Exploration of DNA methyltransferase accessible region towards elucidation of the mechanisms for genomic imprinting
Project/Area Number |
26450449
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Integrative animal science
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Research Institution | Tokyo University of Agriculture |
Principal Investigator |
Yayoi Obata 東京農業大学, 生命科学部, 教授 (70312907)
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Project Period (FY) |
2014-04-01 – 2018-03-31
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Project Status |
Completed (Fiscal Year 2017)
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Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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Keywords | DNAメチル化 / 胚発生 / ゲノムインプリンティング / DNAメチル基転移酵素 / インプリンティング / 卵子 / エピジェネティクス / ゲノミックインプリンティング / 発生 |
Outline of Final Research Achievements |
The mechanisms of DNA methylation including genomic imprinting have not been clarified. It remains unknown how and when DNA methylation is catalyzed at specific DNA sequences. DNA methyltransferases DNMT3A2 and its co-factor DNMT3L are essential for genomic imprinting during gametogenesis and co-expression of them is observed only in germ cells. In this study, to obtain better understand the imprinting machinery, transgenic mice in which DNMT3A2 and DNMT3L are consistently expressed were produced. DNA methylation analysis showed that abnormal DNA methylation occurred at least in five regions but not at imprinted regions. In conclusion, DNA methyltransferase accessible regions differ between germ cell and somatic cell linages and unmethylated alleles of imprinted regions are protected against de novo methylation activity during ontogeny.
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Report
(5 results)
Research Products
(34 results)
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[Journal Article] Reconstitution in vitro of the entire cycle of the mouse female germ line.2016
Author(s)
Hikabe O, Hamazaki N, Nagamatsu G, Obata Y, Hirao Y, Hamada N, Shimamoto S, Imamura T, Nakashima K, Saitou M, Hayashi K
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Journal Title
Nature
Volume: 539
Issue: 7628
Pages: 299-303
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Presentation] Ectopic expression of DNMT3A2 and DNMT3L during embryogenesis leads to abnormal methylations at certain gene promoters but not at the imprinted loci2015
Author(s)
Sasaki K, Hara S, Yamakami R, Takeuchi S, Hasegawa S, Ogata M, Kono T, Obata Y.
Organizer
Gordon Research Conference 2015, Fertilization & Activation of Development
Place of Presentation
Holderness, NH, USA
Year and Date
2015-07-19
Related Report
Int'l Joint Research
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