Acceleration an selection of somatic nuclear reprogramming based on nuclear remodelling

• Project/Area Number: 26450459
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Integrative animal science
• Research Institution: Kindai University
• Principal Investigator: TANI Tetsuya 近畿大学, 農学部, 講師 (70319763)
• Co-Investigator(Renkei-kenkyūsha): KASHIWAZAKI Naomi 麻布大学, 獣医学部, 教授 (90298232) ; ITO Junya 麻布大学, 獣医学部, 准教授 (30454143) ; FUKUDA Tomokazu 岩手大学, 理工学部, 教授 (40321640) ; FUKUHARA Takeshi 順天堂大学, 大学院医学研究科, 准教授 (20359673)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: リプログラミング / iPS細胞 / 核移植 / クローン / 初期化 / 体細胞核移植 / 卵母細胞

Outline of Final Research Achievements

Studies of nuclear reprogramming in somatic cell nuclear transfer (SCNT) and induced pluripotent stem cells (iPSCs) are good model for understanding for cell differentiation and dedifferentiation under epigenetic regulation. Efficiency of iPSCs reprogramming dramatically improved by fusion of specific amino acid transactivation domain and useful for vole and chickene. Reprogramming based on oocyte by SCNT does not required MAPK activity and improved clone embryo development by H3K9 specific demethylase Kdm4d overexpression in oocyte or somatic cell.

Research Products

• [Journal Article] Mitogen-Activated Protein Kinase Activity Is Not Essential for the First Step of Nuclear Reprogramming in Bovine Somatic Cell Nuclear Transfer. (2017)
  • Author(s): Tetsuya Tani, Yoko Kato
  • Journal Title: Cellular reprogramming Volume: 19(2) Issue: 2 Pages: 95-106
  • DOI: 10.1089/cell.2016.0044
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Chick derived induced pluripotent stem cells by the poly-cistronic transposon with enhanced transcriptional activity. (2017)
  • Author(s): Katayama M, Hirayama T, Tani T, Nishimori K, Onuma M, Fukuda T.
  • Journal Title: J Cell Physiol Volume: 印刷中 Issue: 2 Pages: 990-1004
  • DOI: 10.1002/jcp.25947
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Immortalized prairie vole-derived fibroblasts (VMF-K4DTs) can be transformed into pluripotent stem cells and provide a useful tool with which to determine optimal reprogramming conditions (2017)
  • Author(s): KATAYAMA Masafumi、HIRAYAMA Takashi、KIYONO Tohru、ONUMA Manabu、TANI Tetsuya、TAKEDA Satoru、NISHIMORI Katsuhiko、FUKUDA Tomokazu
  • Journal Title: Journal of Reproduction and Development Volume: 63 Issue: 3 Pages: 311-318
  • DOI: 10.1262/jrd.2016-164
  • NAID: 130007314611
  • ISSN: 0916-8818, 1348-4400
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Expression of Six Proteins Causes Reprogramming of Porcine Fibroblasts Into Induced Pluripotent Stem Cells with Both Active X Chromosomes (2016)
  • Author(s): Tomokazu Fukuda, Tetsuya Tani, Seiki Haraguchi, Keichiro Donai, Nobuyoshi Nakajima, Hirohide Uenishi, Takahiro Eitsuka, Makoto Miyagawa, Sanghoun Song, Mananu Onuma, Yumi Hoshino, Eimei Sato, and Arata Honda.
  • Journal Title: J. Cell. Biochem. Volume: 118 Issue: 3 Pages: 537-553
  • DOI: 10.1002/jcb.25727
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Developmental Competence of Vitrified-Warmed Bovine Oocytes at the Germinal-Vesicle Stage is Improved by Cyclic Adenosine Monophosphate Modulators during In Vitro Maturation (2015)
  • Author(s): Kenji Ezoe, Akiko Yabuuchi , Tetsuya Tani, Chiemi Mori, Tetsuya Miki, Yuko Takayama, Zeki Beyhan, Yoko Kato, Takashi Okuno, Tamotsu Kobayashi, Keiichi Kato
  • Journal Title: PLOS ONE Volume: 10（5） Issue: 5 Pages: e0126801-e0126801
  • DOI: 10.1371/journal.pone.0126801
  • Peer Reviewed / Open Access
• [Presentation] リプログラミング６因子の発現によるブタiPS細胞の作製と特徴 (2017)
  • Author(s): 福田智一, 谷哲弥, 原口清輝, 土内憲一郎, 中嶋信美, 上西博英, 永塚貴広, 宮川誠, 宋相憲, 大沼学, 星野由美, 佐藤英明, 本多新
  • Organizer: 第64回日本実験動物学会総会
  • Place of Presentation: ビッグパレットふくしま（福島県・郡山市）
  • Year and Date: 2017-05-25
• [Presentation] アメリカ平原ハタネズミ由来の人工多能性幹細胞の作成 (2016)
  • Author(s): 片山 雅史，平山 貴士，堀江 健吾，清野 透，土内 憲一郎，谷 哲弥，竹田 省，西森 克彦，福田 智一
  • Organizer: 第109回日本繁殖生物学会大会
  • Place of Presentation: 麻布大学（神奈川県・相模原市）
  • Year and Date: 2016-09-25
• [Presentation] Pre-fusion treatment with phytohaemaglutinin-P (PHA-P) increases relative expression of mitochondria related genes in somatic cell nuclear transfer embryos in pig (2016)
  • Author(s): Ndubuisi Samuel MACHEBE, Masaki HATA, Kohei ARIFUKU, Yurika NARUMIYA,Yuki ITANI, Kazuki OHATA, Tetsuya TANI and Yoko KATO
  • Organizer: The 17th Asian-Australasian Association of Animal Production Societies
  • Place of Presentation: ホテル日航福岡（福岡県・福岡市）
  • Year and Date: 2016-08-22
  • Int'l Joint Research
• [Presentation] Evaluation of the impact of pre-exposure to PHA on developmental efficiency of porcine PA and SCNT embryos (2016)
  • Author(s): Machebe Ndubuisi、Hata Masaki、Arifuku Kohei、Narumiya Yurika、Itani Yuki、Ohata Kazuki、Tani Tetsuya、Kato Yoko
  • Organizer: 日本畜産学会第121回大会
  • Place of Presentation: 日本獣医生命科学大学・東京都・武蔵野市
  • Year and Date: 2016-03-27
• [Presentation] Development of porcine cloned embryos produced using fibroblast and porcine induced pluripotent stem cells as nuclei donor cell types (2016)
  • Author(s): Ndubuisi Samuel MACHEBE , Masaki HATA , Kohei ARIFUKU , Yurika NARUMIYA, Yuki ITANI, Kazuki OHATA, Tomokazu FUKUDA, Tetsuya TANI and Yoko KATO
  • Organizer: International Symposium on the Future of Nuclear Transfer and Nuclear Reprogramming
  • Place of Presentation: 山梨大学・山梨県・甲府市
  • Year and Date: 2016-03-10
  • Int'l Joint Research
• [Presentation] 体外加齢ブタ未受精卵の生物学的特性と体外発生能の検討 (2014)
  • Author(s): 谷哲弥・加藤容子
  • Organizer: 第107回日本繁殖生物学会大会
  • Place of Presentation: 北海道帯広市
  • Year and Date: 2014-08-23