| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
In order to investigate function of gamma-aminobutyric acid (GABA) shunt in response to abiotic stresses, we established various kinds of transgenic rice lines that overexpress a gene for GABA aminotransferase (GABA -T) or glyoxylate reductase. These transgenic rice plants were subjected to abiotic stresses such as low temperature, salt, drought and hydrogen peroxide (200 mM). Temperature or drought stresses resulted in increase in biomass from most of plants. After treatment of plants with oxidative or low temperature stresses, plants were planted in the soil to cultivate for one month. The transgenic plants overexpressing OsGABA-T with RecA-chloroplst transit signal could survive at the high frequency. Free amino acid analysis of this lines demonstrated the decrease in GABA shunt-related free amino acids such as Ala, Gly, Ser, Glu and GABA, expect for Gln.
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