| Project/Area Number |
26450456
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Integrative animal science
|
|---|
| Research Institution | Tottori University |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
山野 好章 鳥取大学, 農学部, 教授 (00182593)
竹内 崇 鳥取大学, 農学部, 教授 (20216849)
浅野 淳 鹿児島大学, 農水産獣医学域獣医学系, 教授 (90312404)
|
|---|
| Project Period (FY) |
2014-04-01 – 2017-03-31
|
| Project Status |
Completed (Fiscal Year 2016)
|
| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
|
|---|
| Keywords | ラクトフェリン / ラクトフェリシン / TLR / アポトーシス / 常在細菌 / 胚移植 / 胚着床 / 精子 / 男性不妊 / 生殖補助医療 / 生殖工学 / 新規機能性食品 |
|---|
| Outline of Final Research Achievements |
Experiments were designed to elucidate the mechanism of TLR mediated spermatogenesis by lactoferrin. We used mouse sperm as a material, co-cultured with bacterial cell component and lactoferrin, then examined cells induced to undergo apoptosis using immunohistological method, and analyzed the expression of genes related to apoptosis by biochemical method and examined. As a result, it was revealed that lactoferrin suppresses sperm apoptosis by bacteria and does not affect fertility and embryogenesis. From the above, it became clear that spermatogenesis and cell death caused by bacteria by lactoferrin lactoferricin were suppressed, and it was revealed that fertilization rate and implantation rate were improved.
|
