siRNA delivery to tumor by sequential injection of anionic polymer and siRNA lipoplex
Project/Area Number |
26460046
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Physical pharmacy
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Research Institution | Hoshi University |
Principal Investigator |
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Project Period (FY) |
2014-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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Keywords | siRNA / リポソーム / 肝転移がん / コンドロイチン硫酸 / 負電荷ポリマー / protein kinase N3 / ヒト乳がん / がん治療 / リポプレックス / 正電荷リポプレックス / ポリグルタミン酸 / がん |
Outline of Final Research Achievements |
We developed novel siRNA transfer method to the liver by sequential intravenous injection of anionic polymer and cationic liposome/siRNA complex (siRNA lipoplex). When siRNA lipoplex was intravenously injected into mice bearing liver metastasis, the accumulation of siRNA was mainly observed in the lungs. In contrast, when siRNA lipoplex was intravenously injected into mice bearing liver metastasis immediately after intravenous injection of chondroitin sulfate (CS), siRNA was accumulated in tumor-metastasized liver. In terms of a gene silencing effect, sequential injections of CS plus siRNA lipoplex could reduce expression of target gene in liver metastasis. Moreover, sequential injections of CS plus siRNA lipoplex of protein kinase N3 (PKN3) siRNA could suppress tumor growth in the mice bearing liver metastasis. From these findings, sequential injection of CS and siRNA lipoplex might be a novel systemic method of delivering siRNA to liver metastasis.
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Report
(4 results)
Research Products
(30 results)
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[Journal Article] siRNA Delivery into Tumor Cells by Cationic Cholesterol Derivative-Based Nanoparticles and Liposomes2015
Author(s)
Y. Hattori, E. Hara, Y. Shingu, D. Minamiguchi, A. Nakamura, S. Arai, H. Ohno, K. Kawano, N. Fujii, E. Yonemochi
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Journal Title
Biological and Pharmaceutical Bulletin
Volume: 38
Issue: 1
Pages: 30-38
DOI
NAID
ISSN
0918-6158, 1347-5215
Related Report
Peer Reviewed / Acknowledgement Compliant
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