Molecular mechanisms of ABC transporter, Bcrp1, in the oxidative stress response in mouse embryonic stem cells

Research Project

Project/Area Number	26450460
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Integrative animal science
Research Institution	Kindai University
Principal Investigator	MITANI Tasuku 近畿大学, 先端技術総合研究所, 教授 (10322265)
Co-Investigator(Renkei-kenkyūsha)	HARATA Masahiko 東北大学, 大学院・農学研究科, 准教授 (70218642)
Project Period (FY)	2014-04-01 – 2017-03-31
Project Status	Completed (Fiscal Year 2016)
Budget Amount *help	¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000) Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000) Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000) Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords	ES細胞 / ABCトランスポーター / 酸化ストレス / Bcrp1 / 酸化ストレス応答
Outline of Final Research Achievements	ABC transporter, Bcrp1, defines the side population (SP) cell phenotype and seems presumptive functional regulator of stem cells. This study examined the function of Bcrp1 on the oxidative stress response of mouse ES cells. The results are summarized as follows: (1) transcriptional activator of Bcrp1 mRNA isoform specific to ES cells, (2) generation of SP cell subpopulation induced by Bcrp1 expression in ES cells, (3) induction of Bcrp1 expression and maintenance of pluripotent status by the oxidative stress response, and (4) visualization of the spatial arrangement of nuclear organization using 3D-FISH in early mouse embryos and ES cells.