| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
Temperature-sensitive TRP channels are function as peripheral temperature sensors expressed in several cells. During lactation, mammary epithelial cells express TRPV4 and TRPM8, which are activated at warm and cool temperature, respectively. However, it remains unclear whether TRPV4 and TRPM8 are involved in milk secretion in mammary epithelial cells. In this study, we activated the TRPV4 and TRPM8 by using specific chemical agonists in the culture model of lactating mammary epithelial cells. The results showed that activation of TRPV4 decreased the cell number and inhibited milk production in the mammary epithelial cells TRPM8 activation also down-regulated the milk secretion ability of the mammary epithelial cells without the decrease in cell number. These results indicate that milk secretion of the mammary epithelial cells is regulated by surrounding temperature through activation of TRPV4 or TRPM8.
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