Analysis of retrograde signal via 12-oxo-phytodienoic acid
| Project/Area Number |
26450503
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied molecular and cellular biology
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| Research Institution | Tottori University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 12-オキソフィトジエン酸 / オキシリピン / レトログレードシグナル / レドックス / 熱ショック転写因子 |
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| Outline of Final Research Achievements |
To investigate the involvement of 12-oxo-phytodienoic acid (OPDA)in the regulation of Apx2 and HsfA2 expression under high light in Arabidopsis, I analyzed using fad3/fad7/fad8 mutants which can not synthesis OPDA. However, there was no significant difference in the transcript level of Apx2 and HsfA2 between the wild-type plants and fad/3fad7/fad8 mutants at 1 h after high light irradiation, suggesting that OPDA was not involved in these induction within 1 hour. RNA-seq analysis showed that the transcript levels of 239 genes were decreased (<2-fold) in the fad3/fad7/fad8 mutants compared with the wild-type plants. This finding suggests that the high light inducible expression of these genes was regulated by oxilipin containing OPDA.
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Report
(4 results)
Research Products
(8 results)
