| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
The mechanisms of DNA methylation including genomic imprinting have not been clarified. It remains unknown how and when DNA methylation is catalyzed at specific DNA sequences. DNA methyltransferases DNMT3A2 and its co-factor DNMT3L are essential for genomic imprinting during gametogenesis and co-expression of them is observed only in germ cells. In this study, to obtain better understand the imprinting machinery, transgenic mice in which DNMT3A2 and DNMT3L are consistently expressed were produced. DNA methylation analysis showed that abnormal DNA methylation occurred at least in five regions but not at imprinted regions. In conclusion, DNA methyltransferase accessible regions differ between germ cell and somatic cell linages and unmethylated alleles of imprinted regions are protected against de novo methylation activity during ontogeny.
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