Development of analytical methods for biological compounds suing pillar array columns

• Project/Area Number: 26460033
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Physical pharmacy
• Research Institution: The University of Tokyo
• Principal Investigator: Tsunoda Makoto 東京大学, 大学院薬学系研究科(薬学部), 講師 (10323453)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: クロマトグラフィー / アミノ酸 / マイクロ化学チップ / マイクロ化学分析システム / 生体分子 / カテコールアミン / 前処理 / 分析化学 / 蛍光

Outline of Final Research Achievements

Liquid chromatography is widely used for analysis of biological compounds. However, it is known that conventional chromatography has limitation of separation efficiency. Hence, I focused on pillar array column, which is theoretically proven to have higher separation ability. In this study, Low pressure-drop and low-dispersion turn was successfully developed.

Research Products

• [Journal Article] Liquid Chromatography Chip with Low-Dispersion and Low-Pressure-Drop Turn Structure Utilizing a Distribution-Controlled Pillar Array (2016)
  • Author(s): M. Isokawa, K. Takatsuki, Y.T. Song, K. Shih, K. Nakanishi, Z. Xie, D.H. Yoon, T. Sekiguchi, T. Funatsu, S. Shoji,
  • Journal Title: Analytical Chemistry Volume: 88 Issue: 12 Pages: 64856491-64856491
  • DOI: 10.1021/acs.analchem.6b01201
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Rapid quantitative method for the detection of phenylalanine and tyrosine in human plasma using pillar array columns and gradient elution (2016)
  • Author(s): Y. Song, K. Takatsuki, T. Sekiguchi, T. Funatsu, S. Shoji, M. Tsunoda
  • Journal Title: Amino Acids Volume: 48 Issue: 7 Pages: 1731-1735
  • DOI: 10.1007/s00726-016-2248-6
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Retention and Bandwidth Predictions by Fast Gradient Elution Chromatography Using a Pillar Array Column (2016)
  • Author(s): Y. Song, K. Takatsuki, T. Sekiguchi, T. Funatsu, S. Shoji, M. Tsunoda
  • Journal Title: CHROMATOGRAPHY Volume: 37 Issue: 3 Pages: 111-116
  • DOI: 10.15583/jpchrom.2016.008
  • NAID: 130005277408
  • ISSN: 1342-8284, 1348-3315
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Quantification of Biological Thiols in the Plasma of a Homocystinuria Model with Cystathionine β-Synthase Deficiency Utilizing Hydrophilic Interaction Liquid Chromatography and Fluorescence Detection (2016)
  • Author(s): Muneki Isokawa, Kentaro Kobayashi, Yurika Miyoshi, Masashi Mita, Takashi Funatsu, Kenji Hamase, and Makoto Tsunoda
  • Journal Title: CHROMATOGRAPHY Volume: 37 Issue: 3 Pages: 147-151
  • DOI: 10.15583/jpchrom.2016.015
  • NAID: 130005277429
  • ISSN: 1342-8284, 1348-3315
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Determination and characterization of total thiols in mouse serum samples using hydrophilic interaction liquid chromatography with fluorescence detection and mass spectrometry (2016)
  • Author(s): M. Isokawa, T. Shimosawa, T. Funatsu, M. Tsunoda
  • Journal Title: J. Chromatogr. B Volume: 1019 Pages: 59-65
  • DOI: 10.1016/j.jchromb.2015.11.038
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Evaluation of the Effects of Sample Solutions and Injector Wash Solutions on Separation Efficiency in Hydrophilic Interaction Liquid Chromatography (2015)
  • Author(s): T. Kanamori, T. Funatsu, M. Tsunoda
  • Journal Title: CHROMATOGRAPHY Volume: 36 Issue: 3 Pages: 123-126
  • DOI: 10.15583/jpchrom.2015.028
  • NAID: 130005118201
  • ISSN: 1342-8284, 1348-3315
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Development of analytical method for catechol compounds in mouse urine using hydrophilic interaction liquid chromatography with fluorescence detection. (2015)
  • Author(s): T. Kanamori, M. Isokawa, T. Funatsu, M. Tsunoda
  • Journal Title: J. Chromatogr. B Volume: 985 Pages: 142-148
  • DOI: 10.1016/j.jchromb.2015.01.038
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Efficient Separation and Sensitive Detection of Biothiols by Hydrophilic Interaction Liquid Chromatography with Fluorescence Detection after Derivatization with 4-Aminosulfonyl-7-fluoro-2,1,3-benzoxadiazole (2014)
  • Author(s): M. Isokawa, T. Funatsu, M. Tsunoda
  • Journal Title: CHROMATOGRAPHY Volume: 35 Issue: 3 Pages: 169-172
  • DOI: 10.15583/jpchrom.2014.018
  • NAID: 130004715564
  • ISSN: 1342-8284, 1348-3315
  • Peer Reviewed / Open Access
• [Journal Article] Evaluation of the effects of sample dilution and volume in hydrophilic interaction liquid chromatography. (2014)
  • Author(s): M. Isokawa, T. Funatsu, M. Tsunoda
  • Journal Title: Chromatographia Volume: 77 Issue: 21-22 Pages: 1553-1556
  • DOI: 10.1007/s10337-014-2748-z
  • Peer Reviewed
• [Presentation] アミド修飾ピラーアレイカラムを用いた生体チオールのオンチップ分離 (2016)
  • Author(s): 磯川宗生, 中西完貴, Yoon Dong Hyun, 関口哲志, 船津高志, 庄子習一, 角田 誠
  • Organizer: 第14回次世代を担う若手のためのフィジカル・ファーマフォーラム
  • Place of Presentation: ロッジ舞洲（大阪府大阪市）
• [Presentation] Development of pillar array columns with low dispersion and low pressure drop turns (2016)
  • Author(s): Makoto Tsunoda, Muneki Isokawa, Katsuya Takatsuki, Tetsushi Sekiguchi, Takashi Funatsu, Shuichi Shoji
  • Organizer: 42nd International Symposium on High Performance Liquid Phase Separations and Related Techniques
  • Place of Presentation: San Francisco, USA
  • Int'l Joint Research
• [Presentation] DEVELOPMENT OF PILLAR ARRAY COLUMNS WITH LOW-DISPERSION TURNS (2016)
  • Author(s): Makoto Tsunoda
  • Organizer: 16th Asia-Pacific International Symposium on Microscale Separations and Analysis
  • Place of Presentation: Johor Bahr, Malaysia
  • Int'l Joint Research / Invited
• [Presentation] オンチップ液体クロマトグラフィーの開発と生体試料への応用 (2015)
  • Author(s): 角田 誠
  • Organizer: 第26回クロマトグラフィー科学会議
  • Place of Presentation: 九州大学（福岡県）
  • Year and Date: 2015-11-12
  • Invited
• [Presentation] Analysis of biological compounds using a pillar array column (2015)
  • Author(s): Makoto Tsunoda
  • Organizer: 21st International Symposium on Separation Sciences
  • Place of Presentation: Ljubljana, Slovenia
  • Year and Date: 2015-07-02
  • Int'l Joint Research
• [Presentation] Quantitative determination of branched-chain amino acids in human plasma using pilar array columns (2015)
  • Author(s): Yanting Song, Katsuya Takatsuki, Muneki Isokawa, Tetsushi Sekiguchi, Jun Mizuno, Takashi Funatsu, Shuichi Shoji, Makoto Tsunoda
  • Organizer: 42nd International Symposium on High Performance Liquid Phase Separations and Related Techniques
  • Place of Presentation: Geneva, Switzerland
  • Year and Date: 2015-06-22
  • Int'l Joint Research
• [Presentation] 親水性相互作用液体クロマトグラフィーによるカテコールアミン類の分析法の開発 (2014)
  • Author(s): 金森貴宏、船津高志、角田誠
  • Organizer: 第25回クロマトグラフィー科学会議
  • Place of Presentation: 京都
  • Year and Date: 2014-12-11
• [Presentation] Quantitative determination of branched-chain amino acids in human plasma using pillar array columns (2014)
  • Author(s): Makoto Tsunoda, Yanting Song, Katsuya Takatsuki, Tetsushi Sekiguchi, Jun Mizuno, Takashi Funatsu, Shuichi Shoji
  • Organizer: 14th Asia-Pacific International Symposium on Microscale Separation and Analysis
  • Place of Presentation: 京都
  • Year and Date: 2014-12-07
• [Presentation] 微量生体分子の高性能分離・高感度分析法の開発とその応用 (2014)
  • Author(s): 角田 誠
  • Organizer: 第58回日本薬学会関東支部大会
  • Place of Presentation: 東京
  • Year and Date: 2014-10-04
  • Invited
• [Patent(Industrial Property Rights)] ピラーアレイミキサー及びこれを使用した試料処理装置 (2017)
  • Inventor(s): 角田 誠 他
  • Industrial Property Rights Holder: 角田 誠 他
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2017-018648
  • Filing Date: 2017-02-03