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Development of novel depot-forming bioabsorbable recombinant elastomers for immunoresponse augmentation

Research Project

Project/Area Number 26460050
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Physical pharmacy
Research InstitutionSt. Marianna University School of Medicine

Principal Investigator

Asai Daisuke  聖マリアンナ医科大学, 医学部, 助教 (10423485)

Co-Investigator(Kenkyū-buntansha) 福田 靖  国立感染症研究所, 細菌第二部, 主任研究官 (80270651)
Co-Investigator(Renkei-kenkyūsha) KATAYAMA Yoshiki  九州大学, 工学部, 教授 (70284528)
Research Collaborator MOROKUMA Kazunori  
SHIBAYAMA Keigo  
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords薬物デポ / 生体材料 / DDS / 注射ゲル / 徐放 / エラスチン / ワクチン抗原 / ワクチン / アジュバント
Outline of Final Research Achievements

The frequency of administration of antigen must be done for enhanced immunoresponses to repeated exposures to the same antigens. In the context of vaccine compliance, versatile prolonged-release antigen delivery platforms with single injection are needed. We approached this issue by in situ formation of antigen-containing implants using recombinant protein polymer hydrogels, and demonstrate its utility as an injectable antigen depot capable of sustained release of tetanus toxoid. The novelty of this work stems from the genetically encoded design of these peptide polymers, which enables key molecular parameters that control the properties of the hydrogel to be precisely specified at the genetic level for antigens. Our hydrogel system may hold the promise of tailor-made long-lasting depot systems for a variety of antigens, aiming to design single-injection-type of vaccines.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (20 results)

All 2016 2015 2014 Other

All Journal Article (8 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 8 results,  Open Access: 5 results,  Acknowledgement Compliant: 6 results) Presentation (10 results) Remarks (2 results)

  • [Journal Article] Role of amino acid residues surrounding the phosphorylation site in peptide substrates of G protein-coupled receptor kinase 2 (GRK2)2016

    • Author(s)
      Asai, D., Murata, M., Toita, R., Kawano, T., Nakashima, H., and Kang, J.H.
    • Journal Title

      Amino Acids

      Volume: 48 Issue: 12 Pages: 2875-2880

    • DOI

      10.1007/s00726-016-2345-6

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Reversal of efflux of an anticancer drug in human drug-resistant breast cancer cells by inhibition of protein kinase Cα (PKCα) activity2016

    • Author(s)
      Kim, C.W., Asai, D., Kang, J.H., Kishimura, A., Mori, T., and Katayama, Y.
    • Journal Title

      Tumor Biol.

      Volume: 37 Issue: 2 Pages: 1901-1908

    • DOI

      10.1007/s13277-015-3963-4

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Spatiotemporally photoradiation-controlled intratumoral depot for combination of brachytherapy and photodynamic therapy for solid tumor2016

    • Author(s)
      Mukerji, R., Schaal, J., Li, X., Bhattacharyya, J., Asai, D., Zalutsky, M.R, Chilkoti, A., and Liu, W.
    • Journal Title

      Biomaterials

      Volume: 79 Pages: 79-87

    • DOI

      10.1016/j.biomaterials.2015.11.064

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Potential new chemotherapy strategy for human ovarian carcinoma with a novel KSP inhibitor2015

    • Author(s)
      Takenaga, M., Yamamoto, Y., Takeuchi T., Ohta, Y., Tokura, Y., Hamaguchi, A., Asai, D., Nakashima, H., Oishi, S., and Fujii, N.
    • Journal Title

      Biochem. Biophys. Res. Commun.

      Volume: 463 Issue: 3 Pages: 222-228

    • DOI

      10.1016/j.bbrc.2015.05.029

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Monitoring of phosphorylated peptides by radioactive assay and matrix-assisted laser desorption-ionization time-of-flight mass spectrometry2015

    • Author(s)
      Kang, J.H., Asai, D., Toita, R., Kawano, T., and Murata, M.
    • Journal Title

      Amino Acids

      Volume: 47 Issue: 11 Pages: 2377-2383

    • DOI

      10.1007/s00726-015-2025-y

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Antibody internalization into living cell via crosslinker-mediated endocytosis2015

    • Author(s)
      Funamoto D, Asai D, Sato K, Yamaguchi Y, Kim CW, Sato H, Nakhaei E, Matsumoto S, Yoshikawa T, Sasaki K, Yamamoto T, Kishimura A, Mori T, and Katayama Y
    • Journal Title

      Chem. Lett.

      Volume: 44 Issue: 4 Pages: 468-470

    • DOI

      10.1246/cl.141157

    • NAID

      130005062125

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Tandemly repeated peptide for cancer-specific gene carrier prepared by native chemical ligation2015

    • Author(s)
      Funamoto D, Asai D, Kim CW, Nakamura Y, Lee EK, Nobori T, Niidome T, Mori T, and Katayama Y
    • Journal Title

      Chem. Lett.

      Volume: 44 Issue: 4 Pages: 474-476

    • DOI

      10.1246/cl.141121

    • NAID

      130005062141

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Expression and characterization of myristoylatedpreS1-conjugated nanocages for targeted cell delivery2014

    • Author(s)
      Murata M, Piao JS, Narahara S, Kawano T, Hamano N, Kang JH, Asai D, Ugawa R, Hashizume M,
    • Journal Title

      Protein Expressionand Purification

      Volume: 110 Pages: 52-56

    • DOI

      10.1016/j.pep.2014.12.001

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 人工エラスチンポリペプチド:破傷風トキソイドデポ製剤への最適化2016

    • Author(s)
      浅井大輔、福田靖、諸熊一則、山口優子、Ashutosh Chilkoti、柴山恵吾、中島秀喜
    • Organizer
      第32回日本DDS学会学術集会
    • Place of Presentation
      グランシップ(静岡県静岡市)
    • Year and Date
      2016-06-30
    • Related Report
      2016 Annual Research Report
  • [Presentation] 新規合成KSP阻害剤のヒト卵巣がんに対する抗腫瘍効果2016

    • Author(s)
      武永美津子、竹内智起、山本有貴、都倉享恵、新美純、濱口明美、太田有紀、浅井大輔、大石真也、中島秀喜、藤井信孝
    • Organizer
      日本薬学会第136年会
    • Place of Presentation
      パシフィコ横浜(横浜市西区)
    • Year and Date
      2016-03-26
    • Related Report
      2015 Research-status Report
  • [Presentation] マクロファージを選択的に認識する機能性ナノ分子の創製2015

    • Author(s)
      姜貞勲、戸井田力、浅井大輔、村田正治
    • Organizer
      第88回日本生化学大会/第38回日本分子生物学会 合同大会(BMB2015)
    • Place of Presentation
      神戸ポートアイランド(神戸市中央区)
    • Year and Date
      2015-12-01
    • Related Report
      2015 Research-status Report
  • [Presentation] 破傷風トキソイドを用いた人工エラスチン蛋白のアジュバントとしての可能性2015

    • Author(s)
      福田靖、浅井大輔、山口優子、諸熊一則、Ashutosh Chilkoti、中島秀喜、柴山恵吾
    • Organizer
      第19回日本ワクチン学会学術集会
    • Place of Presentation
      名鉄犬山ホテル(愛知県犬山市)
    • Year and Date
      2015-11-14
    • Related Report
      2015 Research-status Report
  • [Presentation] インジェクタブル人工エラスチンゲルによる抗HIVペプチドのデポ製剤化2015

    • Author(s)
      浅井大輔、金本大成、寺久保繁美、Ashutosh Chilkoti、武永美津子、中島秀喜
    • Organizer
      第37回日本バイオマテリアル学会大会
    • Place of Presentation
      京都テルサ(京都市南区)
    • Year and Date
      2015-11-09
    • Related Report
      2015 Research-status Report
  • [Presentation] インジェクタブル人工エラスチンによる抗原デポ剤:破傷風トキソイドの徐放効果の解析2015

    • Author(s)
      浅井大輔、福田靖、諸熊一則、Ashutosh Chilkoti、柴山恵吾、中島秀喜
    • Organizer
      第31回日本DDS学会学術集会
    • Place of Presentation
      京王プラザホテル(東京都新宿区)
    • Year and Date
      2015-07-02
    • Related Report
      2015 Research-status Report
  • [Presentation] ケミカルバイオロジー分子ツールの開発:ROCK2およびGRK2選択的ペプチド性基質2014

    • Author(s)
      浅井大輔、戸井田力、土谷享、村田正治、片山佳樹、姜貞勲、中島秀喜
    • Organizer
      第87回日本生化学大会
    • Place of Presentation
      京都国際会館、京都市左京区
    • Year and Date
      2014-10-15 – 2014-10-18
    • Related Report
      2014 Research-status Report
  • [Presentation] Development of peptide-based compounds for cytotoxicity induction utilizing ubiquitous endogenous IgG2014

    • Author(s)
      Funamoto, D., Park, S., Mori, T., Asai, D., Kishimura, A., Sato, K., Yamaguchi, Y., and Katayama, Y
    • Organizer
      The IUMRS International Conference in Asia 2014
    • Place of Presentation
      Fukuoka University, Fukuoka
    • Year and Date
      2014-08-24 – 2014-08-30
    • Related Report
      2014 Research-status Report
  • [Presentation] 新規KSP阻害剤の抗腫瘍効果に関する研究(第2報)2014

    • Author(s)
      武永美津子、竹内智紀、都倉享恵、濱口明美、山本有貴、太田有紀、浅井大輔、中島秀喜、大石真也、藤井信孝
    • Organizer
      第30回日本DDS学会学術集会
    • Place of Presentation
      慶応大学薬学部、東京都港区
    • Year and Date
      2014-07-30 – 2014-07-31
    • Related Report
      2014 Research-status Report
  • [Presentation] 内在性のIgGにより細胞毒性を誘導できるペプチド性化合物の開発2014

    • Author(s)
      船本大起、パクサウン、森健、浅井大輔、岸村顕広、佐藤一紀、山口容子、片山佳樹
    • Organizer
      第63回高分子学会年次大会
    • Place of Presentation
      名古屋国際会議場、名古屋市熱田区
    • Year and Date
      2014-05-28 – 2014-05-30
    • Related Report
      2014 Research-status Report
  • [Remarks] 抗微生物薬の送達手法開発(聖マリアンナ医科大学 微生物学教室)

    • URL

      http://www.marianna-u.ac.jp/microbiology/office/003334.html

    • Related Report
      2016 Annual Research Report
  • [Remarks] 抗微生物薬の送達手法開発(聖マリアンナ医科大学微生物学教室)

    • URL

      http://www.marianna-u.ac.jp/microbiology/office/003334.html

    • Related Report
      2015 Research-status Report 2014 Research-status Report

URL: 

Published: 2014-04-04   Modified: 2018-03-22  

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