| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
Freeze-drying causes DNA fragmentation in boar spermatozoa. Reduction of the fragmentation can be possible by the treatment with trehalose, however, this reduction does not affect fertilization and embryonic development. When we checked the expression of 6 kinds of DNA repair genes after the injection into matured oocyte, no clear evidence was detected. However, the expression of some kinds of the genes were expressed as the maturational stages progressed. These results suggest that the expression of these repair genes during maturation may contribute for the DNA repair of the freeze-dried sperm after the injection to matured oocytes.
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