| Project/Area Number |
26450451
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Integrative animal science
|
|---|
| Research Institution | Azabu University |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
宇佐見 誠 国立医薬品食品衛生研究所, 薬理部, 室長 (20223521)
田中 和明 麻布大学, 獣医学部, 准教授 (50345873)
森田 英利 岡山大学, 環境生命科学研究科, 教授 (70257294)
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
SUZUKI Takehito 麻布大学, 獣医学部, 准教授 (90532052)
|
|---|
| Project Period (FY) |
2014-04-01 – 2017-03-31
|
| Project Status |
Completed (Fiscal Year 2016)
|
| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
|
|---|
| Keywords | 一酸化窒素(NO) / S-ニトロシル化タンパク質 / アポトーシス / 子宮 / ラット / S-ニトロシル化 / プロテオミクス / スピントラップ・EPR解析 / S－ニトロシル化 |
|---|
| Outline of Final Research Achievements |
We examined the role of nitric oxide (NO) on apoptosis and S-nitrosylation that might affect apoptosis. To inhibit NO production, pregnant rats were administered an NO synthase inhibitor, L-NAME, apoptotic cells were observed in the endometrium of L-NAME-treated and -untreated rats. L-NAME-treatment significantly increased apoptosis and the expressions of cleaved-caspase-3 and X-linked inhibitor of apoptosis (XIAP), but significantly decreased thioredoxin (Trx) -1. Furthermore, L-NAME-treatment significantly induced S-nitrosylation (SNO) of cleaved caspase-3 and XIAP that were associated with a marked decline in Trx-1. These results suggest that apoptosis in the endometrium is positively and negatively regulated by NO through caspase and its inhibitor and S-nitrosylation of those factors during mid-gestation.
|
