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In vivo analysis of longevity mechanism of transcription factor DAF-16/FOXO

Research Project

Project/Area Number 26450500
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Applied molecular and cellular biology
Research InstitutionUniversity of Tsukuba

Principal Investigator

DAITOKU Hiroaki  筑波大学, 生命領域学際研究センター, 講師 (30361314)

Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords転写因子 / 寿命 / 老化 / 線虫 / 転写 / FOXO / DAF-16
Outline of Final Research Achievements

Since transcription factor DAF-16 is required for lifespan extension in various long-lived mutants of the nematode C. elegans, it has been considered as a longevity gene. However, these results are based on genetic analysis and it is unclear whether transcriptional activity of DAF-16 is actually increased in long-lived mutants. Here we established novel in vivo luc system in C. elegans and demonstrated that there is a positive correlation between lifespan extension and DAF-16 activity.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (13 results)

All 2016 2015 2014

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Open Access: 1 results,  Acknowledgement Compliant: 2 results) Presentation (10 results)

  • [Journal Article] Non-transcriptional function of FOXO1/DAF-16 contributes to translesion DNA synthesis.2016

    • Author(s)
      Daitoku H., Kaneko Y., Yoshimochi K., Matsumoto K., Araoi S., Sakamaki J., Takahashi Y., and Fukamizu A.
    • Journal Title

      Mol. Cell. Biol.

      Volume: 36 Issue: 21 Pages: 2755-2766

    • DOI

      10.1128/mcb.00265-16

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] CTF18 interacts with replication protein A in response to replication stress2016

    • Author(s)
      Yuta Kaneko, Hiroaki Daitoku, Chihiro Komeno, Akiyoshi Fukamizu
    • Journal Title

      Molecular Medicine Reports

      Volume: 印刷中

    • NAID

      120007133106

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Detection of choline and phosphatidic acid (PA) catalyzed by Phospholipase D (PLD) using MALDI-QIT-TOF/MS with 9-aminoacridine matrix2014

    • Author(s)
      Park KE, Kim JD, Nagashima Y, Kako K, Daitoku H, Matsui M, Park GG, Fukamizu A
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: 78 Issue: 6 Pages: 981-988

    • DOI

      10.1080/09168451.2014.910102

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Presentation] 転写因子FOXO1/DAF-16の高グルコースに応答したアセチル化制御機構2016

    • Author(s)
      大徳浩照、小沼久里子、平田優介、坂井愛理、稲川貴之、深水昭吉
    • Organizer
      第89回日本生化学会大会
    • Place of Presentation
      東北大学(宮城県・仙台市)
    • Year and Date
      2016-09-25
    • Related Report
      2016 Annual Research Report
  • [Presentation] 線虫を用いた転写因子FOXO1の翻訳後修飾制御機構のin vivo解析2016

    • Author(s)
      大徳 浩照、小沼 久里子、平田 優介、新生 翔、深水 昭吉
    • Organizer
      Front Runner of Future Diabetes Research第5回研究発表会
    • Place of Presentation
      ホテル椿山荘東京(東京都・文京区)
    • Year and Date
      2016-07-23
    • Related Report
      2016 Annual Research Report
  • [Presentation] S期の紫外線DNA損傷応答におけるフォークヘッド型転写因子FOXO1/DAF-16の役割2016

    • Author(s)
      金子悠太、大徳浩照、深水昭吉
    • Organizer
      冬の若手ワークショップ
    • Place of Presentation
      山中湖 ホテル清渓(山梨県山中湖村)
    • Year and Date
      2016-02-05
    • Related Report
      2015 Research-status Report
  • [Presentation] ゲノム編集技術を用いた転写因子DAF-16の寿命制御機構の解明2016

    • Author(s)
      新生翔、大徳浩照、深水昭吉
    • Organizer
      冬の若手ワークショップ
    • Place of Presentation
      山中湖 ホテル清渓(山梨県山中湖村)
    • Year and Date
      2016-02-05
    • Related Report
      2015 Research-status Report
  • [Presentation] 長寿転写因子FOXO1/DAF-16のDNA損傷応答メカニズム2015

    • Author(s)
      大徳浩照、深水昭吉
    • Organizer
      BMB2015
    • Place of Presentation
      神戸ポートピアホテル(兵庫県神戸市)
    • Year and Date
      2015-12-04
    • Related Report
      2015 Research-status Report
  • [Presentation] 長寿転写因子FOXO1/DAF-16のグルコースに応答したアセチル化制御機構の解析2015

    • Author(s)
      小沼久里子、大徳浩照、深水昭吉
    • Organizer
      BMB2015
    • Place of Presentation
      神戸国際展示場(兵庫県神戸市)
    • Year and Date
      2015-12-04
    • Related Report
      2015 Research-status Report
  • [Presentation] 線虫におけるCRISPR/cas9ゲノム編集技術を用いた転写因子DAF-16の転写活性化能欠失変異体の樹立2015

    • Author(s)
      新生翔、大徳浩照、深水昭吉
    • Organizer
      BMB2015
    • Place of Presentation
      神戸国際展示場(兵庫県神戸市)
    • Year and Date
      2015-12-01
    • Related Report
      2015 Research-status Report
  • [Presentation] 長寿変異体における転写因子DAF-16の転写活性化能の評価2015

    • Author(s)
      新生 翔, 大徳 浩照, 金子 悠太, 廣田 恵子, 深水 昭吉
    • Organizer
      冬の若手ワークショップ 2015
    • Place of Presentation
      ホテル松本楼(群馬県渋川市伊香保町)
    • Year and Date
      2015-02-06
    • Related Report
      2014 Research-status Report
  • [Presentation] 長寿転写因子DAF-16のin vivoにおける制御機構の解明2014

    • Author(s)
      米野 千尋, 新生 翔, 大徳 浩照, 金子 悠太, 深水 昭吉
    • Organizer
      第87回 日本生化学会年会
    • Place of Presentation
      京都国際会館(京都市左京区)
    • Year and Date
      2014-10-17
    • Related Report
      2014 Research-status Report
  • [Presentation] 紫外線DNA損傷応答におけるフォークヘッド型転写因子FOXO1/DAF-16の役割2014

    • Author(s)
      金子 悠太, 大徳 浩照, 吉用 賢治, 松本 佳保里, 米野 千尋, 深水 昭吉
    • Organizer
      第87回 日本生化学会年会
    • Place of Presentation
      京都国際会館(京都市左京区)
    • Year and Date
      2014-10-17
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2018-03-22  

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