Functional analysis of small GTP binding protein (Rab) in the insect brain

• Project/Area Number: 26450468
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Insect science
• Research Institution: Kobe University
• Principal Investigator: UNO TOMOHIDE 神戸大学, 農学研究科, 教授 (80240852)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: Bombyx mori / rab / insect / brain / histochemistry / bombyxin / Rab / 脳 / ボンビキシン / 昆虫 / 免疫組織化学 / 分泌 / 神経ペプチド / カイコ / Ｒａｂ / ホルモン / 組織染色 / 蚕 / 免疫組織染色

Outline of Final Research Achievements

Rab proteins are small monomeric GTPases/GTP- binding proteins and form the largest branch of Ras super-family. RabX4 and RabX6 are an insect-specific Rab proteins that have no close homolog in vertebrates. RabX4-like immunohistochemical reactivity (RabX4-ir) was restricted to neurons of the pars intercerebralis and dorsolateral protocerebrum in the brain. Corpus allatum is a neuronal organ to secrete neuropeptides synthesized in the brain into the hemolymph. In corpus allatum, RabX4-ir was co-localized with the Rab6-ir. RabX6-like immunohistochemical reactivity (RabX6-ir) was identified at the face of the testis, not in the spermatogonia, and was specifically detected at a pair of tritocerebral cells of the male brain. Furthermore, RNA interference of RabX6 was shown to decrease testicular growth. Double staining analysis of Rabs and insulin-like peptide, bombyxin indicate that 4 Rabs-ir are overlapped with bombyxin-ir in the brain and corpus allatumn of Bombyx mori.

Research Products

• [Journal Article] Localization and functional analysis of the insect-specific RabX4 in the brain of Bombyx mori (2017)
  • Author(s): Tomohide Uno, Masayuki Furutani, Katsuhiko Sakamoto, Yuichi Uno, Kengo Kanamaru, Akira Mizoguchi, Susumu Hiragaki, Makio Takeda
  • Journal Title: Archives of insect biochemistry and physiology Volume: 96 Issue: 1
  • DOI: 10.1002/arch.21404
  • Peer Reviewed
• [Journal Article] Functional characterization of CYP52G3 from Aspergillus oryzae and its application for bioconversion and synthesis of hydroxyl flavanone and steroids. (2016)
  • Author(s): T. Uno., T.Yanase.,H.Imaishi
  • Journal Title: Biotechnology and applied biochemistry Volume: 印刷中 Issue: 3 Pages: 385-391
  • DOI: 10.1002/bab.1496
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Rab proteins in the brain and corpus allatum of Bombyx mori (2016)
  • Author(s): Uno.T., Furutani,M.,Watanabe,C., Sakamoto.K., Uno,Y., Kanamaru,K.,
  • Journal Title: Histochemistry and Cell Biology Volume: 未定 Issue: 1 Pages: 59-69
  • DOI: 10.1007/s00418-016-1422-y
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Functional Characterization of CYP1A9 and CYP1C1 from Anguillus japonica (2015)
  • Author(s): Uno,T., Izumi,C., Takenaka,S., Yanase,T., Imaishi,H., Kanamaru,K., Yamagata,H., Kaminishi,Y., Itakura,T
  • Journal Title: Environmental Toxicology and Pharmacology Volume: 40 Issue: 2 Pages: 360-368
  • DOI: 10.1016/j.etap.2015.07.006
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Point mutation of Cytochrome P450 2A6 (a polymorphic variant CYP2A6.25) confers new substrate specificity towards flavonoids (2015)
  • Author(s): Uno,T., Ogura,C., Izumi,C., Nakamura,M., Yanase,T., Yamazaki,H., Ashida,H., Kanamaru,K., Yamagata,H., Imaishi H
  • Journal Title: Biopharmaceutics & Drug Disposition Volume: 36 Issue: 8 Pages: 552-563
  • DOI: 10.1002/bdd.1966
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] 昆虫の低分子量GTP結合蛋 白質Rabの機能解析 (2018)
  • Author(s): 宇野 知秀・尾崎屋 悠祐・金丸 研吾
  • Organizer: 日本応用動物学会
• [Presentation] カイコ脳及びアラタ体におけるRabとBombyxinに関する免疫組織化学的解析 (2018)
  • Author(s): 尾崎屋 悠祐・金丸 研吾 宇野知秀
  • Organizer: 平成30年度蚕糸・昆虫機能利用学術講演会
• [Presentation] 免疫組織染色法を用いたカイコ低分子量GTP結合蛋白質（Rab）の機能解析 (2016)
  • Author(s): 古谷 昌之
  • Organizer: 日本昆虫学第76回大会・第60回日本応用動物昆虫学会大会合同大会
  • Place of Presentation: 大阪府立大学(大阪府・堺市）
  • Year and Date: 2016-03-29
• [Presentation] 免疫組織化学的染色法を用いたカイコ脳における低分子量GTP結合蛋白質(Rab)の機能解析 (2016)
  • Author(s): 渡辺 千尋
  • Organizer: 平成２８年度蚕糸・昆虫機能利用学術講演会
  • Place of Presentation: 京都工芸繊維大学(京都府・京都市）
  • Year and Date: 2016-03-18
• [Presentation] 昆虫に特異的な低分子量GTP結合蛋白質（Ｒａｂ）の機能解析 (2016)
  • Author(s): 尾崎屋 悠祐
  • Organizer: 平成２８年度蚕糸・昆虫機能利用学術講演会
  • Place of Presentation: 京都工芸繊維大学(京都府・京都市）
  • Year and Date: 2016-03-18