Dynamics of actin cytoskeleton during mammalian spermatogenesis
Project/Area Number |
26450499
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Applied molecular and cellular biology
|
Research Institution | University of Tsukuba |
Principal Investigator |
|
Project Period (FY) |
2014-04-01 – 2018-03-31
|
Project Status |
Completed (Fiscal Year 2017)
|
Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
Keywords | マウス / 精子形成 / 受精 / 選択的スプライシング / 先体形成 / 先体 / 細胞骨格 / アクロソーム / プロセシング |
Outline of Final Research Achievements |
Mammalian sperm possess a Golgi-derived exocytotic organelle, the acrosome, located on the apical region of head. We investigated genetically modified mice of acrosome protein ACRBP-W and its pre-mRNA alternative splicing variant ACRBP-V5. Consequently, the major function of ACRBP-W is to retain the inactive status of sperm serine-protease Acrosin until acrosomal exocytosis. ACRBP-V5 is involved in the formation and configuration of acrosomal granule during early spermiogenesis.Moreover, it was found that actin cytoskeleton of round spermatid was disrupted by the loss of ACRBP-V5. To observe in detail, we constructed transgenic mouse carrying the Lifeact probe which specifically recognized actin.
|
Report
(5 results)
Research Products
(16 results)
-
[Journal Article] Biogenesis of sperm acrosome is regulated by pre-mRNA alternative splicing of Acrbp in the mouse.2016
Author(s)
Kanemori Y, Koga Y, Sudo M, Kang W, Kashiwabara S, Ikawa M, Hasuwa H, Nagashima K, Ishikawa Y, Ogonuki N, Ogura A, Baba T.
-
Journal Title
Proc Natl Acad Sci U S A.
Volume: 113
Issue: 26
Pages: 3696-3705
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
-
[Journal Article] SCFFbl12 increases p21Waf1/Cip1 expression level through atypical ubiquitin chains synthesis2016
Author(s)
Tsuruta F, Takebe A, Haratake K, Kanemori Y, Kim J, Endo T, Kigoshi Y, Fukuda T, Miyahara M, Ebina M, Baba T, Chiba T
-
Journal Title
Mol. Cell Biol.
Volume: 36
Issue: 16
Pages: 2182-2194
DOI
NAID
Related Report
Peer Reviewed
-
-
-
-
-
[Presentation] Surfing and swimming of ejaculated sperm in the mouse oviduct2016
Author(s)
Ishikawa, Y., Usui, T., Yamashita, M., Kanemori, Y., Baba, T.
Organizer
SSR 2016 49th Annual Meeting
Place of Presentation
San Diego, California, U.S.A
Year and Date
2016-07-16
Related Report
Int'l Joint Research
-
[Presentation] Biogenesis of sperm acrosome is regulated by pre-mRNA alternative splicing of Acrbp in the mouse.2016
Author(s)
Nagashima, K., Kanemori, Y., Koga, Y., Sudo, M., Kang, W., Kashiwabara, S., Ikawa, M., Hasuwa, H., Ishikawa, Y., Ogonuki, N., Ogura, A., Baba, T.
Organizer
SSR 2016 49th Annual Meeting
Place of Presentation
San Diego, California, U.S.A
Year and Date
2016-07-16
Related Report
Int'l Joint Research
-
-
-
-
-
-
-
-