Identification and clarification of chondroitin sulfate subtype which controls skeletal muscle differentiation
| Project/Area Number |
26450444
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Integrative animal science
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| Research Institution | Tottori University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
田村 純一 鳥取大学, 地域学部, 教授 (30221401)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 糖鎖 / 筋分化 / コンドロイチン硫酸 / 筋芽細胞 / デコリン / 骨格筋分化 / C2C12 / 筋分化関連因子群 / 細胞周期 / 筋管の形成 / 筋芽細胞C2C12 / 筋管形成 / 筋管係数 |
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| Outline of Final Research Achievements |
In order to clarify the control ability of skeletal muscle differentiation of chondroitin sulfate (CS), five types of CS (CS-A, B, C, D and E) were added to myoblast C2C12 which can reproduce skeletal muscle differentiation process, and differentiation was carried out for a certain period of time. The value of myotube fusion index (FI) calculated from the number of nuclei in the myotubes was used as an index of muscle differentiation. As a result, all CS subtypes used in this reserch suppressed myotube formation, among which CS-E showed remarkable suppression. Moreover, myotube FI decreased concentration-dependent manner. Conversely, knocking down the decorin of CS proteoglycan of C2C12, the muscular differentiation promoted in reverse. From the above, it has clarified that CS have a strong potential to regulate the muscle differentiation.
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Report
(4 results)
Research Products
(11 results)
