| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
|---|
| Outline of Final Research Achievements |
We developed novel siRNA transfer method to the liver by sequential intravenous injection of anionic polymer and cationic liposome/siRNA complex (siRNA lipoplex). When siRNA lipoplex was intravenously injected into mice bearing liver metastasis, the accumulation of siRNA was mainly observed in the lungs. In contrast, when siRNA lipoplex was intravenously injected into mice bearing liver metastasis immediately after intravenous injection of chondroitin sulfate (CS), siRNA was accumulated in tumor-metastasized liver. In terms of a gene silencing effect, sequential injections of CS plus siRNA lipoplex could reduce expression of target gene in liver metastasis. Moreover, sequential injections of CS plus siRNA lipoplex of protein kinase N3 (PKN3) siRNA could suppress tumor growth in the mice bearing liver metastasis. From these findings, sequential injection of CS and siRNA lipoplex might be a novel systemic method of delivering siRNA to liver metastasis.
|
