Development of target-specific secretion system and its application to DDS
| Project/Area Number |
26460041
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Physical pharmacy
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| Research Institution | Nagoya City University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | DDS / 人工細胞 / リポソーム / エクソサイトーシス |
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| Outline of Final Research Achievements |
In this study, we tried to develop the exocytosis-like secretion system that secretes intra-vesicular content in response to the recognition of specific molecule. To construct such a system, we tried to introduce Ca channel Orai-1 into a cell-sized giant liposome.
Orai-1 was expressed in E.coli and we succeeded in isolation and purification of Orai-1. We tried various ways to reconstitute the channel into the liposome, such as changing phospholipids that form liposomes and changing the preparetion methods of giant liposmes. However, we could not reconstitute enough channels into liposomes.
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Report
(4 results)
Research Products
(12 results)
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[Journal Article] Visualization of glucagon secretion from pancreatic α cells by bioluminescence video microscopy: identification of secretion sites in the intercellular contact regions2017
Author(s)
Yokawa, S., Suzuki, T., Inouye, S., Inoh, Y., Suzuki, R., Kanamori, T., Furuno, T., Hirashima, N.
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Journal Title
Biochem. Biophys. Res. Commun.
Volume: 485
Issue: 4
Pages: 725-730
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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