| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
In this research project, we revealed that potato tuber formation is regulated by a molecular mechanism similar to that of flowering regulation by florigen. In the regulation of photoperiodic flowering, FT, a florigen, induces flowering by making florigen activation complex (FAC) which is composed of FT, 14-3-3, and FD. StSP6A, a potato FT, has been shown to induce tuber formation (Navarro et al., 2011). To examine the molecular mechanism of tuber induction, we performed Y2H, BiFC, in vitro pull-down assay, and phenotypic analysis of transgenic potato plants. We revealed that StSP6A can make complex with potato 14-3-3 and StFDL1, a FD-like protein. Ectopic expression of StSP6A induced tuberization in a 14-3-3 dependent manner. Knock-down of StFDL1 significantly delayed tuberization. Considering them together, it was shown that StSP6A makes FAC-like complex termed Tuberigen Activation Complex (TAC) which is composed of StSP6A, 14-3-3, and StFDL1 to induce tuberization.
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