| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
Mammalian sperm possess a Golgi-derived exocytotic organelle, the acrosome, located on the apical region of head. We investigated genetically modified mice of acrosome protein ACRBP-W and its pre-mRNA alternative splicing variant ACRBP-V5. Consequently, the major function of ACRBP-W is to retain the inactive status of sperm serine-protease Acrosin until acrosomal exocytosis. ACRBP-V5 is involved in the formation and configuration of acrosomal granule during early spermiogenesis.Moreover, it was found that actin cytoskeleton of round spermatid was disrupted by the loss of ACRBP-V5. To observe in detail, we constructed transgenic mouse carrying the Lifeact probe which specifically recognized actin.
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