| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
A female larva of honeybee develops into a queen or a worker depending on the diet provided during the larval period. To verify the possibility that sex determination gene is involved in this process of caste differentiation, gene expressions in the fat bodies of adult bees were analyzed. The results suggested that the expressions of two sex determination genes, transformer 2 and doublesex, differ between queen and worker bees. In addition, in order to investigate the epigenetic mechanism underling the task change of worker bees from nursing to foraging, I analyzed methylation profiles of DNA in the brains of worker bees and identified 439 genes of which methylation patterns change. I also attempted genome editing by CRISPR/Cas9 system and successfully knockout the gene encoding the major royal jelly protein 1.
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