| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
In this study, we generated transgenic plants that express a heterologous cellulase from filamentous fungi under the control of a drought-inducible promoter and signal peptides associated with extracellular trafficking. When the endo-glucanase (EG) coding sequence was connected to the promoter region of Wdhn13 gene and signal sequence of WLT10 from common wheat, high cellulase activities were detected in the transgenic Arabidopsis plants. The EG transgenic plants showed neither morphological abnormality nor sterility. These results indicate that expression of cell wall-degrading enzymes such as cellulase by the drought-inducible promoter is one of the ways to enhance the saccharification ability of cellulosic biomass without any alteration of plant growth towards efficient production of biofuels.
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