| Project/Area Number |
26450432
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Veterinary medical science
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| Research Institution | Rakuno Gakuen University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
樋口 豪紀 酪農学園大学, 獣医学群, 教授 (00305905)
今内 覚 北海道大学, 獣医学研究科, 准教授 (40396304)
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| Co-Investigator(Renkei-kenkyūsha) |
ENDOU DAIJI 酪農学園大学, 獣医学群, 教授 (40168828)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 乳房炎 / 有用微生物 / 乳腺感染制御 / 乳酸菌 / 乳体細胞 / Bifidobacterium breve / ウシ乳房炎 / 自然免疫 / 非薬物治療 / 乳房炎制御 / クリアランス / 乳牛 / Ｂｉｆｉｄｏｂａｃｔｅｒｉｕｍ ｂｒｅｖｅ / 牛乳房炎 / 白血球機能 |
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| Outline of Final Research Achievements |
The aim of this study was to evaluate the efficacy of lactic acid bacteria (LAB) by intramammary infusion for control of bovine mastitis. Methods: LAB, Bifidobacterium breve(3x10^9cells/ml) was infused quarters with mastitis of 48 lactating cows after milking once a day for 2 days. Pathogens, somatic cell counts (SCC), lactoferrin (LF) and IgG and A, chemiluminescence (CL), and mRNA expressions of Th1, 2- cytokines on milk cells were evaluated.
Results: SCC and CL responses were increased (P<0.05) in quarters by LAB infusion. LF and IgG,A were also induced in milk on day 3 to day 7. Cytokines IL-1β,TNF-α and IL-6 were elevated in milk by LAB infusion. I Environmental mastitis pathogens, coagulase negative staphylococci and streptococci, were eliminated 50-60% by LAB infusion. In mastitis quarters with no pathogens, SCC in 80% quarters returned to the recovered normal range on day 14 by LAB infusion. The use of LAB appears to be a possible approach for controlling bovine mastitis.
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