Analysis of phosphorylation signal using a Phos-tag quencher
| Project/Area Number |
26460036
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Physical pharmacy
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| Research Institution | Hiroshima University |
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Principal Investigator |
Koike Tohru 広島大学, 医歯薬保健学研究院(薬), 教授 (90186586)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | プロテオミクス / 蛍光分析 / キナーゼ / フォスファターゼ / Phos-tag / リン酸化分子 / リン酸化プロテオミクス / 消光性分子 / フォスタグ / リン酸化反応 / 亜鉛錯体 / リン酸化 / 亜鉛化合物 |
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| Outline of Final Research Achievements |
By utilizing an original analytical method using Phos-tag derivatives, we have developed convenient and reliable protocols for real-time analysis of phosphorylation status of kinase or phosphatase substrates. Since the analytical volume of μM-level sample is between μL and mL, the general fluorometric apparatus can be used in a standard chemical laboratory. We believe that our Phos-tag technology will result in great progress in phophophroteomics.
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Report
(4 results)
Research Products
(12 results)
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[Journal Article] Expression and phosphorylation state analysis of intracellular protein kinases using Multi- PK antibody and Phos- tag SDS- PAGE.2015
Author(s)
Sugiyama, Y., Katayama, S., Kameshita, I., Morisawa, K., Higuchi, T., Todaka, H., Eiji Kinoshita, Kinoshita- Kikuta, E., Koike, T., Taniguchi, T., Sakamoto, S.
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Journal Title
MethodsX
Volume: 2
Pages: 469-474
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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