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Gliotransmitter release and its protective effects in spinal astrocytes

Research Project

Project/Area Number 26450440
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Integrative animal science
Research InstitutionHokkaido University

Principal Investigator

Otsuguro Ken-ichi  北海道大学, 獣医学研究科, 准教授 (40344494)

Co-Investigator(Renkei-kenkyūsha) YAMAGUCHI Soichiro  北海道大学, 大学院獣医学研究科, 助教 (50596864)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywordsアストロサイト / アデノシン / 硫化水素 / 脊髄 / グリア伝達物質 / 低酸素 / シスタチオニンβシンターゼ / シスタチオンβシンターゼ / 平衡ヌクレオシド輸送体 / 平衡ヌクレオチド輸送体 / ギャップジャンクションヘミチャネル / アデノシンキナーゼ
Outline of Final Research Achievements

Production and release mechanisms of adenosine and hydrogen sulfide (H2S), which are known to be neuroprotective agents, were examined in the cultured spinal astrocytes. Under the low Ca2+ conditions, ATP released by astrocytes was extracellularly degraded to adenosine, resulting in the increase in extracellular adenosine level. The astrocytic CBS (H2S synthase) expression was suggested to be controlled/maintained by the neurons. Adenosine and H2S affect cellular functions via the activation of receptors and ion channels. Under the ischemic conditions, it is suggested that the change in adenosine and H2S production and releases by the low Ca2+ and/or neuronal death is involved in disease onset and progression.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (31 results)

All 2016 2015 2014 Other

All Journal Article (7 results) (of which Peer Reviewed: 7 results,  Acknowledgement Compliant: 6 results) Presentation (22 results) (of which Int'l Joint Research: 6 results) Remarks (2 results)

  • [Journal Article] Endogenous 5-HT outflow from chicken aorta by 5-HT uptake inhibitors and amphetamine derivatives2016

    • Author(s)
      Delgermurun D, Ito S, Ohta T, Yamaguchi S, Otsuguro K
    • Journal Title

      Journal of Veterinary Medical Science

      Volume: 78 Issue: 1 Pages: 71-76

    • DOI

      10.1292/jvms.15-0146

    • NAID

      130005121579

    • ISSN
      0916-7250, 1347-7439
    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Hydrogen sulfide activates TRPA1 and releases 5-HT from epithelioid cells of the chicken thoracic aorta.2016

    • Author(s)
      Delgermurun D, Yamaguchi S, Ichii O, Kon Y, Ito S, Otsuguro K
    • Journal Title

      Comp. Biochem. Physiol. C Toxicol. Pharmacol.

      Volume: 187 Pages: 43-49

    • NAID

      120006337745

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] An adenosine kinase inhibitor, ABT-702, inhibits spinal nociceptive transmission by adenosine release via equilibrative nucleoside transporters in rat2015

    • Author(s)
      Ken-ichi Otsuguro, Yuki Tomonari, Saori Otsuka, Soichiro Yamaguchi, Yasuhiro Kon, Shigeo Ito
    • Journal Title

      Neuropharmacology

      Volume: 97 Pages: 160-170

    • DOI

      10.1016/j.neuropharm.2015.05.035

    • NAID

      120005906926

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Contribution of α2A-adrenoceptor subtype to effect of dexmedetomidine and xylazine on spinal synaptic transmission of mice2015

    • Author(s)
      Takeshi Kobayashi, Ken-ichi Otsuguro, Soichiro Yamaguchi, Shigeo Ito
    • Journal Title

      European Journal of Pharmacology

      Volume: 761 Pages: 321-329

    • DOI

      10.1016/j.ejphar.2015.06.020

    • NAID

      120005825580

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Bidirectional effects of hydrogen sulfide via ATP-sensitive K+ channels and transient receptor potential A1 channels in RIN14B cells2015

    • Author(s)
      Ayako Ujike, Ken-ichi Otsuguro K, Ryo Miyamoto, Soichiro Yamaguchi, Shigeo Ito
    • Journal Title

      European Journal of Pharmacology

      Volume: 764 Pages: 463-470

    • DOI

      10.1016/j.ejphar.2015.07.029

    • NAID

      120005906928

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Neuronal regulation of hydrogen sulfide-producing enzyme cystathionine β-synthase expression in rat spinal cord astrocytes.2015

    • Author(s)
      Ryo Miyamoto, Ken-ichi Otsuguro, Soichiro Yamaguchi, Shigeo Ito
    • Journal Title

      Neuroscience Research

      Volume: 印刷中

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Different mechanisms of extracellular adenosine accumulation by reduction of the external Ca2+ concentration and inhibition of adenosine metabolism in spinal astrocytes.2015

    • Author(s)
      Ryota Eguchi, Sanae Akao, Ken-ichi Otsuguro, Soichiro Yamaguchi, Shigeo Ito
    • Journal Title

      Journal of Pharmacological Sciences

      Volume: 印刷中

    • NAID

      120005661436

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] H2S-induced Ca2+ signals are increased by IL-1β in RIN-14B cells2016

    • Author(s)
      Ayako Ujike, Soichiro Yamaguchi, Shigeo Ito, Ken-ichi Otsuguro
    • Organizer
      The 4th Sapporo Summer Seminar for One Health (SaSSOH)
    • Place of Presentation
      北海道大学(札幌市)
    • Year and Date
      2016-09-20
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] H2S releases 5-HT from chicken thoracic chemoreceptor cells via the activation of TRPA12016

    • Author(s)
      Dugar Delgermurun, Soichiro Yamaguchi, Osamu Ichii, Yasuhiro Kon, Shigeo Ito, Ken-ichi Otsuguro
    • Organizer
      The 4th Sapporo Summer Seminar for One Health (SaSSOH)
    • Place of Presentation
      北海道大学(札幌市)
    • Year and Date
      2016-09-20
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Multiple ischemic like stimuli increase extracellular adenosine in rat spinal astrocytes2016

    • Author(s)
      Ryota Eguchi, Soichiro Yamaguchi, Ken-ichi Otsuguro
    • Organizer
      The 4th Sapporo Summer Seminar for One Health (SaSSOH)
    • Place of Presentation
      北海道大学(札幌市)
    • Year and Date
      2016-09-20
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] IL-1β enhances H2S-induced Ca2+ responses and 5-HT release in RIN14B cells2016

    • Author(s)
      Ayako Ujike, Soichiro Yamaguchi, Shigeo Ito, Ken-ichi Otsuguro
    • Organizer
      第59回日本神経化学大会
    • Place of Presentation
      福岡国際会議場(福岡市)
    • Year and Date
      2016-09-08
    • Related Report
      2016 Annual Research Report
  • [Presentation] Ischemic stimuli induce extracellular accumulation of adenosine in rat spinal cord2016

    • Author(s)
      Ryota Eguchi, Soichiro Yamaguchi, Ken-ichi Otsuguro
    • Organizer
      第59回日本神経化学大会
    • Place of Presentation
      福岡国際会議場(福岡市)
    • Year and Date
      2016-09-08
    • Related Report
      2016 Annual Research Report
  • [Presentation] Effect of H2S on 5-HT release from glomus cells in the chicken thoracic aorta2016

    • Author(s)
      DELGERUMURUN Dugar, 山口聡一郎, 市居修、昆泰寛、伊藤茂男、乙黒兼一
    • Organizer
      第159回日本獣医学会学術集会
    • Place of Presentation
      日本大学(藤沢市)
    • Year and Date
      2016-09-06
    • Related Report
      2016 Annual Research Report
  • [Presentation] H2S releases 5-HT via activation of TRPA1 in chemoreceptor cells of chicken thoracic aorta2016

    • Author(s)
      Dugar DELGERMURUN, Soichiro YAMAGUCHI, Osamu ICHII, Yasuhiro KON, Shigeo ITO, Ken-ichi OTSUGURO
    • Organizer
      第30回北海道薬物作用談話会
    • Place of Presentation
      北海道大学(札幌市)
    • Year and Date
      2016-07-31
    • Related Report
      2016 Annual Research Report
  • [Presentation] Extracellular accumulation of adenosine by ischemic stimulations in rat spinal astrocytes2016

    • Author(s)
      江口遼太、山口聡一郎、乙黒兼一
    • Organizer
      第89回日本薬理学会年会
    • Place of Presentation
      パシフィコ横浜(横浜市)
    • Year and Date
      2016-03-09
    • Related Report
      2015 Research-status Report
  • [Presentation] Effect of pro-inflammatory cytokine on H2S-induced Ca2+ responses in RIN14B cells2016

    • Author(s)
      氏家絢子、山口聡一郎、伊藤茂男、乙黒兼一
    • Organizer
      第89回日本薬理学会年会
    • Place of Presentation
      パシフィコ横浜(横浜市)
    • Year and Date
      2016-03-09
    • Related Report
      2015 Research-status Report
  • [Presentation] ラット脊髄における硫化水素産生酵素CBSの発現はニューロンにより維持される2015

    • Author(s)
      宮本亮、乙黒兼一、山口聡一郎、伊藤茂男
    • Organizer
      第88回日本生化学会大会
    • Place of Presentation
      神戸ポートアイランド(神戸市)
    • Year and Date
      2015-12-01
    • Related Report
      2015 Research-status Report
  • [Presentation] Ca2+ reduction accumulates extracellular adenosine in spinal cord2015

    • Author(s)
      Ryota Eguchi, Sanae Masumoto, Soichiro Yamaguchi, Shigeo Ito, Ken-ichi Otsuguro
    • Organizer
      The 3rd Sapporo Summer Seminar for One Health (SaSSOH)
    • Place of Presentation
      北海道大学(札幌市)
    • Year and Date
      2015-09-15
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Isolation of 5-HT-containing cells using fluorescence activated cell sorter2015

    • Author(s)
      Ayako Ujike, Soichiro Yamaguchi, Shigeo Ito, Ken-ichi Otsuguro
    • Organizer
      The 3rd Sapporo Summer Seminar for One Health (SaSSOH)
    • Place of Presentation
      北海道大学(札幌市)
    • Year and Date
      2015-09-15
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Estimate the cause of the species difference of the effectiveness of xylazine by docking simulation2015

    • Author(s)
      Takeshi Kobayashi, Soichiro Yamaguchi, Yoshihiro Ikenaka, Shigeo Ito, Ken-ichi Otsuguro
    • Organizer
      The 3rd Sapporo Summer Seminar for One Health (SaSSOH)
    • Place of Presentation
      北海道大学(札幌市)
    • Year and Date
      2015-09-15
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] ニューロンによるラット脊髄アストロサイトの硫化水素産生酵素の発現調節2015

    • Author(s)
      宮本亮、乙黒兼一、山口聡一郎、伊藤茂男
    • Organizer
      第158回日本獣医学会学術集会
    • Place of Presentation
      北里大学(十和田市)
    • Year and Date
      2015-09-07
    • Related Report
      2015 Research-status Report
  • [Presentation] Gap junction hemichannels contribute to extracellular accumulation of adenosine by external Ca2+ reduction in rat spinal astrocytes2015

    • Author(s)
      江口 遼太
    • Organizer
      第88回日本薬理学会年会
    • Place of Presentation
      名古屋国際会議場(名古屋市)
    • Year and Date
      2015-03-18 – 2015-03-20
    • Related Report
      2014 Research-status Report
  • [Presentation] Hydrogen sulfide production is controlled by neuronal signals in rat spinal cord astrocytes2015

    • Author(s)
      宮本 亮
    • Organizer
      第88回日本薬理学会年会
    • Place of Presentation
      名古屋国際会議場(名古屋市)
    • Year and Date
      2015-03-18 – 2015-03-20
    • Related Report
      2014 Research-status Report
  • [Presentation] 脊髄アストロサイトのCa2+除去下で見られる細胞外アデノシン増加におけるギャップジャンクションヘミチャネルの関与2014

    • Author(s)
      江口 遼太
    • Organizer
      第157回日本獣医学会学術集会
    • Place of Presentation
      北海道大学(札幌市)
    • Year and Date
      2014-09-09 – 2014-09-12
    • Related Report
      2014 Research-status Report
  • [Presentation] 細胞外Ca2+除去下における脊髄アストロサイトでの細胞外アデノシン増加へのギャップジャンクションヘミチャネルの関与2014

    • Author(s)
      江口 遼太
    • Organizer
      第28回北海道薬物作用談話会
    • Place of Presentation
      北海道大学(札幌市)
    • Year and Date
      2014-07-19
    • Related Report
      2014 Research-status Report
  • [Presentation] Cysteine aminotransferase and mercaptopyruvate sulfurtransferase contribute to H2S production in the peripheral nervous system2014

    • Author(s)
      Ryo Miyamoto
    • Organizer
      17th World Congress of Basic & Clinical Pharmacology
    • Place of Presentation
      Cape Town International Convention Centre (South Africa)
    • Year and Date
      2014-07-13 – 2014-07-18
    • Related Report
      2014 Research-status Report
  • [Presentation] Hydrogen sulfide bidirectionally modulates Ca2+ signals in RIN14B cells2014

    • Author(s)
      Ayako Ujike
    • Organizer
      17th World Congress of Basic & Clinical Pharmacology
    • Place of Presentation
      Cape Town International Convention Centre (South Africa)
    • Year and Date
      2014-07-13 – 2014-07-18
    • Related Report
      2014 Research-status Report
  • [Presentation] H2S production in peripheral neurons is mediated by cysteine aminotransferase and mercaptaopyruvate sulfurtransferase2014

    • Author(s)
      Ryo Miyamoto
    • Organizer
      3rd International Conference on H2S Biology and Medicine
    • Place of Presentation
      京都大学(京都市)
    • Year and Date
      2014-06-04 – 2014-06-06
    • Related Report
      2014 Research-status Report
  • [Presentation] The bidirectional effect of hydrogen sulfide on Ca2+ signals via opening KATP and TRPA1 channels in RIN14B cells2014

    • Author(s)
      Ayako Ujike
    • Organizer
      3rd International Conference on H2S Biology and Medicine
    • Place of Presentation
      京都大学(京都市)
    • Year and Date
      2014-06-04 – 2014-06-06
    • Related Report
      2014 Research-status Report
  • [Remarks] 北海道大学 大学院獣医学研究院 薬理学教室ホームページ

    • URL

      https://www.vetmed.hokudai.ac.jp/organization/pharmacol/

    • Related Report
      2016 Annual Research Report
  • [Remarks] 北海道大学大学院獣医学研究科薬理学教室ホームページ

    • URL

      http://www.vetmed.hokudai.ac.jp/organization/pharmacol/index.html

    • Related Report
      2015 Research-status Report 2014 Research-status Report

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Published: 2014-04-04   Modified: 2018-03-22  

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