Reduction of DNA fragmentation of freeze-dried boar sperm and elucidation of the repair mechanism for piglet production
| Project/Area Number |
26450462
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Integrative animal science
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| Research Institution | National Agriculture and Food Research Organization (2016)
National Institute of Agrobiological Sciences (2014-2015) |
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Principal Investigator |
KIKUCHI Kazuhiro 国立研究開発法人農業・食品産業技術総合研究機構, 生物機能利用研究部門 動物機能利用研究領域, 主席研究員 (20360456)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | ブタ / 精子 / 凍結乾燥 / DNA断片化 / 修復 |
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| Outline of Final Research Achievements |
Freeze-drying causes DNA fragmentation in boar spermatozoa. Reduction of the fragmentation can be possible by the treatment with trehalose, however, this reduction does not affect fertilization and embryonic development. When we checked the expression of 6 kinds of DNA repair genes after the injection into matured oocyte, no clear evidence was detected. However, the expression of some kinds of the genes were expressed as the maturational stages progressed. These results suggest that the expression of these repair genes during maturation may contribute for the DNA repair of the freeze-dried sperm after the injection to matured oocytes.
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Report
(4 results)
Research Products
(7 results)
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[Journal Article] Expression of DNA repair genes in porcine oocytes before and after fertilization by ICSI using freeze-dried sperm.2016
Author(s)
Men NT, Kikuchi K, Furusawa T, Dang-Nguyen TQ, Nakai M, Fukuda A, Noguchi J, Kaneko H, Viet Linh N, Xuan Nguyen B, Tajima A.
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Journal Title
Animal Scinence Jounral
Volume: 87
Issue: 11
Pages: 0-0
DOI
NAID
Related Report
Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
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[Presentation] ブタにおける配偶保存研究の取り組み2016
Author(s)
菊地和弘, Tamas Somfai, Nguyen Thi Men
Organizer
Cryopreservation Conference 2016
Place of Presentation
基礎生物学研究所(愛知県・岡崎市)
Year and Date
2016-11-10
Related Report
Invited
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