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Identification and clarification of chondroitin sulfate subtype which controls skeletal muscle differentiation

Research Project

Project/Area Number 26450444
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Integrative animal science
Research InstitutionTottori University

Principal Investigator

HOSAKA Yoshinao  鳥取大学, 農学部, 教授 (00337023)

Co-Investigator(Kenkyū-buntansha) 田村 純一  鳥取大学, 地域学部, 教授 (30221401)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords糖鎖 / 筋分化 / コンドロイチン硫酸 / 筋芽細胞 / デコリン / 骨格筋分化 / C2C12 / 筋分化関連因子群 / 細胞周期 / 筋管の形成 / 筋芽細胞C2C12 / 筋管形成 / 筋管係数
Outline of Final Research Achievements

In order to clarify the control ability of skeletal muscle differentiation of chondroitin sulfate (CS), five types of CS (CS-A, B, C, D and E) were added to myoblast C2C12 which can reproduce skeletal muscle differentiation process, and differentiation was carried out for a certain period of time. The value of myotube fusion index (FI) calculated from the number of nuclei in the myotubes was used as an index of muscle differentiation. As a result, all CS subtypes used in this reserch suppressed myotube formation, among which CS-E showed remarkable suppression. Moreover, myotube FI decreased concentration-dependent manner. Conversely, knocking down the decorin of CS proteoglycan of C2C12, the muscular differentiation promoted in reverse. From the above, it has clarified that CS have a strong potential to regulate the muscle differentiation.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (11 results)

All 2016 2015 Other

All Journal Article (1 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 1 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (8 results) Remarks (2 results)

  • [Journal Article] Degree of suppression of mouse myoblast cell line C2C12 differentiation varies according to chondroitin sulfate subtype2016

    • Author(s)
      Warita K, Oshima N, Tamura J, Okuda-Takeda N, Hosaka YZ.
    • Journal Title

      Marine Drugs

      Volume: 14 (10) Issue: 10 Pages: 193-193

    • DOI

      10.3390/md14100193

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Presentation] デコリン糖鎖はmyostatin産生を調節して筋分化を負に制御する、2016

    • Author(s)
      保坂善真
    • Organizer
      第12回北海道結合組織勉強会
    • Place of Presentation
      北海道江別市
    • Related Report
      2016 Annual Research Report
  • [Presentation] デコリン糖鎖であるコンドロイチン硫酸Bによる筋分化の制御2016

    • Author(s)
      保坂善真、三宅耶衣、割田克彦
    • Organizer
      第35回日本糖質学会年会
    • Place of Presentation
      高知県高知市
    • Related Report
      2016 Annual Research Report
  • [Presentation] 筋分化におけるコンドロイチン硫酸の役割と機能の解明2015

    • Author(s)
      大島奈々、割田克彦、田村純一、保坂善真
    • Organizer
      日本解剖学会第70回中国・四国支部学術集会
    • Place of Presentation
      松山市、愛媛大学
    • Year and Date
      2015-10-24
    • Related Report
      2015 Research-status Report
  • [Presentation] Comparative study of muscle regeneration in cardiotoxin and glycerol models2015

    • Author(s)
      Mohamed AA Mahdy、割田克彦、保坂善真
    • Organizer
      日本解剖学会第70回中国・四国支部学術集会
    • Place of Presentation
      松山市、愛媛大学
    • Year and Date
      2015-10-24
    • Related Report
      2015 Research-status Report
  • [Presentation] イカ由来コンドロイチン硫酸の骨形成能に関する研究2015

    • Author(s)
      保坂善真
    • Organizer
      資源の循環利用を考えるシンポジウム
    • Place of Presentation
      鳥取市、鳥取大学
    • Year and Date
      2015-10-16
    • Related Report
      2015 Research-status Report
  • [Presentation] 筋分化過程でのデコリンの機能と役割の解析2015

    • Author(s)
      三宅耶衣、割田克彦、西邑隆徳、保坂善真
    • Organizer
      第158回日本獣医学会学術集会
    • Place of Presentation
      十和田市、北里大学
    • Year and Date
      2015-09-07
    • Related Report
      2015 Research-status Report
  • [Presentation] Regulation of Myotubeformation by Decorin Sugar Chain2015

    • Author(s)
      MIyake Yae, Hosaka Yoshinao Z
    • Organizer
      The 5th Congress of Asian AVA
    • Place of Presentation
      Bali, Indonesia
    • Year and Date
      2015-02-11 – 2015-02-13
    • Related Report
      2014 Research-status Report
  • [Presentation] 筋分化におけるコンドロイチン硫酸の役割と機能の解明2015

    • Author(s)
      大島奈々
    • Organizer
      第337回 鳥取大学農学部獣医畜産集談会
    • Place of Presentation
      鳥取市 鳥取大学
    • Year and Date
      2015-01-30
    • Related Report
      2014 Research-status Report
  • [Remarks]

    • URL

      http://staff.muses.tottori-u.ac.jp/y-hosa/

    • Related Report
      2015 Research-status Report
  • [Remarks] 鳥取大学農学部ホームページ

    • URL

      http://muses.muses.tottori-u.ac.jp/staff/y-hosa.htm

    • Related Report
      2014 Research-status Report

URL: 

Published: 2014-04-04   Modified: 2018-03-22  

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