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Mechanisms of chemoreception and biosynthesis of bioactive substances in several fruit fly species

Research Project

Project/Area Number 26450466
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Insect science
Research InstitutionKyoto University

Principal Investigator

Ono Hajime  京都大学, 農学研究科, 助教 (70452282)

Co-Investigator(Renkei-kenkyūsha) MITSUNO Hidefumi  東京大学, 先端科学技術研究センター, 助教 (60511855)
Research Collaborator Hee Alvin K. W.  プトラ大学
Project Period (FY) 2014-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords果実害虫ミバエ類 / ミカンコミバエ / 誘引物質 / 植物揮発成分 / 嗅覚受容体 / 機能解析 / アフリカツメガエル卵母細胞 / 1-octen-3-ol / geranyl acetate / farnesene / ウリミバエ / ナスミバエ / 化学感覚受容体 / 味覚受容体
Outline of Final Research Achievements

The fruit fly species in the genus Bactrocera and Zeugodacus are highly destructive pests of various fruits. The reproductive and host-finding behaviors of this species are affected by several plant semiochemicals that are perceived through chemosensory receptors. However, the chemosensory mechanisms by which this perception occurs have not been fully elucidated. We identified the genes coding for chemosensory receptors of three fruit fly species, and succeeded to characterized olfactory receptors in the Oriental fruit fly Bactrocera dorsalis that respond to plant volatiles in a heterologous expression system comprising Xenopus oocytes. We further assessed the attraction of both female and male flies to four volatiles recognized as ligands for the two ORs, and found that when used in combination with visual cues, certain plant volatiles had a significant effect on the landing behavior of mated females.

Report

(5 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (22 results)

All 2018 2017 2016 2015 Other

All Int'l Joint Research (3 results) Journal Article (3 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 3 results,  Acknowledgement Compliant: 2 results) Presentation (13 results) (of which Int'l Joint Research: 4 results) Remarks (3 results)

  • [Int'l Joint Research] マレーシアプトラ大学(マレーシア)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] ボローニャ大学(イタリア)

    • Related Report
      2015 Research-status Report
  • [Int'l Joint Research] マギル大学(カナダ)

    • Related Report
      2015 Research-status Report
  • [Journal Article] Glue protein production can be triggered by steroid hormone signaling independent of the developmental program in Drosophila melanogaster2017

    • Author(s)
      Yuya Kaieda, Ryota Masuda, Ritsuo Nishida, MaryJane Shimell, Michael B.O’Connor, HajimeOno
    • Journal Title

      Developmental Biology

      Volume: 430 Issue: 1 Pages: 166-176

    • DOI

      10.1016/j.ydbio.2017.08.002

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Characterization of candidate intermediates in the Black Box of the ecdysone biosynthetic pathway in Drosophila melanogaster: Evaluation of molting activities on ecdysteroid-defective larvae2016

    • Author(s)
      Junki Saito, Ryota Kimura, Yuya Kaieda, Ritsuo Nishida, Hajime Ono
    • Journal Title

      Journal of Insect Physiology

      Volume: 93-94 Pages: 94-104

    • DOI

      10.1016/j.jinsphys.2016.09.012

    • NAID

      120006463758

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Drosophila 4EHP is essential for the larval-pupal transition and required in the prothoracic gland for ecdysone biosynthesis2016

    • Author(s)
      Luca Valzania, Hajime Ono, Marilena Ignesti, Valeria Cavaliere, Fabio Bernardi, Chiara Gamberi, Paul Lasko, Giuseppe Gargiulo
    • Journal Title

      Developmental Biology

      Volume: 410 Issue: 1 Pages: 14-23

    • DOI

      10.1016/j.ydbio.2015.12.021

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Presentation] ミカンバエの食害の有無によるウンシュウミカン成熟果の香気成分の比較2018

    • Author(s)
      菅野伸哉、小野肇
    • Organizer
      第62回日本応用動物昆虫学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] ミカンコミバエ嗅覚受容体の機能解析:寄主果実に含まれる揮発成分への応答2018

    • Author(s)
      宮崎仁美、大竹潤、光野秀文、尾崎克久、Alvin Kah-Wei Hee、神崎亮平、西田律夫、小野 肇
    • Organizer
      第62回日本応用動物昆虫学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Ecdysone 生合成における 3 位の酸 化還元の意義とCYP6T3 の機能との関係2017

    • Author(s)
      齊藤 惇基、小野 肇
    • Organizer
      第61回日本応用動物昆虫学会大会
    • Place of Presentation
      東京農工大学小金井キャンパス(東京都・小金井市)
    • Year and Date
      2017-03-28
    • Related Report
      2016 Research-status Report
  • [Presentation] ショウジョウバエにおける 糊タンパク質が発現する 時期と条件の解析2017

    • Author(s)
      小野 肇、海江田 祐也
    • Organizer
      第61回日本応用動物昆虫学会大会
    • Place of Presentation
      東京農工大学小金井キャンパス(東京都・小金井市)
    • Year and Date
      2017-03-28
    • Related Report
      2016 Research-status Report
  • [Presentation] Bactrocera 属ミバエの化学受容体の同定と発現組織の解析2017

    • Author(s)
      宮崎仁実、大竹潤、吉永直子、森直樹、西田律夫、小野肇
    • Organizer
      日本農芸化学会2017年度大会
    • Place of Presentation
      京都女子大学(京都府・京都市)
    • Year and Date
      2017-03-20
    • Related Report
      2016 Research-status Report
  • [Presentation] ウンシュウミカン幼果由来のテルペン類を利用したミカンバエの誘引法の開発2017

    • Author(s)
      大竹潤,東浦祥光,大野慧,吉永直子,森直樹,西田律夫,小野肇
    • Organizer
      日本農芸化学会2017年度大会
    • Place of Presentation
      京都女子大学(京都府・京都市)
    • Year and Date
      2017-03-20
    • Related Report
      2016 Research-status Report
  • [Presentation] ショウジョウバエにおけるエクダイソン生合成過程での3位の還元反応の意義2017

    • Author(s)
      齊藤惇基, 吉永直子, 森直樹, 小野肇
    • Organizer
      日本農芸化学会2017年度大会
    • Place of Presentation
      京都女子大学(京都府・京都市)
    • Year and Date
      2017-03-20
    • Related Report
      2016 Research-status Report
  • [Presentation] Expression of a glue protein, Salivary gland secretion 3, can be triggered by steroid hormone signaling independent of the developmental program in Drosophila melanogaster2017

    • Author(s)
      Yuya Kaieda, Ryota Masuda, Hajime Ono
    • Organizer
      4th Asia Pacific Drosophila Research Conference
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Expression of a glue protein, Salivary gland secretion 3, is triggered by steroid hormone independently from developmental timeline in Drosophila melanogaster2017

    • Author(s)
      Yuya Kaieda, Ryota Masuda, Ritsuo Nishida, MaryJane Shimell, Michael B. O’Connor, Hajime Ono
    • Organizer
      The 3rd International Insect Hormone Workshop 2017
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Functional characterization of olfactory receptors responding to plant volatiles in the Oriental fruit fly, Bactrocera dorsalis2017

    • Author(s)
      Hitomi Miyazaki, Jun Otake, Hidefumi Mitsuno, Katsuhisa Ozaki, Ryohei Kanzaki, Alvin Kah-Wei Hee, Ritsuo Nishida, Hajime Ono
    • Organizer
      Joint Meeting of the 33rd Annual Meeting of ISCE and the 9th meeting of APACE
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Bactrocera 属ミバエ類の誘引物質の開発と化学感覚受容体の探索2015

    • Author(s)
      小野肇
    • Organizer
      2015 年ケモビ研究会
    • Place of Presentation
      KKR 箱根宮の下(神奈川県足柄下郡)
    • Year and Date
      2015-11-14
    • Related Report
      2015 Research-status Report
  • [Presentation] Expression of a glue protein, Salivary gland secretion 3, is triggered by steroid hormone independently from developmental timeline in Drosophila melanogaster2015

    • Author(s)
      Yuya Kaieda, Ryota Masuda, Hajime Ono
    • Organizer
      The 2nd International Insect Hormone Workshop
    • Place of Presentation
      ハニア(ギリシャ共和国)
    • Year and Date
      2015-07-16
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Bactrocera 属ミバエ類の化学感覚受容体の探索2015

    • Author(s)
      宮崎 仁実・西田 律夫・小野 肇
    • Organizer
      第59回日本応用動物昆虫学会大会
    • Place of Presentation
      山形大学小白川キャンパス(山形市)
    • Year and Date
      2015-03-27 – 2015-03-28
    • Related Report
      2014 Research-status Report
  • [Remarks] 化学生態学

    • URL

      http://www.chemeco.kais.kyoto-u.ac.jp/

    • Related Report
      2017 Annual Research Report
  • [Remarks] 昆虫ホルモンの生合成と発育制御機構

    • URL

      http://www.chemeco.kais.kyoto-u.ac.jp/project_ecdysone.html

    • Related Report
      2016 Research-status Report
  • [Remarks] 京都大学・化学生態学研究室

    • URL

      http://www.chemeco.kais.kyoto-u.ac.jp/

    • Related Report
      2015 Research-status Report

URL: 

Published: 2014-04-04   Modified: 2022-02-16  

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