Study on the post-transcriptional regulation of mRNA by the insect virus proteins.

• Project/Area Number: 26450467
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Insect science
• Research Institution: Kyoto Institute of Technology
• Principal Investigator: Kotani Eiji 京都工芸繊維大学, 応用生物学系, 准教授 (10273541)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2014: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: カイコ / バキュロウイルス / タンパク質発現 / 遺伝子組換え / 核酸結合タンパク質 / 転写後調節 / 翻訳後調節 / イントロンレスmRNA / 病原体ー宿主間相互作用

Outline of Final Research Achievements

Bombyx mori nucleopolyhedrovirus nucleic acid-binding protein BRO family likely supports the regulation of mRNA. We have shown that BRO-B and BRO-E associate with cellular TIA-1 homolog (BmTRN-1), a translational regulator in silkworm cells during viral infection. Co-expression of BRO-B　and BRO-E synergistically led to a significant decrease in protein synthesis from a designed transcript carrying the 5’UTR of a cellular mRNA with no significant change of the transcript abundance. BRO family proteins were also shown to be involved in the regulation of protein synthesis by the study using the transgenic silkworms with middle silk glands expressing the BRO-A that influenced the sericin cocoon production of the silkworms. These results suggest that the BRO family could regulate protein synthesis in the silkworm individuals and the application to obtain the silkworms with a useful trait.

Research Products

• [Journal Article] Bioengineered silkworms with butterfly cytotoxin-modified silk glands produce sericin cocoons with a utility for a new biomaterial (2017)
  • Author(s): Otsuki, R., Mori, H., Kotani, E. et al.
  • Journal Title: Proceedings of the National Academy of Sciences of the United States of America Volume: １１４
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Bombyx mori nucleopolyhedrovirus nucleic acid binding proteins BRO-B and BRO-E associate with host T-cell intracellular antigen 1 homologue BmTRN-1 to influence protein synthesis during infection. (2015)
  • Author(s): Kotani, E., Muto, S., Ijiri, H., Mori, H.
  • Journal Title: Journal of General Virology Volume: 96 Issue: 7 Pages: 1947-1951
  • DOI: 10.1099/vir.0.000136
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Cell proliferation by silk gut incorporating FGF-2 protein microcrystals. (2015)
  • Author(s): Kotani, E., Yamamoto, N., Kobayashi, I., Uchino, K., Muto, S., Ijiri1, H., Shimabukuro, J., Tamura, T., Sezutsu, H., Mori, H.
  • Journal Title: Sci. Rep. Volume: 5 Issue: 1 Pages: 1-10
  • DOI: 10.1038/srep11051
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Bombyx mori nucleopolyhedrovirus nucleic acid-binding proteins BRO-B and BRO-E associate with host TIA-1 homolog BmTRN-1 to influence protein synthesis during infection (2015)
  • Author(s): Kotani, E., Muto, S., Ijiri, H., Mori, H
  • Journal Title: Journal of General Virology Volume: 96
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] モンシロチョウサイトトキシンの後部絹糸腺発現によるセリシン蚕の作出とセリシン繭の応用。 (2017)
  • Author(s): 小谷英治・大槻亮輔・瀬筒秀樹・高木圭子・森肇
  • Organizer: 平成29年度蚕糸昆虫機能利用学術講演会（日本蚕糸学会第87回大会）
  • Place of Presentation: つくば市
  • Year and Date: 2017-03-21
• [Presentation] 核多角体病ウイルスのBROタンパク質による宿主タンパク質発現制御 (2015)
  • Author(s): 小谷英治、武藤清佳、森 肇
  • Organizer: 日本蚕糸学会
  • Place of Presentation: 北海道札幌市
  • Year and Date: 2015-09-26
• [Patent(Industrial Property Rights)] ピエリシン１AのADPリボシル化ドメイン遺伝子及びセリシン繭 (2016)
  • Inventor(s): 小谷英治・大槻亮輔・森肇
  • Industrial Property Rights Holder: 国立大学法人京都工芸繊維大学
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2016-136614
  • Filing Date: 2016-07-11