| Project/Area Number |
26460048
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Physical pharmacy
|
|---|
| Research Institution | Hoshi University |
|---|
Principal Investigator |
|
|---|
| Research Collaborator |
HATTORI Yoshiyuki 星薬科大学, 薬学部, 准教授 (90350222)
KAWANO Kumi 星薬科大学, 薬学部, 助教 (20366834)
|
|---|
| Project Period (FY) |
2014-04-01 – 2017-03-31
|
| Project Status |
Completed (Fiscal Year 2016)
|
| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
|
|---|
| Keywords | 脂質ナノ粒子複合体 / siRNA / 小角Ｘ線散乱 / 等温滴定型力ロリメトリー / 凍結乾燥 / 安定性 / 脂質ナノ粒子 / 小角X線散乱測定 / 等温滴定型カロリメトリー / リポプレックス / ナノ粒子 / 小角散乱 / ITC / MADG / コレステロール誘導体 / 正電荷リポプレックス / 正電荷ナノプレックス |
|---|
| Outline of Final Research Achievements |
The lipid nanoparticle complex containing siRNA induced a higher gene expression suppressing effect than the other preparations. Complex formation process was examined by synchrotron radiation X-ray scattering measurement and isothermal titration calorimetry (ITC). The composite particles formed single lamellae, multilamellae, and aggregates depending on the number of double bonds in the carbon chain, and membrane density was high in lipids with low molecular bulk. Furthermore, as the lipid composition containing double bonds increased, the membrane density decreased and it was related to the ease of structural transformation.
As a result of examining the stability for nanoparticles against lyophilization which is a means for solid preparation, it was found that aggregation of fine particles after re-hydration can be reduced by addition of saccharide and polyamino acids.
|
