: Veterinary Microbiology Volume: 179 Issue: 3-4 Pages: 219-227
  • DOI: 10.1016/j.vetmic.2015.05.028
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Genetic diversity of highly pathogenic H5N8 avian influenza viruses at a single overwintering site of migratory birds in Japan, 2014/15 (2015)
  • Author(s): Makoto Ozawa, Aya Matsuu, Kaori Tokorozaki, Masayuki Horie, Tatsunori Masatani, Hiroko Nakagawa, Kosuke Okuya, Toshiko Kawabata, Shigehisa Toda
  • Journal Title: Euro Surveillance Volume: 20 Issue: 20 Pages: 15-27
  • DOI: 10.2807/1560-7917.es2015.20.20.21132
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] 古い感染症と新しい感染症：今何が流行しているのか (2017)
  • Author(s): 松鵜 彩
  • Organizer: 第13回日本獣医内科学アカデミー学術大会
  • Place of Presentation: パシフィコ横浜（神奈川県・横浜市）
  • Year and Date: 2017-02-16
  • Invited
• [Presentation] 犬および猫におけるA型インフルエンザウイルス感染状況についての血清学的調査 (2016)
  • Author(s): 松鵜 彩、遠藤泰之、桃井康行、安藤匡子、馬場 希、小林行治、井口愛子、日笠喜朗
  • Organizer: 平成28年度日本小動物獣医学会（九州地区大会）
  • Place of Presentation: 千草ホテル（福岡県・北九州市）
  • Year and Date: 2016-10-16
• [Presentation] 知っておきたいちょっと変わった感染症 犬インフルエンザ (2016)
  • Author(s): 松鵜 彩
  • Organizer: 日本獣医内科学アカデミー
  • Place of Presentation: パシフィコ横浜（神奈川県、横浜市）
  • Year and Date: 2016-02-19
  • Invited
• [Presentation] 全国の馬における非霊長類由来ヘパシウイルスの感染状況 (2014)
  • Author(s): 松鵜 彩、帆保誠二、小澤 真、堀江真行、正谷達謄、實方 剛、佐藤文夫、遠藤祥郎、天谷友彦、小原恭子
  • Organizer: 第157回 日本獣医学会
  • Place of Presentation: 北海道大学（北海道札幌市）
  • Year and Date: 2014-09-09 – 2014-09-12