Elucidation of the structure and function of a novel chicken serum protein

• Project/Area Number: 26450448
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Integrative animal science
• Research Institution: Tokai University
• Principal Investigator: Matsushita Misao 東海大学, 工学部, 教授 (00165812)
• Co-Investigator(Kenkyū-buntansha): 浅沼 秀樹 国立感染症研究所, インフルエンザウイルス研究センター, 室長 (40333570)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: ニワトリ / 血清 / タンパク質 / スカベンジャー受容体システインリッチドメイン / cDNAクローニング / ニワトリ血清タンパク質 / アミノ酸配列 / 精製

Outline of Final Research Achievements

EW135 is a chicken egg white protein with 9 tandem repeats of group B scavenger receptor cysteine rich (SRCR) domain. We previously discovered a chicken serum protein named CS120 which reacts with anti-EW135 antibodies. The purpose of this project was to elucidate the structure and function of CS120. We developed a method for the purification of CS120 by using ion-exchange and affinity chromatographies. By cDNA cloning, the primary structure of CS120 has been determined. CS120 was found to consist of 8 tandem repeats of group B SRCR domain. CS120 bound to Staphylococcus aureus and protein A, while it did not show an anti-influenza virus activity.

Research Products

• [Journal Article] The complement system of elasmobranches revealed by liver transcriptome analysis of a hammerhead shark, Sphyrna zygaena (2016)
  • Author(s): M Goshima, R Sekiguchi, M Matsushita, M Nonaka
  • Journal Title: Developmental & Comparative Immunology Volume: 61 Pages: 13-24
  • DOI: 10.1016/j.dci.2016.03.009
  • Peer Reviewed
• [Journal Article] Dual modulating functions of thrombomodulin in the alternative complement pathway (2016)
  • Author(s): K Tateishi, M Imaoka, M Matsushita
  • Journal Title: BioScience Trends Volume: 10 Issue: 3 Pages: 231-234
  • DOI: 10.5582/bst.2016.01052
  • NAID: 130005167347
  • ISSN: 1881-7815, 1881-7823
  • Peer Reviewed / Open Access
• [Journal Article] Components of the lectin pathway of complement activation in paediatric patients of intensive care units (2016)
  • Author(s): AS Swierzko, A Szala-Pozdziej, DC Kilpatrick, M Sobocinski, K Chojnacka, A Sokolowska, M Michalski, K Mazerant, JC Jensenius, M Matsushita, WR Krajewski, J Szczapa, L Bak-Romaniszyn, K Zeman, M Cedzynski
  • Journal Title: Immunobiology Volume: 221 Issue: 5 Pages: 657-669
  • DOI: 10.1016/j.imbio.2016.01.003
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] CTRP6 is an endogenous complement regulator that can effectively treat induced arthritis. (2015)
  • Author(s): Murayama A.M, Kakuta S, Inoue A, Umeda N, Yonezawa T, Maruhashi T, Tateishi K, Ishigame H, Yabe R, Ikeda S, Seno A, Chi H-H, Hashiguchi Y, Kurata R, Tada T, Kubo S, Sato N, Liu Y, Hattori M, Saijo S, Matsushita M, Fujita T, Sumida T, Iwakura Y
  • Journal Title: Nature Communications Volume: 6 Issue: 1 Pages: 1-12
  • DOI: 10.1038/ncomms9483
  • NAID: 120006986869
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] No Strong Relationship Between Components of the Lectin Pathway of Complement and Susceptibility to Pulmonary Tuberculosis. (2015)
  • Author(s): Chalmers JD, Matsushita M, Kilpatrick DC, Hill AT
  • Journal Title: Inflammation Volume: 38 Issue: 4 Pages: 1731-1737
  • DOI: 10.1007/s10753-015-0150-0
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] New insights into the role of ficolins in the lectin pathway of innate immunity. (2015)
  • Author(s): Endo Y, Matsushita M, Fujita T
  • Journal Title: International Review of Cell and Molecular Biology Volume: 316 Pages: 49-110
  • DOI: 10.1016/bs.ircmb.2015.01.003
  • ISBN: 9780128022818
  • Peer Reviewed / Open Access
• [Journal Article] Serum ficolin-2 in hospitalised patients with community-acquired pneumonia. (2014)
  • Author(s): Chalmers JD, Fleming GB, Rutherford J, Matsushita M, Kilpatrick DC, Hill AT
  • Journal Title: Inflammation Volume: 37 Issue: 5 Pages: 1635-1641
  • DOI: 10.1007/s10753-014-9891-4
  • Peer Reviewed
• [Presentation] Isolation and characterization of ferret microfibril-associated glycoprotein 4 (2016)
  • Author(s): K Tateishi, H Asanuma, M Matsushita
  • Organizer: 第45回日本免疫学会
  • Place of Presentation: 沖縄県宜野湾市
  • Year and Date: 2016-12-05
• [Presentation] Molecular and functional characterization of guinea pig mannose-binding lectin (2016)
  • Author(s): Y Baba, C Ono, R Sekiguchi, K Tateishi, Y Morita, M Nonaka, M Matsushita
  • Organizer: XXVIth International Complement Workshop
  • Place of Presentation: 金沢市
  • Year and Date: 2016-09-04
  • Int'l Joint Research
• [Presentation] EW135, a chicken egg white protein with group B scavenger cycteine-rich domains, binds to Staphylococcus aureus and Protein A (2015)
  • Author(s): Yoo W, Tateishi K, Matsushita M
  • Organizer: 5th European Veterinary Immunology Workshop
  • Place of Presentation: ウイーン（オーストリア）
  • Year and Date: 2015-09-02
  • Int'l Joint Research